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Splicing Variation at a FLOWERING LOCUS C Homeolog Is Associated With Flowering Time Variation in the Tetraploid Capsella bursa-pastoris

Slotte, Tanja; Huang, Hui-Run; Holm, Karl; Ceplitis, Alf LU ; Onge, Kate St.; Chen, Jun; Lagercrantz, Ulf and Lascoux, Martin (2009) In Genetics 183(1). p.337-345
Abstract
The long-term fates of duplicate genes are well studied both empirically and theoretically, but how the short-term evolution of duplicate genes contributes to phenotypic variation is less well known. Here, we have studied the genetic basis of flowering tithe variation in the disomic tetraploid Capsella bursa-pastoris. We sequenced four duplicate candidate genes for flowering time and 10 background loci in samples from western Eurasia and China. Using a mixed-model approach that accounts for population structure, we found that polymorphisms at one homeolog of two candidate genes, FLOWERING LOCUS C (FLC) and CRYPTOCHROME1 (CRY1), were associated with natural flowering time variation. No potentially causative polymorphisms were found in the... (More)
The long-term fates of duplicate genes are well studied both empirically and theoretically, but how the short-term evolution of duplicate genes contributes to phenotypic variation is less well known. Here, we have studied the genetic basis of flowering tithe variation in the disomic tetraploid Capsella bursa-pastoris. We sequenced four duplicate candidate genes for flowering time and 10 background loci in samples from western Eurasia and China. Using a mixed-model approach that accounts for population structure, we found that polymorphisms at one homeolog of two candidate genes, FLOWERING LOCUS C (FLC) and CRYPTOCHROME1 (CRY1), were associated with natural flowering time variation. No potentially causative polymorphisms were found in the coding region of CRY1; however, at FLC two splice site polymorphisms were associated with early flowering. Accessions harboring nonconsensus splice sites expressed an alternatively spliced transcript or did not express this FTC homeolog. Our results are consistent with the function of FLC as a major repressor of flowering in Arabidopsis thaliana and imply that non-functionalization of duplicate genes could provide an important source of phenotypic variation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Genetics
volume
183
issue
1
pages
337 - 345
publisher
Genetics Society of America
external identifiers
  • wos:000272067000030
  • scopus:70350170139
ISSN
0016-6731
DOI
10.1534/genetics.109.103705
language
English
LU publication?
yes
id
4c0294f9-6eff-4e1c-ab49-a2387a3e8e78 (old id 1518409)
date added to LUP
2010-01-07 15:55:17
date last changed
2017-07-23 03:50:58
@article{4c0294f9-6eff-4e1c-ab49-a2387a3e8e78,
  abstract     = {The long-term fates of duplicate genes are well studied both empirically and theoretically, but how the short-term evolution of duplicate genes contributes to phenotypic variation is less well known. Here, we have studied the genetic basis of flowering tithe variation in the disomic tetraploid Capsella bursa-pastoris. We sequenced four duplicate candidate genes for flowering time and 10 background loci in samples from western Eurasia and China. Using a mixed-model approach that accounts for population structure, we found that polymorphisms at one homeolog of two candidate genes, FLOWERING LOCUS C (FLC) and CRYPTOCHROME1 (CRY1), were associated with natural flowering time variation. No potentially causative polymorphisms were found in the coding region of CRY1; however, at FLC two splice site polymorphisms were associated with early flowering. Accessions harboring nonconsensus splice sites expressed an alternatively spliced transcript or did not express this FTC homeolog. Our results are consistent with the function of FLC as a major repressor of flowering in Arabidopsis thaliana and imply that non-functionalization of duplicate genes could provide an important source of phenotypic variation.},
  author       = {Slotte, Tanja and Huang, Hui-Run and Holm, Karl and Ceplitis, Alf and Onge, Kate St. and Chen, Jun and Lagercrantz, Ulf and Lascoux, Martin},
  issn         = {0016-6731},
  language     = {eng},
  number       = {1},
  pages        = {337--345},
  publisher    = {Genetics Society of America},
  series       = {Genetics},
  title        = {Splicing Variation at a FLOWERING LOCUS C Homeolog Is Associated With Flowering Time Variation in the Tetraploid Capsella bursa-pastoris},
  url          = {http://dx.doi.org/10.1534/genetics.109.103705},
  volume       = {183},
  year         = {2009},
}