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Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates

Steinhauer, Cornelia LU ; Ressine, Anton LU ; Marko-Varga, György LU ; Laurell, Thomas LU ; Borrebaeck, Carl LU and Wingren, Christer LU (2005) In Analytical Biochemistry 341(2). p.204-213
Abstract
Major efforts to develop antibody microarray technology to enable global proteome analysis to be performed in a facile manner are under way. In this process, the design and the properties of the substrate will play crucial roles. In the present study, we have developed novel, highly biocompatible solid supports for microarrays, using adsorbed recombinant human single-framework antibody fragments as probes. Several silicon-based supports, including planar silicon, micro- and macroporous silicon, and nitrocellulose-coated variants thereof, were designed and evaluated in a stepwise procedure. The surfaces were scored based on biocompatibility and probe binding capacity as judged by spot morphology, signal intensities, signal to noise ratios,... (More)
Major efforts to develop antibody microarray technology to enable global proteome analysis to be performed in a facile manner are under way. In this process, the design and the properties of the substrate will play crucial roles. In the present study, we have developed novel, highly biocompatible solid supports for microarrays, using adsorbed recombinant human single-framework antibody fragments as probes. Several silicon-based supports, including planar silicon, micro- and macroporous silicon, and nitrocellulose-coated variants thereof, were designed and evaluated in a stepwise procedure. The surfaces were scored based on biocompatibility and probe binding capacity as judged by spot morphology, signal intensities, signal to noise ratios, dynamic range, sensitivity, and reproducibility. A set of five commercially available substrates, selected to represent a set of supports providing different surface and coupling chemistries, was used as reference surfaces. The results showed that several well-performing silicon-based supports could be designed; in particular, a nitrocellulose-coated macroporous variant, MAP3-NC7, received the highest scores. In comparison, MAP3-NC7 displayed properties equal to or better than those of the reference substrates. Taken together, designed surfaces based on silicon can undoubtedly meet the requirements of the next generation of solid supports for antibody microarrays. (c) 2004 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Biochemistry
volume
341
issue
2
pages
204 - 213
publisher
Elsevier
external identifiers
  • wos:000229460800002
  • pmid:15907865
  • scopus:19444378409
ISSN
1096-0309
DOI
10.1016/j.ab.2004.10.036
language
English
LU publication?
yes
id
be6552c4-8068-496e-9005-3406ac041617 (old id 151043)
date added to LUP
2007-06-27 16:23:06
date last changed
2017-09-10 03:39:47
@article{be6552c4-8068-496e-9005-3406ac041617,
  abstract     = {Major efforts to develop antibody microarray technology to enable global proteome analysis to be performed in a facile manner are under way. In this process, the design and the properties of the substrate will play crucial roles. In the present study, we have developed novel, highly biocompatible solid supports for microarrays, using adsorbed recombinant human single-framework antibody fragments as probes. Several silicon-based supports, including planar silicon, micro- and macroporous silicon, and nitrocellulose-coated variants thereof, were designed and evaluated in a stepwise procedure. The surfaces were scored based on biocompatibility and probe binding capacity as judged by spot morphology, signal intensities, signal to noise ratios, dynamic range, sensitivity, and reproducibility. A set of five commercially available substrates, selected to represent a set of supports providing different surface and coupling chemistries, was used as reference surfaces. The results showed that several well-performing silicon-based supports could be designed; in particular, a nitrocellulose-coated macroporous variant, MAP3-NC7, received the highest scores. In comparison, MAP3-NC7 displayed properties equal to or better than those of the reference substrates. Taken together, designed surfaces based on silicon can undoubtedly meet the requirements of the next generation of solid supports for antibody microarrays. (c) 2004 Elsevier Inc. All rights reserved.},
  author       = {Steinhauer, Cornelia and Ressine, Anton and Marko-Varga, György and Laurell, Thomas and Borrebaeck, Carl and Wingren, Christer},
  issn         = {1096-0309},
  language     = {eng},
  number       = {2},
  pages        = {204--213},
  publisher    = {Elsevier},
  series       = {Analytical Biochemistry},
  title        = {Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates},
  url          = {http://dx.doi.org/10.1016/j.ab.2004.10.036},
  volume       = {341},
  year         = {2005},
}