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Fibrocytes are associated with vascular and parenchymal remodelling in patients with obliterative bronchiolitis.

Andersson Sjöland, Annika LU ; Erjefält, Jonas LU ; Bjermer, Leif LU ; Eriksson, Leif LU and Westergren-Thorsson, Gunilla LU (2009) In Respiratory Research 10(Oct 30).
Abstract
BACKGROUND: The aim of the present study was to explore the occurrence of fibrocytes in tissue and to investigate whether the appearance of fibrocytes may be linked to structural changes of the parenchyme and vasculature in the lungs of patients with obliterative bronchiolitis (OB) following lung or bone marrow transplantation. METHODS: Identification of parenchyme, vasculature, and fibrocytes was done by histological methods in lung tissue from bone marrow or lung-transplanted patients with obliterative bronchiolitis, and from controls. RESULTS: The transplanted patients had significantly higher amounts of tissue in the alveolar parenchyme (46.5 +/- 17.6%) than the controls (21.7 +/- 7.6%) (p < 0.05). The patients also had... (More)
BACKGROUND: The aim of the present study was to explore the occurrence of fibrocytes in tissue and to investigate whether the appearance of fibrocytes may be linked to structural changes of the parenchyme and vasculature in the lungs of patients with obliterative bronchiolitis (OB) following lung or bone marrow transplantation. METHODS: Identification of parenchyme, vasculature, and fibrocytes was done by histological methods in lung tissue from bone marrow or lung-transplanted patients with obliterative bronchiolitis, and from controls. RESULTS: The transplanted patients had significantly higher amounts of tissue in the alveolar parenchyme (46.5 +/- 17.6%) than the controls (21.7 +/- 7.6%) (p < 0.05). The patients also had significantly increased numbers of fibrocytes identified by CXCR4/prolyl4-hydroxylase, CD45R0/prolyl4-hydroxylase, and CD34/prolyl4-hydroxylase compared to the controls (p < 0.01). There was a correlation between the number of fibrocytes and the area of alveolar parenchyma; CXCR4/prolyl 4-hydroxylase (p < 0.01), CD45R0/prolyl 4-hydroxylase (p < 0.05) and CD34/prolyl 4-hydroxylase (p < 0.05). In the pulmonary vessels, there was an increase in the endothelial layer in patients (0.31 +/- 0.13%) relative to the controls (0.037 +/- 0.02%) (p < 0.01). There was a significant correlation between the number of fibrocytes and the total area of the endothelial layer CXCR4/prolyl 4-hydroxylase (p < 0.001), CD45R0/prolyl 4-hydroxylase (p < 0.001) and CD34/prolyl 4-hydroxylase (p < 0.01). The percent areas of the lumen of the vessels were significant (p < 0.001) enlarged in the patient with OB compared to the controls. There was also a correlation between total area of the lumen and number of fibrocytes, CXCR4/prolyl 4-hydroxylase (p < 0.01), CD45R0/prolyl 4-hydroxylase (p < 0.001) and CD34/prolyl 4-hydroxylase (p < 0.01). CONCLUSION: Our results indicate that fibrocytes are associated with pathological remodelling processes in patients with OB and that tissue fibrocytes might be a useful biomarker in these processes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Respiratory Research
volume
10
issue
Oct 30
publisher
BioMed Central
external identifiers
  • wos:000271637900001
  • pmid:19878544
  • scopus:73949096804
ISSN
1465-9921
DOI
10.1186/1465-9921-10-103
language
English
LU publication?
yes
id
c097178a-f1e3-4b24-a44e-bb3dd0a6a663 (old id 1512426)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19878544?dopt=Abstract
date added to LUP
2009-12-01 10:51:04
date last changed
2017-08-06 04:46:25
@article{c097178a-f1e3-4b24-a44e-bb3dd0a6a663,
  abstract     = {BACKGROUND: The aim of the present study was to explore the occurrence of fibrocytes in tissue and to investigate whether the appearance of fibrocytes may be linked to structural changes of the parenchyme and vasculature in the lungs of patients with obliterative bronchiolitis (OB) following lung or bone marrow transplantation. METHODS: Identification of parenchyme, vasculature, and fibrocytes was done by histological methods in lung tissue from bone marrow or lung-transplanted patients with obliterative bronchiolitis, and from controls. RESULTS: The transplanted patients had significantly higher amounts of tissue in the alveolar parenchyme (46.5 +/- 17.6%) than the controls (21.7 +/- 7.6%) (p &lt; 0.05). The patients also had significantly increased numbers of fibrocytes identified by CXCR4/prolyl4-hydroxylase, CD45R0/prolyl4-hydroxylase, and CD34/prolyl4-hydroxylase compared to the controls (p &lt; 0.01). There was a correlation between the number of fibrocytes and the area of alveolar parenchyma; CXCR4/prolyl 4-hydroxylase (p &lt; 0.01), CD45R0/prolyl 4-hydroxylase (p &lt; 0.05) and CD34/prolyl 4-hydroxylase (p &lt; 0.05). In the pulmonary vessels, there was an increase in the endothelial layer in patients (0.31 +/- 0.13%) relative to the controls (0.037 +/- 0.02%) (p &lt; 0.01). There was a significant correlation between the number of fibrocytes and the total area of the endothelial layer CXCR4/prolyl 4-hydroxylase (p &lt; 0.001), CD45R0/prolyl 4-hydroxylase (p &lt; 0.001) and CD34/prolyl 4-hydroxylase (p &lt; 0.01). The percent areas of the lumen of the vessels were significant (p &lt; 0.001) enlarged in the patient with OB compared to the controls. There was also a correlation between total area of the lumen and number of fibrocytes, CXCR4/prolyl 4-hydroxylase (p &lt; 0.01), CD45R0/prolyl 4-hydroxylase (p &lt; 0.001) and CD34/prolyl 4-hydroxylase (p &lt; 0.01). CONCLUSION: Our results indicate that fibrocytes are associated with pathological remodelling processes in patients with OB and that tissue fibrocytes might be a useful biomarker in these processes.},
  articleno    = {103},
  author       = {Andersson Sjöland, Annika and Erjefält, Jonas and Bjermer, Leif and Eriksson, Leif and Westergren-Thorsson, Gunilla},
  issn         = {1465-9921},
  language     = {eng},
  number       = {Oct 30},
  publisher    = {BioMed Central},
  series       = {Respiratory Research},
  title        = {Fibrocytes are associated with vascular and parenchymal remodelling in patients with obliterative bronchiolitis.},
  url          = {http://dx.doi.org/10.1186/1465-9921-10-103},
  volume       = {10},
  year         = {2009},
}