Multienzyme electrochemical array sensor for determination of phenols and pesticides
(2005) In Talanta 65(2). p.349-357- Abstract
- The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase. peroxidase. acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition. the effects of heavy metals (CL2+, Cd2+, Fe3+), fluoride... (More)
- The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase. peroxidase. acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition. the effects of heavy metals (CL2+, Cd2+, Fe3+), fluoride (NaF), benzene and dimethylsulphoxide on cholinesterase activities ere evaluated. It was demonstrated that electrodes modified with hydrolases and oxidoreductases can function in the same array. The achieved R.S.D. values obtained for the flow system were below 4% for the same sensor and less than 10% within a group of five sensors. For the steady state system, R.S.D.s were approximately twice higher. One assay was completed in less than 6 min. The limit of detection for catechol Using tyrosinase was equal to 0.35 and 1.7 muM in the flow and steady state systems, respectively. On the contrary, lower limits of detection for pesticides were achieved in the steady state system-carbaryl 26 W, heptenophos 14 nM and fenitrothion 0.58 muM. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/151193
- author
- Solna, R ; Sapelnikova, Svetlana LU ; Skladal, P ; Winther-Nielsen, M ; Carlsson, C ; Emnéus, Jenny LU and Ruzgas, Tautgirdas LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Talanta
- volume
- 65
- issue
- 2
- pages
- 349 - 357
- publisher
- Elsevier
- external identifiers
-
- wos:000225381900009
- scopus:8444227043
- ISSN
- 1873-3573
- DOI
- 10.1016/j.talanta.2004.07.005
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 686e2bfa-7a7f-47d5-99de-e33626ea3ec2 (old id 151193)
- date added to LUP
- 2016-04-01 15:34:05
- date last changed
- 2022-01-28 06:00:00
@article{686e2bfa-7a7f-47d5-99de-e33626ea3ec2,
abstract = {{The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase. peroxidase. acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition. the effects of heavy metals (CL2+, Cd2+, Fe3+), fluoride (NaF), benzene and dimethylsulphoxide on cholinesterase activities ere evaluated. It was demonstrated that electrodes modified with hydrolases and oxidoreductases can function in the same array. The achieved R.S.D. values obtained for the flow system were below 4% for the same sensor and less than 10% within a group of five sensors. For the steady state system, R.S.D.s were approximately twice higher. One assay was completed in less than 6 min. The limit of detection for catechol Using tyrosinase was equal to 0.35 and 1.7 muM in the flow and steady state systems, respectively. On the contrary, lower limits of detection for pesticides were achieved in the steady state system-carbaryl 26 W, heptenophos 14 nM and fenitrothion 0.58 muM. (C) 2004 Elsevier B.V. All rights reserved.}},
author = {{Solna, R and Sapelnikova, Svetlana and Skladal, P and Winther-Nielsen, M and Carlsson, C and Emnéus, Jenny and Ruzgas, Tautgirdas}},
issn = {{1873-3573}},
language = {{eng}},
number = {{2}},
pages = {{349--357}},
publisher = {{Elsevier}},
series = {{Talanta}},
title = {{Multienzyme electrochemical array sensor for determination of phenols and pesticides}},
url = {{http://dx.doi.org/10.1016/j.talanta.2004.07.005}},
doi = {{10.1016/j.talanta.2004.07.005}},
volume = {{65}},
year = {{2005}},
}