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Multienzyme electrochemical array sensor for determination of phenols and pesticides

Solna, R; Sapelnikova, Svetlana LU ; Skladal, P; Winther-Nielsen, M; Carlsson, C; Emnéus, Jenny LU and Ruzgas, Tautgirdas LU (2005) In Talanta 65(2). p.349-357
Abstract
The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase. peroxidase. acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition. the effects of heavy metals (CL2+, Cd2+, Fe3+), fluoride... (More)
The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase. peroxidase. acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition. the effects of heavy metals (CL2+, Cd2+, Fe3+), fluoride (NaF), benzene and dimethylsulphoxide on cholinesterase activities ere evaluated. It was demonstrated that electrodes modified with hydrolases and oxidoreductases can function in the same array. The achieved R.S.D. values obtained for the flow system were below 4% for the same sensor and less than 10% within a group of five sensors. For the steady state system, R.S.D.s were approximately twice higher. One assay was completed in less than 6 min. The limit of detection for catechol Using tyrosinase was equal to 0.35 and 1.7 muM in the flow and steady state systems, respectively. On the contrary, lower limits of detection for pesticides were achieved in the steady state system-carbaryl 26 W, heptenophos 14 nM and fenitrothion 0.58 muM. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Talanta
volume
65
issue
2
pages
349 - 357
publisher
Elsevier
external identifiers
  • wos:000225381900009
  • scopus:8444227043
ISSN
1873-3573
DOI
10.1016/j.talanta.2004.07.005
language
English
LU publication?
yes
id
686e2bfa-7a7f-47d5-99de-e33626ea3ec2 (old id 151193)
date added to LUP
2007-06-27 15:58:13
date last changed
2017-07-02 04:16:17
@article{686e2bfa-7a7f-47d5-99de-e33626ea3ec2,
  abstract     = {The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase. peroxidase. acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition. the effects of heavy metals (CL2+, Cd2+, Fe3+), fluoride (NaF), benzene and dimethylsulphoxide on cholinesterase activities ere evaluated. It was demonstrated that electrodes modified with hydrolases and oxidoreductases can function in the same array. The achieved R.S.D. values obtained for the flow system were below 4% for the same sensor and less than 10% within a group of five sensors. For the steady state system, R.S.D.s were approximately twice higher. One assay was completed in less than 6 min. The limit of detection for catechol Using tyrosinase was equal to 0.35 and 1.7 muM in the flow and steady state systems, respectively. On the contrary, lower limits of detection for pesticides were achieved in the steady state system-carbaryl 26 W, heptenophos 14 nM and fenitrothion 0.58 muM. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Solna, R and Sapelnikova, Svetlana and Skladal, P and Winther-Nielsen, M and Carlsson, C and Emnéus, Jenny and Ruzgas, Tautgirdas},
  issn         = {1873-3573},
  language     = {eng},
  number       = {2},
  pages        = {349--357},
  publisher    = {Elsevier},
  series       = {Talanta},
  title        = {Multienzyme electrochemical array sensor for determination of phenols and pesticides},
  url          = {http://dx.doi.org/10.1016/j.talanta.2004.07.005},
  volume       = {65},
  year         = {2005},
}