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Alterations in Lung Mast Cell Populations in Patients with COPD.

Andersson, Cecilia K LU ; Mori, Michiko LU ; Bjermer, Leif LU ; Löfdahl, Claes-Göran LU and Erjefält, Jonas LU (2010) In American Journal of Respiratory and Critical Care Medicine 181(3). p.206-217
Abstract
RATIONALE: Mast cells have important roles in innate immunity and tissue remodeling but have remained poorly studied in inflammatory airway diseases like COPD. OBJECTIVES: To perform a detailed histological characterization of human lung mast cell popu-lations at different severities of COPD, comparing with smoking and never-smoking controls. METHODS: Mast cells were analyzed in lung tissues from patients with mild to very severe COPD, GOLD IâIV (n = 25, 10 of whom were treated with corticosteroids). Never-smokers and smokers served as controls. The density, morphology and molecular characteristics of mucosal and connective tissue mast cells (MCT and MCTC, respectively) were analyzed in several lung regions. MEASUREMENTS AND MAIN RESULTS:... (More)
RATIONALE: Mast cells have important roles in innate immunity and tissue remodeling but have remained poorly studied in inflammatory airway diseases like COPD. OBJECTIVES: To perform a detailed histological characterization of human lung mast cell popu-lations at different severities of COPD, comparing with smoking and never-smoking controls. METHODS: Mast cells were analyzed in lung tissues from patients with mild to very severe COPD, GOLD IâIV (n = 25, 10 of whom were treated with corticosteroids). Never-smokers and smokers served as controls. The density, morphology and molecular characteristics of mucosal and connective tissue mast cells (MCT and MCTC, respectively) were analyzed in several lung regions. MEASUREMENTS AND MAIN RESULTS: In all compartments of COPD lungs, especially at severe stages, the MCTC population increased in density while the MCT population decreased. The net result was a reduction in total mast cell density. This phenomenon was paralleled by in-creased numbers of luminal mast cells whereas the numbers of TUNEL(+) apoptotic mast cells remained unchanged. In COPD lungs, the MCT and MCTC populations showed alterations in morphology and expression of CD88 (C5a-R), TGF-beta, and renin. Statistically significant cor-relations were found between several COPD-related mast cell alterations and lung function parameters. CONCLUSIONS: As COPD progresses to its severe stages, the mast cell population in the lung undergoes changes in density, distribution, and molecular expression. In COPD lungs, these novel histopathological features were found to be correlated to lung function and they may thus have clinical consequences. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Respiratory and Critical Care Medicine
volume
181
issue
3
pages
206 - 217
publisher
Am Thoracic Soc
external identifiers
  • wos:000273956600004
  • pmid:19926870
  • scopus:76749169127
ISSN
1535-4970
DOI
10.1164/rccm.200906-0932OC
language
English
LU publication?
yes
id
a71d8466-ab47-4e35-920b-d11e787d2eeb (old id 1511733)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19926870?dopt=Abstract
date added to LUP
2009-12-03 15:48:25
date last changed
2018-05-29 11:50:27
@article{a71d8466-ab47-4e35-920b-d11e787d2eeb,
  abstract     = {RATIONALE: Mast cells have important roles in innate immunity and tissue remodeling but have remained poorly studied in inflammatory airway diseases like COPD. OBJECTIVES: To perform a detailed histological characterization of human lung mast cell popu-lations at different severities of COPD, comparing with smoking and never-smoking controls. METHODS: Mast cells were analyzed in lung tissues from patients with mild to very severe COPD, GOLD IâIV (n = 25, 10 of whom were treated with corticosteroids). Never-smokers and smokers served as controls. The density, morphology and molecular characteristics of mucosal and connective tissue mast cells (MCT and MCTC, respectively) were analyzed in several lung regions. MEASUREMENTS AND MAIN RESULTS: In all compartments of COPD lungs, especially at severe stages, the MCTC population increased in density while the MCT population decreased. The net result was a reduction in total mast cell density. This phenomenon was paralleled by in-creased numbers of luminal mast cells whereas the numbers of TUNEL(+) apoptotic mast cells remained unchanged. In COPD lungs, the MCT and MCTC populations showed alterations in morphology and expression of CD88 (C5a-R), TGF-beta, and renin. Statistically significant cor-relations were found between several COPD-related mast cell alterations and lung function parameters. CONCLUSIONS: As COPD progresses to its severe stages, the mast cell population in the lung undergoes changes in density, distribution, and molecular expression. In COPD lungs, these novel histopathological features were found to be correlated to lung function and they may thus have clinical consequences.},
  author       = {Andersson, Cecilia K and Mori, Michiko and Bjermer, Leif and Löfdahl, Claes-Göran and Erjefält, Jonas},
  issn         = {1535-4970},
  language     = {eng},
  number       = {3},
  pages        = {206--217},
  publisher    = {Am Thoracic Soc},
  series       = {American Journal of Respiratory and Critical Care Medicine},
  title        = {Alterations in Lung Mast Cell Populations in Patients with COPD.},
  url          = {http://dx.doi.org/10.1164/rccm.200906-0932OC},
  volume       = {181},
  year         = {2010},
}