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Analysis of protein expression in pure cell nuclei populations isolated from human breast cancer tissue by DNA flow cytometric sorting.

Baldetorp, Bo LU ; Johansson, Maria C LU ; Ingvar, Christian LU ; Lundgren, Lotta LU ; Olsson, Håkan LU ; Jansson, Bo LU and Welinder, Charlotte LU (2010) In Journal of Proteomics2008-01-01+01:00 73. p.1111-1116
Abstract
In this study, cell nuclei from aneuploid breast cancer samples were sorted with respect to DNA content into pure diploid and aneuploid fractions using flow cytometry. The nuclear proteins were then separated by one-dimensional gel electrophoresis (1D-PAGE) and differences in protein expression patterns, between diploid and aneuploid nuclei from the same tumours, were compared. Using a combination of peptide finger printing and peptide identification by MALDI-TOF mass spectrometry, we identified proteins and confirmed that the proteins were of nuclear origins. The results in this study add further information to the knowledge about the breast cancer disease complexity and heterogeneity at molecular level. For some of the tumours studied... (More)
In this study, cell nuclei from aneuploid breast cancer samples were sorted with respect to DNA content into pure diploid and aneuploid fractions using flow cytometry. The nuclear proteins were then separated by one-dimensional gel electrophoresis (1D-PAGE) and differences in protein expression patterns, between diploid and aneuploid nuclei from the same tumours, were compared. Using a combination of peptide finger printing and peptide identification by MALDI-TOF mass spectrometry, we identified proteins and confirmed that the proteins were of nuclear origins. The results in this study add further information to the knowledge about the breast cancer disease complexity and heterogeneity at molecular level. For some of the tumours studied different nuclei protein patterns were obtained, in the diploid respective aneuploid nuclei populations, whilst other tumours did not show these differences. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteomics2008-01-01+01:00
volume
73
pages
1111 - 1116
publisher
Elsevier
external identifiers
  • wos:000277763800008
  • pmid:19995621
  • scopus:77950516408
ISSN
1874-3919
DOI
10.1016/j.jprot.2009.11.014
language
English
LU publication?
yes
id
03e64dae-af3f-429e-9c3f-e368dbcaddd7 (old id 1523788)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19995621?dopt=Abstract
date added to LUP
2010-01-14 13:05:35
date last changed
2018-05-29 09:57:42
@article{03e64dae-af3f-429e-9c3f-e368dbcaddd7,
  abstract     = {In this study, cell nuclei from aneuploid breast cancer samples were sorted with respect to DNA content into pure diploid and aneuploid fractions using flow cytometry. The nuclear proteins were then separated by one-dimensional gel electrophoresis (1D-PAGE) and differences in protein expression patterns, between diploid and aneuploid nuclei from the same tumours, were compared. Using a combination of peptide finger printing and peptide identification by MALDI-TOF mass spectrometry, we identified proteins and confirmed that the proteins were of nuclear origins. The results in this study add further information to the knowledge about the breast cancer disease complexity and heterogeneity at molecular level. For some of the tumours studied different nuclei protein patterns were obtained, in the diploid respective aneuploid nuclei populations, whilst other tumours did not show these differences.},
  author       = {Baldetorp, Bo and Johansson, Maria C and Ingvar, Christian and Lundgren, Lotta and Olsson, Håkan and Jansson, Bo and Welinder, Charlotte},
  issn         = {1874-3919},
  language     = {eng},
  pages        = {1111--1116},
  publisher    = {Elsevier},
  series       = {Journal of Proteomics2008-01-01+01:00},
  title        = {Analysis of protein expression in pure cell nuclei populations isolated from human breast cancer tissue by DNA flow cytometric sorting.},
  url          = {http://dx.doi.org/10.1016/j.jprot.2009.11.014},
  volume       = {73},
  year         = {2010},
}