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Improved chemical analysis of cellulose ethers using dialkylamine derivatization and mass spectrometry

Momcilovic, Dane LU ; Schagerlöf, Herje LU ; Wittgren, B; Wahlund, Karl-Gustav LU and Brinkmalm, G (2005) In Biomacromolecules 6(5). p.2793-2799
Abstract
Oligosaccharides of hydroxypropylmethyl cellulose, hydroxypropyl cellulose, and methyl cellulose were investigated by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The cellulose ether oligosaccharides were produced either by enzymatic depolymerization utilizing the purified family 5 endoglucanase from Bacillus agaradhaerens or by partial acidic depolymerization. To lower the limit of detection in MALDI-MS three dilakylamines, dimethyl-, diethyl-, and dipropylamine were studied as reagents for reductive amination of the oligosaccharides. All three amines contributed to a significant increase in sensitivity in MALDI-MS, especially for oligosaccharides with a degree of polymerization (DP) < 3. These reagents... (More)
Oligosaccharides of hydroxypropylmethyl cellulose, hydroxypropyl cellulose, and methyl cellulose were investigated by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The cellulose ether oligosaccharides were produced either by enzymatic depolymerization utilizing the purified family 5 endoglucanase from Bacillus agaradhaerens or by partial acidic depolymerization. To lower the limit of detection in MALDI-MS three dilakylamines, dimethyl-, diethyl-, and dipropylamine were studied as reagents for reductive amination of the oligosaccharides. All three amines contributed to a significant increase in sensitivity in MALDI-MS, especially for oligosaccharides with a degree of polymerization (DP) < 3. These reagents were also attractive due to their high volatility, which facilitated the purification of the reaction mixtures. It was established that low-mass discrimination in MALDI-MS in the DP range 1-7 was substantially reduced with dialkylamine derivatization. Hence, dialkylamine derivatization of cellulose ether oligosaccharides obtained by endoglucanase depolymerization increased the number of detected analyte components. Dimethylamine was concluded to be the preferred reagent of those evaluated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
6
issue
5
pages
2793 - 2799
publisher
The American Chemical Society
external identifiers
  • wos:000231899200051
  • scopus:25844500727
ISSN
1526-4602
DOI
10.1021/bm050270f
language
English
LU publication?
yes
id
58bd0a3c-1f60-4325-8246-a7dbcab71bb2 (old id 151434)
date added to LUP
2007-06-27 12:43:39
date last changed
2017-01-01 04:19:52
@article{58bd0a3c-1f60-4325-8246-a7dbcab71bb2,
  abstract     = {Oligosaccharides of hydroxypropylmethyl cellulose, hydroxypropyl cellulose, and methyl cellulose were investigated by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The cellulose ether oligosaccharides were produced either by enzymatic depolymerization utilizing the purified family 5 endoglucanase from Bacillus agaradhaerens or by partial acidic depolymerization. To lower the limit of detection in MALDI-MS three dilakylamines, dimethyl-, diethyl-, and dipropylamine were studied as reagents for reductive amination of the oligosaccharides. All three amines contributed to a significant increase in sensitivity in MALDI-MS, especially for oligosaccharides with a degree of polymerization (DP) &lt; 3. These reagents were also attractive due to their high volatility, which facilitated the purification of the reaction mixtures. It was established that low-mass discrimination in MALDI-MS in the DP range 1-7 was substantially reduced with dialkylamine derivatization. Hence, dialkylamine derivatization of cellulose ether oligosaccharides obtained by endoglucanase depolymerization increased the number of detected analyte components. Dimethylamine was concluded to be the preferred reagent of those evaluated.},
  author       = {Momcilovic, Dane and Schagerlöf, Herje and Wittgren, B and Wahlund, Karl-Gustav and Brinkmalm, G},
  issn         = {1526-4602},
  language     = {eng},
  number       = {5},
  pages        = {2793--2799},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Improved chemical analysis of cellulose ethers using dialkylamine derivatization and mass spectrometry},
  url          = {http://dx.doi.org/10.1021/bm050270f},
  volume       = {6},
  year         = {2005},
}