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Comparative sequence analysis of the non-protein-coding mitochondrial DNA of inbred rat strains.

Abhyankar, Avinash LU ; Park, Hee-Bok LU ; Tonolo, Giancarlo LU and Luthman, Holger LU (2009) In PLoS One 4(12).
Abstract
The proper function of mammalian mitochondria necessitates a coordinated expression of both nuclear and mitochondrial genes, most likely due to the co-evolution of nuclear and mitochondrial genomes. The non-protein coding regions of mitochondrial DNA (mtDNA) including the D-loop, tRNA and rRNA genes form a major component of this regulated expression unit. Here we present comparative analyses of the non-protein-coding regions from 27 Rattus norvegicus mtDNA sequences. There were two variable positions in 12S rRNA, 20 in 16S rRNA, eight within the tRNA genes and 13 in the D-loop. Only one of the three neutrality tests used demonstrated statistically significant evidence for selection in 16S rRNA and tRNA-Cys. Based on our analyses of... (More)
The proper function of mammalian mitochondria necessitates a coordinated expression of both nuclear and mitochondrial genes, most likely due to the co-evolution of nuclear and mitochondrial genomes. The non-protein coding regions of mitochondrial DNA (mtDNA) including the D-loop, tRNA and rRNA genes form a major component of this regulated expression unit. Here we present comparative analyses of the non-protein-coding regions from 27 Rattus norvegicus mtDNA sequences. There were two variable positions in 12S rRNA, 20 in 16S rRNA, eight within the tRNA genes and 13 in the D-loop. Only one of the three neutrality tests used demonstrated statistically significant evidence for selection in 16S rRNA and tRNA-Cys. Based on our analyses of conserved sequences, we propose that some of the variable nucleotide positions identified in 16S rRNA and tRNA-Cys, and the D-loop might be important for mitochondrial function and its regulation. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
PLoS One
volume
4
issue
12
publisher
Public Library of Science
external identifiers
  • WOS:000272829500010
  • PMID:19997590
  • Scopus:77949539305
ISSN
1932-6203
DOI
10.1371/journal.pone.0008148
language
English
LU publication?
yes
id
4324cb97-11ba-45af-8a6e-3300b47c52e8 (old id 1523736)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19997590?dopt=Abstract
date added to LUP
2010-01-14 10:05:01
date last changed
2017-01-15 04:27:59
@article{4324cb97-11ba-45af-8a6e-3300b47c52e8,
  abstract     = {The proper function of mammalian mitochondria necessitates a coordinated expression of both nuclear and mitochondrial genes, most likely due to the co-evolution of nuclear and mitochondrial genomes. The non-protein coding regions of mitochondrial DNA (mtDNA) including the D-loop, tRNA and rRNA genes form a major component of this regulated expression unit. Here we present comparative analyses of the non-protein-coding regions from 27 Rattus norvegicus mtDNA sequences. There were two variable positions in 12S rRNA, 20 in 16S rRNA, eight within the tRNA genes and 13 in the D-loop. Only one of the three neutrality tests used demonstrated statistically significant evidence for selection in 16S rRNA and tRNA-Cys. Based on our analyses of conserved sequences, we propose that some of the variable nucleotide positions identified in 16S rRNA and tRNA-Cys, and the D-loop might be important for mitochondrial function and its regulation.},
  articleno    = {e8148},
  author       = {Abhyankar, Avinash and Park, Hee-Bok and Tonolo, Giancarlo and Luthman, Holger},
  issn         = {1932-6203},
  language     = {eng},
  number       = {12},
  publisher    = {Public Library of Science},
  series       = {PLoS One},
  title        = {Comparative sequence analysis of the non-protein-coding mitochondrial DNA of inbred rat strains.},
  url          = {http://dx.doi.org/10.1371/journal.pone.0008148},
  volume       = {4},
  year         = {2009},
}