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Selective immuno-supported liquid membrane (ISLM) extraction, enrichment and analysis of 2,4,6-trichlorophenol

Tudorache, Madalina LU and Emnéus, Jenny LU (2005) In Journal of Membrane Science 256(1-2). p.143-149
Abstract
2,4,6-Trichlorophenol (2,4,6-TCP) was extracted from liquid samples (standards and spiked river water) using immuno-supported liquid membrane (ISLM) extraction followed by fluorescence flow immunoassay (FFIA) detection of the immuno-extracted analyte. ISLM-FFIA involves simultaneous on-line sample clean-up, enrichment and detection of analytes. The analyte is transported from a continuously flowing aqueous sample phase (donor) through a supported liquid membrane (SLM), containing an immobilised organic solvent, to a stagnant aqueous phase (acceptor), containing excess anti-analyte antibody, where it is trapped as analyte-anti body complex. The acceptor content is then dispensed and mixed on-line with a fluorescent analyte tracer... (More)
2,4,6-Trichlorophenol (2,4,6-TCP) was extracted from liquid samples (standards and spiked river water) using immuno-supported liquid membrane (ISLM) extraction followed by fluorescence flow immunoassay (FFIA) detection of the immuno-extracted analyte. ISLM-FFIA involves simultaneous on-line sample clean-up, enrichment and detection of analytes. The analyte is transported from a continuously flowing aqueous sample phase (donor) through a supported liquid membrane (SLM), containing an immobilised organic solvent, to a stagnant aqueous phase (acceptor), containing excess anti-analyte antibody, where it is trapped as analyte-anti body complex. The acceptor content is then dispensed and mixed on-line with a fluorescent analyte tracer (2,4,6-TCP/NH/(CH2)(3)/CO/EDF) and the resulting labelled fraction, separated from the mixture by restricted access column, is an indirect measure of the analyte amount extracted in the acceptor. The performance of ISLM-FFIA was compared with a similar FFIA (without the extraction step) with the following results: the ISLM-FFIA configuration leads to improved assay sensitivity (LOD 27.92 +/- 2.44 and 224 +/- 15.86 mu g L-1 for ISLM-FFIA and FFIA, respectively) and improved selectivity and recovery for 2,4,6-TCP in presence of other chlorophenols (e.g. 2,4,5-trichlorophenol and 4-chlorophenol) compared to FFIA alone. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Membrane Science
volume
256
issue
1-2
pages
143 - 149
publisher
Elsevier
external identifiers
  • wos:000229616800017
  • scopus:19544377702
ISSN
0376-7388
DOI
10.1016/j.memsci.2005.01.045
language
English
LU publication?
yes
id
06d0913c-4f36-44f4-b336-55d91d539e28 (old id 151034)
date added to LUP
2007-06-28 09:22:15
date last changed
2017-07-30 04:42:56
@article{06d0913c-4f36-44f4-b336-55d91d539e28,
  abstract     = {2,4,6-Trichlorophenol (2,4,6-TCP) was extracted from liquid samples (standards and spiked river water) using immuno-supported liquid membrane (ISLM) extraction followed by fluorescence flow immunoassay (FFIA) detection of the immuno-extracted analyte. ISLM-FFIA involves simultaneous on-line sample clean-up, enrichment and detection of analytes. The analyte is transported from a continuously flowing aqueous sample phase (donor) through a supported liquid membrane (SLM), containing an immobilised organic solvent, to a stagnant aqueous phase (acceptor), containing excess anti-analyte antibody, where it is trapped as analyte-anti body complex. The acceptor content is then dispensed and mixed on-line with a fluorescent analyte tracer (2,4,6-TCP/NH/(CH2)(3)/CO/EDF) and the resulting labelled fraction, separated from the mixture by restricted access column, is an indirect measure of the analyte amount extracted in the acceptor. The performance of ISLM-FFIA was compared with a similar FFIA (without the extraction step) with the following results: the ISLM-FFIA configuration leads to improved assay sensitivity (LOD 27.92 +/- 2.44 and 224 +/- 15.86 mu g L-1 for ISLM-FFIA and FFIA, respectively) and improved selectivity and recovery for 2,4,6-TCP in presence of other chlorophenols (e.g. 2,4,5-trichlorophenol and 4-chlorophenol) compared to FFIA alone. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Tudorache, Madalina and Emnéus, Jenny},
  issn         = {0376-7388},
  language     = {eng},
  number       = {1-2},
  pages        = {143--149},
  publisher    = {Elsevier},
  series       = {Journal of Membrane Science},
  title        = {Selective immuno-supported liquid membrane (ISLM) extraction, enrichment and analysis of 2,4,6-trichlorophenol},
  url          = {http://dx.doi.org/10.1016/j.memsci.2005.01.045},
  volume       = {256},
  year         = {2005},
}