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Multivariate data analysis of dynamic amperometric biosensor responses from binary analyte mixtures - application of sensitivity correction algorithms

Dock, Eva LU ; Christensen, J; Olsson, Mattias; Tønning, E; Ruzgas, Tautgirdas LU and Emnéus, Jenny LU (2005) In Talanta 65(2). p.298-305
Abstract
In this paper, it is demonstrated that a single-receptor biosensor can be used to quantitatively determine each analyte in binary Mixtures LIS in multivariate data analysis tools based on the dynamic responses received from flow injection peaks. Mixtures with different concentrations of two phenolic compounds, catechol and 4-chlorophenol, were measured with a graphite electrode modified with tyrosinase enzyme at an applied potential of -50 mV versus Ag/AgCl. A correction algorithm based on measurements of references in-between samples was applied to compensate for biosensor ageing as well as differences caused by deviations between biosensor preparations. After correction, the relative prediction errors with partial least squares... (More)
In this paper, it is demonstrated that a single-receptor biosensor can be used to quantitatively determine each analyte in binary Mixtures LIS in multivariate data analysis tools based on the dynamic responses received from flow injection peaks. Mixtures with different concentrations of two phenolic compounds, catechol and 4-chlorophenol, were measured with a graphite electrode modified with tyrosinase enzyme at an applied potential of -50 mV versus Ag/AgCl. A correction algorithm based on measurements of references in-between samples was applied to compensate for biosensor ageing as well as differences caused by deviations between biosensor preparations. After correction, the relative prediction errors with partial least squares regression (PLS-R) for catechol and 4-chlorophenol were 7.4 and 5.5%, respectively, using an analysis sequence measured on one biosensor. Additional validation mixtures of the two phenols were measured with a new biosensor, prepared with the same procedure but with a different batch of tyrosinase enzyme. Using the mixture responses for the first sensor as a calibration set in PLS-R. the relative prediction errors of the validation mixtures, after applying correction procedures. were 7.0% for catechol and 16.0% for 4-chlorophenol. These preliminary results indicate that by applying correction algorithms it could be possible to use less stable biosensors in continuous on-line measurements together with multivariate data analysis without time-consuming calibration procedures. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Talanta
volume
65
issue
2
pages
298 - 305
publisher
Elsevier
external identifiers
  • wos:000225381900002
  • scopus:8444227045
ISSN
1873-3573
DOI
10.1016/j.talanta.2004.07.002
language
English
LU publication?
yes
id
39bd35b2-46dc-46a0-aa82-b7ef44e1546e (old id 151185)
date added to LUP
2007-06-26 14:30:38
date last changed
2017-09-10 04:26:53
@article{39bd35b2-46dc-46a0-aa82-b7ef44e1546e,
  abstract     = {In this paper, it is demonstrated that a single-receptor biosensor can be used to quantitatively determine each analyte in binary Mixtures LIS in multivariate data analysis tools based on the dynamic responses received from flow injection peaks. Mixtures with different concentrations of two phenolic compounds, catechol and 4-chlorophenol, were measured with a graphite electrode modified with tyrosinase enzyme at an applied potential of -50 mV versus Ag/AgCl. A correction algorithm based on measurements of references in-between samples was applied to compensate for biosensor ageing as well as differences caused by deviations between biosensor preparations. After correction, the relative prediction errors with partial least squares regression (PLS-R) for catechol and 4-chlorophenol were 7.4 and 5.5%, respectively, using an analysis sequence measured on one biosensor. Additional validation mixtures of the two phenols were measured with a new biosensor, prepared with the same procedure but with a different batch of tyrosinase enzyme. Using the mixture responses for the first sensor as a calibration set in PLS-R. the relative prediction errors of the validation mixtures, after applying correction procedures. were 7.0% for catechol and 16.0% for 4-chlorophenol. These preliminary results indicate that by applying correction algorithms it could be possible to use less stable biosensors in continuous on-line measurements together with multivariate data analysis without time-consuming calibration procedures. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Dock, Eva and Christensen, J and Olsson, Mattias and Tønning, E and Ruzgas, Tautgirdas and Emnéus, Jenny},
  issn         = {1873-3573},
  language     = {eng},
  number       = {2},
  pages        = {298--305},
  publisher    = {Elsevier},
  series       = {Talanta},
  title        = {Multivariate data analysis of dynamic amperometric biosensor responses from binary analyte mixtures - application of sensitivity correction algorithms},
  url          = {http://dx.doi.org/10.1016/j.talanta.2004.07.002},
  volume       = {65},
  year         = {2005},
}