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Genome-wide comparison of phage M13-infected vs. uninfected Escherichia coli

Karlsson, Fredrik LU ; Malmborg Hager, Ann-Christin LU ; Albrekt, Ann-Sofie LU and Borrebaeck, Carl LU (2005) In Canadian Journal of Microbiology 51(1). p.29-35
Abstract
To identify Escherichia coli genes potentially regulated by filamentous phage infection, we used oligonucleotide microarrays. Genome-wide comparison of phage M13-infected and uninfected E. coli, 2 and 20 min after infection, was performed. The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated with the known in vivo pattern of M13 mRNA species. Ten of the 12 host genes affected could be grouped into 3 different categories based on cellular function, suggesting a coordinated response. The significantly upregulated genes encode proteins involved in reactions of the energy-generating phosphotransferase system and transcription processing,... (More)
To identify Escherichia coli genes potentially regulated by filamentous phage infection, we used oligonucleotide microarrays. Genome-wide comparison of phage M13-infected and uninfected E. coli, 2 and 20 min after infection, was performed. The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated with the known in vivo pattern of M13 mRNA species. Ten of the 12 host genes affected could be grouped into 3 different categories based on cellular function, suggesting a coordinated response. The significantly upregulated genes encode proteins involved in reactions of the energy-generating phosphotransferase system and transcription processing, which could be related to phage transcription. No genes belonging to any known E. coli stress response pathways were scored as upregulated. Furthermore, phage infection led to significant downregulation of transcripts of the bacterial genes gadA, gadB, hdeA, gadE, slp, and crl. These downregulated genes are normally part of the host stress response mechanisms that protect the bacterium during conditions of acid stress and stationary phase transition. The phage-infected cells demonstrated impaired function of the oxidative and the glutamate-dependent acid resistance systems. Thus, global transcriptional analysis and functional analysis revealed previously unknown host responses to filamentous phage infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Canadian Journal of Microbiology
volume
51
issue
1
pages
29 - 35
publisher
National Research Council Canada
external identifiers
  • wos:000227840000005
  • pmid:15782232
  • scopus:19044393414
ISSN
0008-4166
DOI
10.1139/W04-113
language
English
LU publication?
yes
id
02849c9e-bd9e-432b-9cb1-76143244b15a (old id 152155)
date added to LUP
2007-07-06 12:25:49
date last changed
2017-01-08 05:01:59
@article{02849c9e-bd9e-432b-9cb1-76143244b15a,
  abstract     = {To identify Escherichia coli genes potentially regulated by filamentous phage infection, we used oligonucleotide microarrays. Genome-wide comparison of phage M13-infected and uninfected E. coli, 2 and 20 min after infection, was performed. The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated with the known in vivo pattern of M13 mRNA species. Ten of the 12 host genes affected could be grouped into 3 different categories based on cellular function, suggesting a coordinated response. The significantly upregulated genes encode proteins involved in reactions of the energy-generating phosphotransferase system and transcription processing, which could be related to phage transcription. No genes belonging to any known E. coli stress response pathways were scored as upregulated. Furthermore, phage infection led to significant downregulation of transcripts of the bacterial genes gadA, gadB, hdeA, gadE, slp, and crl. These downregulated genes are normally part of the host stress response mechanisms that protect the bacterium during conditions of acid stress and stationary phase transition. The phage-infected cells demonstrated impaired function of the oxidative and the glutamate-dependent acid resistance systems. Thus, global transcriptional analysis and functional analysis revealed previously unknown host responses to filamentous phage infection.},
  author       = {Karlsson, Fredrik and Malmborg Hager, Ann-Christin and Albrekt, Ann-Sofie and Borrebaeck, Carl},
  issn         = {0008-4166},
  language     = {eng},
  number       = {1},
  pages        = {29--35},
  publisher    = {National Research Council Canada},
  series       = {Canadian Journal of Microbiology},
  title        = {Genome-wide comparison of phage M13-infected vs. uninfected Escherichia coli},
  url          = {http://dx.doi.org/10.1139/W04-113},
  volume       = {51},
  year         = {2005},
}