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Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers.

Coman, Vasile LU ; Gustavsson, Tobias LU ; Finkelsteinas, Arnonas LU ; von Wachenfeldt, Claes LU ; Hägerhäll, Cecilia LU and Gorton, Lo LU (2009) In Journal of the American Chemical Society 131(44). p.16171-16176
Abstract
The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical "wiring" of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as... (More)
The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical "wiring" of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as substrate, in both batch and flow analysis modes, and using gold and graphite electrodes. The efficiency of the osmium redox polymer, working as electron transfer mediator between the cells and the electrode, was compared with that of a soluble mediator (hexacyanoferrate). The results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the American Chemical Society
volume
131
issue
44
pages
16171 - 16176
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000271513700051
  • pmid:19886699
  • scopus:70450189500
  • pmid:19886699
ISSN
1520-5126
DOI
10.1021/ja905442a
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004), Molecular Cell Biology (432112241)
id
0185d138-a579-4e77-b05b-0e0a2db32293 (old id 1512283)
date added to LUP
2016-04-01 14:49:23
date last changed
2021-09-01 04:38:33
@article{0185d138-a579-4e77-b05b-0e0a2db32293,
  abstract     = {The present study explores genetic engineering of the respiratory chain and the application of two different flexible osmium redox polymers to achieve efficient electric communication between the gram-positive organism Bacillus subtilis and an electrode. Poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-bipyridyl)(2)Cl(2)](+/2+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for efficient electrical "wiring" of viable gram-positive bacterial cells to electrodes. Using a B. subtilis strain that overproduces succinate/quinone oxidoreductase (respiratory complex II), we were able to improve the current response several fold using succinate as substrate, in both batch and flow analysis modes, and using gold and graphite electrodes. The efficiency of the osmium redox polymer, working as electron transfer mediator between the cells and the electrode, was compared with that of a soluble mediator (hexacyanoferrate). The results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes.},
  author       = {Coman, Vasile and Gustavsson, Tobias and Finkelsteinas, Arnonas and von Wachenfeldt, Claes and Hägerhäll, Cecilia and Gorton, Lo},
  issn         = {1520-5126},
  language     = {eng},
  number       = {44},
  pages        = {16171--16176},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Journal of the American Chemical Society},
  title        = {Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers.},
  url          = {http://dx.doi.org/10.1021/ja905442a},
  doi          = {10.1021/ja905442a},
  volume       = {131},
  year         = {2009},
}