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RNA and DNA interactions with zwitterionic and charged lipid membranes - A DSC and QCM-D study.

Michanek, Agnes; Kristen, Nora LU ; Höök, Fredrik; Nylander, Tommy LU and Sparr, Emma LU (2010) In Biochimica et Biophysica Acta - Biomembranes 1798(4). p.829-838
Abstract
The aim of the present study is to establish under which conditions tRNA associate with phospholipid bilayers, and to explore how this interaction influence the lipid bilayer. For this purpose we have studied association of tRNA or DNA of different size and degree of base pairing with a set of model membrane systems with varying charge density, composed of zwitterionic phosphatidylcholines (PC) in mixtures with anionic phosphatidylserine (PS) or cationic dioctadecyl-dimethyl-ammoniumbromide (DODAB), and with fluid or solid acyl-chains (oleoyl, myristoyl and palmitoyl). To prove and quantify the attractive interaction between tRNA and model-lipid membrane we used quartz crystal microbalance with dissipation (QCM-D) monitoring to study the... (More)
The aim of the present study is to establish under which conditions tRNA associate with phospholipid bilayers, and to explore how this interaction influence the lipid bilayer. For this purpose we have studied association of tRNA or DNA of different size and degree of base pairing with a set of model membrane systems with varying charge density, composed of zwitterionic phosphatidylcholines (PC) in mixtures with anionic phosphatidylserine (PS) or cationic dioctadecyl-dimethyl-ammoniumbromide (DODAB), and with fluid or solid acyl-chains (oleoyl, myristoyl and palmitoyl). To prove and quantify the attractive interaction between tRNA and model-lipid membrane we used quartz crystal microbalance with dissipation (QCM-D) monitoring to study the tRNA adsorption to deposited phospholipid bilayers from solutions containing monovalent (Na(+)) or divalent (Ca(2+)) cations. The influence of the adsorbed polynucleic acids on the lipid phase transitions and lipid segregation was studied by means of differential scanning calorimetry (DSC). The basic findings are: i) tRNA adsorb to zwitterionic liquid-crystalline and gel-phase phospholipid bilayers. The interaction is weak and reversible, and cannot be explained only on basis of electrostatic attraction. ii) The adsorbed amount tRNA is higher for liquid-crystalline bilayers compared to gel-phase bilayers, while the presence of divalent cations show no significant effect on the tRNA adsorption. iii) The adsorption of tRNA can lead to segregation in the mixed 1,2-dimyristoyl-sn- glycerol-3-phosphatidylcholine (DMPC)- 1,2-dimyristoyl-sn-glycero-3- phosphatidylserine (DMPS) and DMPC-DODAB bilayers, where tRNA is likely excluded from the anionic DMPS-rich domains in the first system, and associated with the cationic DODAB-rich domains in the second system. iv) The addition of shorter polynucleic acids influence the chain melting transition and induce segregation in mixed DMPC-DMPS system, while larger polynucleic acids do not influence the melting transition in these system. The results in this study on tRNA-phospholipid interactions can have implications for its biological function in, e.g., the cell nuclei, as well as in applications in biotechnology and medicine. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
QCM-D, DSC, RNA, DNA, Phospholipids, Phospholipid phase behavior, Polynucleic acid adsorption
in
Biochimica et Biophysica Acta - Biomembranes
volume
1798
issue
4
pages
829 - 838
publisher
Elsevier
external identifiers
  • wos:000275998300011
  • pmid:20036213
  • scopus:77449103706
ISSN
0005-2736
DOI
10.1016/j.bbamem.2009.12.009
language
English
LU publication?
yes
id
6d71bf36-a475-41bd-84ec-8b2de5e4adb5 (old id 1523269)
alternative location
http://www.sciencedirect.com/science/article/B6T1T-4Y1NV62-1/2/1d430651b4588f13e78c2e771abfb56b
date added to LUP
2010-01-12 08:15:07
date last changed
2018-07-01 03:56:31
@article{6d71bf36-a475-41bd-84ec-8b2de5e4adb5,
  abstract     = {The aim of the present study is to establish under which conditions tRNA associate with phospholipid bilayers, and to explore how this interaction influence the lipid bilayer. For this purpose we have studied association of tRNA or DNA of different size and degree of base pairing with a set of model membrane systems with varying charge density, composed of zwitterionic phosphatidylcholines (PC) in mixtures with anionic phosphatidylserine (PS) or cationic dioctadecyl-dimethyl-ammoniumbromide (DODAB), and with fluid or solid acyl-chains (oleoyl, myristoyl and palmitoyl). To prove and quantify the attractive interaction between tRNA and model-lipid membrane we used quartz crystal microbalance with dissipation (QCM-D) monitoring to study the tRNA adsorption to deposited phospholipid bilayers from solutions containing monovalent (Na(+)) or divalent (Ca(2+)) cations. The influence of the adsorbed polynucleic acids on the lipid phase transitions and lipid segregation was studied by means of differential scanning calorimetry (DSC). The basic findings are: i) tRNA adsorb to zwitterionic liquid-crystalline and gel-phase phospholipid bilayers. The interaction is weak and reversible, and cannot be explained only on basis of electrostatic attraction. ii) The adsorbed amount tRNA is higher for liquid-crystalline bilayers compared to gel-phase bilayers, while the presence of divalent cations show no significant effect on the tRNA adsorption. iii) The adsorption of tRNA can lead to segregation in the mixed 1,2-dimyristoyl-sn- glycerol-3-phosphatidylcholine (DMPC)- 1,2-dimyristoyl-sn-glycero-3- phosphatidylserine (DMPS) and DMPC-DODAB bilayers, where tRNA is likely excluded from the anionic DMPS-rich domains in the first system, and associated with the cationic DODAB-rich domains in the second system. iv) The addition of shorter polynucleic acids influence the chain melting transition and induce segregation in mixed DMPC-DMPS system, while larger polynucleic acids do not influence the melting transition in these system. The results in this study on tRNA-phospholipid interactions can have implications for its biological function in, e.g., the cell nuclei, as well as in applications in biotechnology and medicine.},
  author       = {Michanek, Agnes and Kristen, Nora and Höök, Fredrik and Nylander, Tommy and Sparr, Emma},
  issn         = {0005-2736},
  keyword      = {QCM-D,DSC,RNA,DNA,Phospholipids,Phospholipid phase behavior,Polynucleic acid adsorption},
  language     = {eng},
  number       = {4},
  pages        = {829--838},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Biomembranes},
  title        = {RNA and DNA interactions with zwitterionic and charged lipid membranes - A DSC and QCM-D study.},
  url          = {http://dx.doi.org/10.1016/j.bbamem.2009.12.009},
  volume       = {1798},
  year         = {2010},
}