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Integrated selective enrichment target - a microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases

Ekström, Simon LU ; Wallman, Lars LU ; Malm, Johan LU ; Becker, Charlotte LU ; Lilja, Hans LU ; Laurell, Thomas LU and Marko-Varga, György LU (2004) In Electrophoresis 25(21-22). p.3769-3777
Abstract
The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity... (More)
The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Electrophoresis
volume
25
issue
21-22
pages
3769 - 3777
publisher
John Wiley & Sons
external identifiers
  • wos:000225679000027
  • pmid:15565686
  • scopus:10944251786
ISSN
0173-0835
DOI
10.1002/elps.200406094
language
English
LU publication?
yes
id
05f2fc54-64e5-4602-be98-1f8c8e36e9e2 (old id 151176)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15565686&query_hl=15&itool=pubmed_docsum
date added to LUP
2007-06-26 14:54:26
date last changed
2017-09-10 03:45:56
@article{05f2fc54-64e5-4602-be98-1f8c8e36e9e2,
  abstract     = {The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples.},
  author       = {Ekström, Simon and Wallman, Lars and Malm, Johan and Becker, Charlotte and Lilja, Hans and Laurell, Thomas and Marko-Varga, György},
  issn         = {0173-0835},
  language     = {eng},
  number       = {21-22},
  pages        = {3769--3777},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Integrated selective enrichment target - a microtechnology platform for matrix-assisted laser desorption/ionization-mass spectrometry applied on protein biomarkers in prostate diseases},
  url          = {http://dx.doi.org/10.1002/elps.200406094},
  volume       = {25},
  year         = {2004},
}