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Characterization of the antennal olfactory system of the bed bug (Cimex lectularius)

Harraca, Vincent LU ; Ignell, Rickard; Löfstedt, Christer LU and Ryne, Camilla LU (2010) In Chemical Senses 35. p.195-204
Abstract
The common bed bug Cimex lectularius (Hemiptera; Cimicidae) is a temporary ectoparasite on humans that is currently reinvading the developed countries. Like other haematophagous arthropods, host seeking and orientation in C. lectularius is partially mediated by olfaction. In this study, we reconfirmed the distribution of the 44 olfactory sensilla and identified 3 different sensillum types located at the distal tip of C. lectularius antenna by external morphology mapping. Using a panel of relevant odorants previously reported to be bioactive in various haematophagous arthropods, we correlated the morphological mapping with an electrophysiological characterization of the olfactory receptor neurons housed in each specific sensillum. We found... (More)
The common bed bug Cimex lectularius (Hemiptera; Cimicidae) is a temporary ectoparasite on humans that is currently reinvading the developed countries. Like other haematophagous arthropods, host seeking and orientation in C. lectularius is partially mediated by olfaction. In this study, we reconfirmed the distribution of the 44 olfactory sensilla and identified 3 different sensillum types located at the distal tip of C. lectularius antenna by external morphology mapping. Using a panel of relevant odorants previously reported to be bioactive in various haematophagous arthropods, we correlated the morphological mapping with an electrophysiological characterization of the olfactory receptor neurons housed in each specific sensillum. We found that all 9 grooved peg sensilla responded specifically in a dose-dependent manner to ammonia, whereas (E)-2-hexenal, (E)-2-octenal, dimethyl trisulfide, 6-methyl-5-hepten-2-one, alpha-pinene, indole, and ethyl butyrate evoked dose-dependent responses within the 6 smooth peg sensilla. Based on the pattern of response to the tested compounds, we were able to separate the 6 smooth peg sensilla of the bed bug into 3 distinct functional classes. We compare our results with previous electrophysiological recordings made with these compounds on other haematophagous arthropods. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemical Senses
volume
35
pages
195 - 204
publisher
Oxford University Press
external identifiers
  • wos:000274586800003
  • scopus:77950615156
ISSN
1464-3553
DOI
10.1093/chemse/bjp096
language
English
LU publication?
yes
id
3f5734b0-000f-45e5-9a8e-4f619cd4b424 (old id 1523363)
date added to LUP
2010-01-11 13:27:38
date last changed
2018-05-29 09:43:53
@article{3f5734b0-000f-45e5-9a8e-4f619cd4b424,
  abstract     = {The common bed bug Cimex lectularius (Hemiptera; Cimicidae) is a temporary ectoparasite on humans that is currently reinvading the developed countries. Like other haematophagous arthropods, host seeking and orientation in C. lectularius is partially mediated by olfaction. In this study, we reconfirmed the distribution of the 44 olfactory sensilla and identified 3 different sensillum types located at the distal tip of C. lectularius antenna by external morphology mapping. Using a panel of relevant odorants previously reported to be bioactive in various haematophagous arthropods, we correlated the morphological mapping with an electrophysiological characterization of the olfactory receptor neurons housed in each specific sensillum. We found that all 9 grooved peg sensilla responded specifically in a dose-dependent manner to ammonia, whereas (E)-2-hexenal, (E)-2-octenal, dimethyl trisulfide, 6-methyl-5-hepten-2-one, alpha-pinene, indole, and ethyl butyrate evoked dose-dependent responses within the 6 smooth peg sensilla. Based on the pattern of response to the tested compounds, we were able to separate the 6 smooth peg sensilla of the bed bug into 3 distinct functional classes. We compare our results with previous electrophysiological recordings made with these compounds on other haematophagous arthropods.},
  author       = {Harraca, Vincent and Ignell, Rickard and Löfstedt, Christer and Ryne, Camilla},
  issn         = {1464-3553},
  language     = {eng},
  pages        = {195--204},
  publisher    = {Oxford University Press},
  series       = {Chemical Senses},
  title        = {Characterization of the antennal olfactory system of the bed bug <i>(Cimex lectularius)</i>},
  url          = {http://dx.doi.org/10.1093/chemse/bjp096},
  volume       = {35},
  year         = {2010},
}