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Cell guidance by magnetic nanowires.

Johansson, Fredrik LU ; Jonsson, Malin; Alm, Kersti LU and Kanje, Martin LU (2010) In Experimental Cell Research 316. p.688-694
Abstract
The phenomenon of contact guidance on thin fibers has been known since the beginning of the 20th century when Harrison studied cells growing on fibers from spider's web. Since then many studies have been performed on structured surfaces and fibers. Here we present a new way to induce guidance of cells or cell processes using magnetic nanowires. We have manufactured magnetic Ni-nanowires (200 nm in diameter and 40 mum long) with a template-based electro-deposition method. Drops of a nanowire/ethanol suspension were placed on glass cover slips. The nanowires were aligned in an external magnetic field and adhered to the cover slips after evaporation of the ethanol. When the wires had adhered, the magnetic field was removed. L929 fibroblasts... (More)
The phenomenon of contact guidance on thin fibers has been known since the beginning of the 20th century when Harrison studied cells growing on fibers from spider's web. Since then many studies have been performed on structured surfaces and fibers. Here we present a new way to induce guidance of cells or cell processes using magnetic nanowires. We have manufactured magnetic Ni-nanowires (200 nm in diameter and 40 mum long) with a template-based electro-deposition method. Drops of a nanowire/ethanol suspension were placed on glass cover slips. The nanowires were aligned in an external magnetic field and adhered to the cover slips after evaporation of the ethanol. When the wires had adhered, the magnetic field was removed. L929 fibroblasts and dissociated dorsal root ganglia (DRG) neurons from mice were cultured on the nanowire-coated cover slips for 24 h and 72 h respectively. The fibroblasts were affected by the aligned nanowires and displayed contact guidance. Regenerated axons also displayed contact guidance on the wires. There were no overt signs of toxicity caused by Ni-wires. Aligned magnetic nanowires can be useful for lab-on-a-chip devices and medical nerve grafts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Cell Research
volume
316
pages
688 - 694
publisher
Academic Press
external identifiers
  • wos:000275364300002
  • scopus:76749104226
ISSN
1090-2422
DOI
10.1016/j.yexcr.2009.12.016
language
English
LU publication?
yes
id
d73ac2e2-9612-48ef-919f-aa6b4bf2c542 (old id 1523262)
date added to LUP
2010-01-12 08:11:10
date last changed
2018-07-15 03:07:13
@article{d73ac2e2-9612-48ef-919f-aa6b4bf2c542,
  abstract     = {The phenomenon of contact guidance on thin fibers has been known since the beginning of the 20th century when Harrison studied cells growing on fibers from spider's web. Since then many studies have been performed on structured surfaces and fibers. Here we present a new way to induce guidance of cells or cell processes using magnetic nanowires. We have manufactured magnetic Ni-nanowires (200 nm in diameter and 40 mum long) with a template-based electro-deposition method. Drops of a nanowire/ethanol suspension were placed on glass cover slips. The nanowires were aligned in an external magnetic field and adhered to the cover slips after evaporation of the ethanol. When the wires had adhered, the magnetic field was removed. L929 fibroblasts and dissociated dorsal root ganglia (DRG) neurons from mice were cultured on the nanowire-coated cover slips for 24 h and 72 h respectively. The fibroblasts were affected by the aligned nanowires and displayed contact guidance. Regenerated axons also displayed contact guidance on the wires. There were no overt signs of toxicity caused by Ni-wires. Aligned magnetic nanowires can be useful for lab-on-a-chip devices and medical nerve grafts.},
  author       = {Johansson, Fredrik and Jonsson, Malin and Alm, Kersti and Kanje, Martin},
  issn         = {1090-2422},
  language     = {eng},
  pages        = {688--694},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {Cell guidance by magnetic nanowires.},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2009.12.016},
  volume       = {316},
  year         = {2010},
}