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Improved bone-forming functionality on diameter-controlled TiO2 nanotube surface

Brammer, Karla S.; Oh, Seunghan; Cobb, Christine J.; Bjursten, Lars Magnus LU ; van der Heyde, Henri and Jin, Sungho (2009) In Acta Biomaterialia 5(8). p.3215-3223
Abstract
The titanium dioxide (TiO2) nanotube surface enables significantly accelerated osteoblast adhesion and exhibits strong bonding with bone. We prepared various sizes (30-100 nm diameter) of titanium dioxide (TiO2) nanotubes on titanium substrates by anodization and investigated the osteoblast cellular behavior in response to these different nanotube sizes. The unique and striking result of this study is that a change in osteoblast behavior is obtained in a relatively narrow range of nanotube dimensions, with small diameter (similar to 30 nm) nanotubes promoting the highest degree of osteoblast adhesion, while larger diameter (70-100 nm) nanotubes elicit a lower population of cells with extremely elongated cellular morphology and much higher... (More)
The titanium dioxide (TiO2) nanotube surface enables significantly accelerated osteoblast adhesion and exhibits strong bonding with bone. We prepared various sizes (30-100 nm diameter) of titanium dioxide (TiO2) nanotubes on titanium substrates by anodization and investigated the osteoblast cellular behavior in response to these different nanotube sizes. The unique and striking result of this study is that a change in osteoblast behavior is obtained in a relatively narrow range of nanotube dimensions, with small diameter (similar to 30 nm) nanotubes promoting the highest degree of osteoblast adhesion, while larger diameter (70-100 nm) nanotubes elicit a lower population of cells with extremely elongated cellular morphology and much higher alkaline phosphatase levels. Increased elongation of nuclei was also observed with larger diameter nanotubes. By controlling the nanotopography, large diameter nanotubes, in the similar to 100 min regime, induced extremely elongated cellular shapes, with an aspect ratio of 11:1, which resulted in substantially enhanced up-regulation of alkaline phosphatase activity, suggesting greater bone-forming ability than nanotubes with smaller diameters. Such nanotube structures, already being a strongly osseointegrating implant material, offer encouraging implications for the development and optimization of novel orthopedics-related treatments with precise control toward desired cell and bone growth behavior. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alkaline, phosphatase activity, Cell elongation, Cell adhesion, TiO2 nanotubes, Osteoblast
in
Acta Biomaterialia
volume
5
issue
8
pages
3215 - 3223
publisher
Elsevier
external identifiers
  • wos:000271389700039
  • scopus:70349146591
ISSN
1878-7568
DOI
10.1016/j.actbio.2009.05.008
language
English
LU publication?
yes
id
56d19313-d93c-4f21-986f-cba299cc1c51 (old id 1520843)
date added to LUP
2009-12-28 09:05:00
date last changed
2017-12-10 03:43:47
@article{56d19313-d93c-4f21-986f-cba299cc1c51,
  abstract     = {The titanium dioxide (TiO2) nanotube surface enables significantly accelerated osteoblast adhesion and exhibits strong bonding with bone. We prepared various sizes (30-100 nm diameter) of titanium dioxide (TiO2) nanotubes on titanium substrates by anodization and investigated the osteoblast cellular behavior in response to these different nanotube sizes. The unique and striking result of this study is that a change in osteoblast behavior is obtained in a relatively narrow range of nanotube dimensions, with small diameter (similar to 30 nm) nanotubes promoting the highest degree of osteoblast adhesion, while larger diameter (70-100 nm) nanotubes elicit a lower population of cells with extremely elongated cellular morphology and much higher alkaline phosphatase levels. Increased elongation of nuclei was also observed with larger diameter nanotubes. By controlling the nanotopography, large diameter nanotubes, in the similar to 100 min regime, induced extremely elongated cellular shapes, with an aspect ratio of 11:1, which resulted in substantially enhanced up-regulation of alkaline phosphatase activity, suggesting greater bone-forming ability than nanotubes with smaller diameters. Such nanotube structures, already being a strongly osseointegrating implant material, offer encouraging implications for the development and optimization of novel orthopedics-related treatments with precise control toward desired cell and bone growth behavior. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
  author       = {Brammer, Karla S. and Oh, Seunghan and Cobb, Christine J. and Bjursten, Lars Magnus and van der Heyde, Henri and Jin, Sungho},
  issn         = {1878-7568},
  keyword      = {Alkaline,phosphatase activity,Cell elongation,Cell adhesion,TiO2 nanotubes,Osteoblast},
  language     = {eng},
  number       = {8},
  pages        = {3215--3223},
  publisher    = {Elsevier},
  series       = {Acta Biomaterialia},
  title        = {Improved bone-forming functionality on diameter-controlled TiO2 nanotube surface},
  url          = {http://dx.doi.org/10.1016/j.actbio.2009.05.008},
  volume       = {5},
  year         = {2009},
}