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Transport of a hyaluronan-binding protein in brain tissue

Kappler, Joachim; Hegener, Oliver; Baader, Stephan L.; Franken, Sebastian; Gieselmann, Volkmar; Haeberlein, Hanns and Rauch, Uwe LU (2009) In Matrix Biology 28(7). p.396-405
Abstract
Hyaluronan is an unsulfated linear glycosaminoglycan with the ability to nucleate extracellular matrices by the formation of aggregates with lecticans. These matrices are essential during development of the central nervous system. In the prospective white matter of the developing brain hyaluronan is organized into fiber-like structures according to confocal microscopy of fixed slices which may guide the migration of neural precursor cells [Baier, C., S.L Baader, J. Jankowski, V. Gieselmann, K. Schilling, U. Rauch, and J. Kappler. 2007. Hyaluronan is organized into fiber-like structures along migratory pathways in the developing mouse cerebellum. Matrix Biol. 26: 348-58]. By using plasmon surface resonance, microinjection into brain slices... (More)
Hyaluronan is an unsulfated linear glycosaminoglycan with the ability to nucleate extracellular matrices by the formation of aggregates with lecticans. These matrices are essential during development of the central nervous system. In the prospective white matter of the developing brain hyaluronan is organized into fiber-like structures according to confocal microscopy of fixed slices which may guide the migration of neural precursor cells [Baier, C., S.L Baader, J. Jankowski, V. Gieselmann, K. Schilling, U. Rauch, and J. Kappler. 2007. Hyaluronan is organized into fiber-like structures along migratory pathways in the developing mouse cerebellum. Matrix Biol. 26: 348-58]. By using plasmon surface resonance, microinjection into brain slices and fluorescence correlation spectroscopy, we show that the brain-specific lecticans bind to, but also dissociate rather rapidly from hyaluronan. After microinjection into native cerebellar slices a GFP-tagged hyaluronan-binding neurocan fragment was enriched at binding sites in the prospective white matter, which had a directional orientation and formed local stationary concentration gradients in areas where binding sites are abundant. Fluorescence correlation spectroscopy measurements at fixed brain slices revealed that fiber-bound neurocan-GFP was mobile with Dfiber(neurocan-GFP) = 4 x 10(-10) cm(2)/s. Therefore, we propose that hyaluronan-rich fibers in the prospective white matter of the developing mouse cerebellum can guide the diffusion of lecticans. Since lecticans bind a variety of growth and mobility factors, their guided diffusion may contribute to the transport of these polypeptides and to the formation of concentration gradients. This mechanism could serve to encode positional information during development. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
diffusion, Guided, Neurocan, Lectican, Extracellular matrix, Brain development
in
Matrix Biology
volume
28
issue
7
pages
396 - 405
publisher
Elsevier
external identifiers
  • wos:000272102000004
  • scopus:70350405327
ISSN
1569-1802
DOI
10.1016/j.matbio.2009.06.002
language
English
LU publication?
yes
id
4562dcaf-2386-42f5-aa4d-e54e1d18d0da (old id 1518245)
date added to LUP
2010-01-13 11:07:22
date last changed
2017-01-01 06:21:37
@article{4562dcaf-2386-42f5-aa4d-e54e1d18d0da,
  abstract     = {Hyaluronan is an unsulfated linear glycosaminoglycan with the ability to nucleate extracellular matrices by the formation of aggregates with lecticans. These matrices are essential during development of the central nervous system. In the prospective white matter of the developing brain hyaluronan is organized into fiber-like structures according to confocal microscopy of fixed slices which may guide the migration of neural precursor cells [Baier, C., S.L Baader, J. Jankowski, V. Gieselmann, K. Schilling, U. Rauch, and J. Kappler. 2007. Hyaluronan is organized into fiber-like structures along migratory pathways in the developing mouse cerebellum. Matrix Biol. 26: 348-58]. By using plasmon surface resonance, microinjection into brain slices and fluorescence correlation spectroscopy, we show that the brain-specific lecticans bind to, but also dissociate rather rapidly from hyaluronan. After microinjection into native cerebellar slices a GFP-tagged hyaluronan-binding neurocan fragment was enriched at binding sites in the prospective white matter, which had a directional orientation and formed local stationary concentration gradients in areas where binding sites are abundant. Fluorescence correlation spectroscopy measurements at fixed brain slices revealed that fiber-bound neurocan-GFP was mobile with Dfiber(neurocan-GFP) = 4 x 10(-10) cm(2)/s. Therefore, we propose that hyaluronan-rich fibers in the prospective white matter of the developing mouse cerebellum can guide the diffusion of lecticans. Since lecticans bind a variety of growth and mobility factors, their guided diffusion may contribute to the transport of these polypeptides and to the formation of concentration gradients. This mechanism could serve to encode positional information during development. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Kappler, Joachim and Hegener, Oliver and Baader, Stephan L. and Franken, Sebastian and Gieselmann, Volkmar and Haeberlein, Hanns and Rauch, Uwe},
  issn         = {1569-1802},
  keyword      = {diffusion,Guided,Neurocan,Lectican,Extracellular matrix,Brain development},
  language     = {eng},
  number       = {7},
  pages        = {396--405},
  publisher    = {Elsevier},
  series       = {Matrix Biology},
  title        = {Transport of a hyaluronan-binding protein in brain tissue},
  url          = {http://dx.doi.org/10.1016/j.matbio.2009.06.002},
  volume       = {28},
  year         = {2009},
}