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The human IFN-inducible p53 target gene TRIM22 colocalizes with the centrosome independently of cell cycle phase.

Petersson, Jessica LU ; Lönnbro, Per LU ; Herr, Anna-Maria; Mörgelin, Matthias LU ; Gullberg, Urban LU and Drott, Kristina LU (2010) In Experimental Cell Research 316. p.568-579
Abstract
TRIM22 (Staf50), a member of the TRIM protein family, is an interferon (IFN)-inducible protein as well as a p53 target gene. The function of TRIM22 is largely unknown, but TRIM22 is suggested to play a role in viral defense by restriction of viral replication. In addition, TRIM22 may function as a ubiquitin E3 ligase. In contrast to previous reports showing solely cytoplasmic localization of exogenous TRIM22, we report here that endogenous TRIM22 is localized to both nucleus and cytosol in primary human mononuclear cells, as well as in the human osteosarcoma cell line U2OS. Moreover, we demonstrate a colocalization of TRIM22 with the centrosomes in primary cells as well as in U2OS cells, and show that this colocalization is independent of... (More)
TRIM22 (Staf50), a member of the TRIM protein family, is an interferon (IFN)-inducible protein as well as a p53 target gene. The function of TRIM22 is largely unknown, but TRIM22 is suggested to play a role in viral defense by restriction of viral replication. In addition, TRIM22 may function as a ubiquitin E3 ligase. In contrast to previous reports showing solely cytoplasmic localization of exogenous TRIM22, we report here that endogenous TRIM22 is localized to both nucleus and cytosol in primary human mononuclear cells, as well as in the human osteosarcoma cell line U2OS. Moreover, we demonstrate a colocalization of TRIM22 with the centrosomes in primary cells as well as in U2OS cells, and show that this colocalization is independent of cell cycle phase. Additionally, our data suggest the colocalization with centrosomes to be independent on the microtubule network. Given that some viral protein assembly takes place in the close vicinity of the centrosome, our data suggest that important functions of TRIM22 such as regulation of viral replication and protein degradation may take place in the centrosome. However, further studies are warranted to certify this notion. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Cell Research
volume
316
pages
568 - 579
publisher
Academic Press
external identifiers
  • wos:000274321100006
  • pmid:20006605
  • scopus:77449126563
ISSN
1090-2422
DOI
10.1016/j.yexcr.2009.12.007
language
English
LU publication?
yes
id
2b226f9d-6cb9-40b5-87c2-08582802f2e7 (old id 1523631)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20006605?dopt=Abstract
date added to LUP
2010-01-14 13:44:46
date last changed
2018-05-29 10:28:24
@article{2b226f9d-6cb9-40b5-87c2-08582802f2e7,
  abstract     = {TRIM22 (Staf50), a member of the TRIM protein family, is an interferon (IFN)-inducible protein as well as a p53 target gene. The function of TRIM22 is largely unknown, but TRIM22 is suggested to play a role in viral defense by restriction of viral replication. In addition, TRIM22 may function as a ubiquitin E3 ligase. In contrast to previous reports showing solely cytoplasmic localization of exogenous TRIM22, we report here that endogenous TRIM22 is localized to both nucleus and cytosol in primary human mononuclear cells, as well as in the human osteosarcoma cell line U2OS. Moreover, we demonstrate a colocalization of TRIM22 with the centrosomes in primary cells as well as in U2OS cells, and show that this colocalization is independent of cell cycle phase. Additionally, our data suggest the colocalization with centrosomes to be independent on the microtubule network. Given that some viral protein assembly takes place in the close vicinity of the centrosome, our data suggest that important functions of TRIM22 such as regulation of viral replication and protein degradation may take place in the centrosome. However, further studies are warranted to certify this notion.},
  author       = {Petersson, Jessica and Lönnbro, Per and Herr, Anna-Maria and Mörgelin, Matthias and Gullberg, Urban and Drott, Kristina},
  issn         = {1090-2422},
  language     = {eng},
  pages        = {568--579},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {The human IFN-inducible p53 target gene TRIM22 colocalizes with the centrosome independently of cell cycle phase.},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2009.12.007},
  volume       = {316},
  year         = {2010},
}