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Heptemerones A-G, seven novel diterpenoids from Coprinus heptemerus: Producing organism, fermentation, isolation and biological activities

Kettering, M; Valdivia, Carola LU ; Sterner, Olov LU ; Anke, H and Thines, E (2005) In Journal of Antibiotics 58(6). p.390-396
Abstract
Seven novel diterpenoids, named heptemerones A-G, were isolated from the broth of submerged cultures of Coprinus heptemerus, a basidiomycete which previously had not been known to produce secondary metabolites. The compounds were purified by solid phase extraction and silica gel chromatography followed by preparative HPLC. Among the biological activities the inhibition of fungal germination was the most potent, and depended highly on the composition of the assay medium. In water, inhibition occurred at 5 similar to 10 fold lower concentrations as compared to complex media. Heptemerone G was the most active compound with MICs starting at 1 mu g/ml. Four of the antifungal compounds exhibited plant protective activity in a leaf segment assay... (More)
Seven novel diterpenoids, named heptemerones A-G, were isolated from the broth of submerged cultures of Coprinus heptemerus, a basidiomycete which previously had not been known to produce secondary metabolites. The compounds were purified by solid phase extraction and silica gel chromatography followed by preparative HPLC. Among the biological activities the inhibition of fungal germination was the most potent, and depended highly on the composition of the assay medium. In water, inhibition occurred at 5 similar to 10 fold lower concentrations as compared to complex media. Heptemerone G was the most active compound with MICs starting at 1 mu g/ml. Four of the antifungal compounds exhibited plant protective activity in a leaf segment assay using Magnaporthe grisea as the pathogen. Growth of yeasts and bacteria was hardly affected. Cytotoxic activities were moderate and only heptemerone D was phytotoxic. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Antibiotics
volume
58
issue
6
pages
390 - 396
publisher
Japan Antibiotics Research Association
external identifiers
  • wos:000230261300003
  • pmid:16156515
  • scopus:22444434591
ISSN
0021-8820
language
English
LU publication?
yes
id
6d3def7c-327a-41e4-9d90-125b1dd00239 (old id 152385)
alternative location
http://www.antibiotics.or.jp/journal/ja-top.htm
date added to LUP
2007-07-10 12:07:07
date last changed
2017-10-29 04:22:25
@article{6d3def7c-327a-41e4-9d90-125b1dd00239,
  abstract     = {Seven novel diterpenoids, named heptemerones A-G, were isolated from the broth of submerged cultures of Coprinus heptemerus, a basidiomycete which previously had not been known to produce secondary metabolites. The compounds were purified by solid phase extraction and silica gel chromatography followed by preparative HPLC. Among the biological activities the inhibition of fungal germination was the most potent, and depended highly on the composition of the assay medium. In water, inhibition occurred at 5 similar to 10 fold lower concentrations as compared to complex media. Heptemerone G was the most active compound with MICs starting at 1 mu g/ml. Four of the antifungal compounds exhibited plant protective activity in a leaf segment assay using Magnaporthe grisea as the pathogen. Growth of yeasts and bacteria was hardly affected. Cytotoxic activities were moderate and only heptemerone D was phytotoxic.},
  author       = {Kettering, M and Valdivia, Carola and Sterner, Olov and Anke, H and Thines, E},
  issn         = {0021-8820},
  language     = {eng},
  number       = {6},
  pages        = {390--396},
  publisher    = {Japan Antibiotics Research Association},
  series       = {Journal of Antibiotics},
  title        = {Heptemerones A-G, seven novel diterpenoids from Coprinus heptemerus: Producing organism, fermentation, isolation and biological activities},
  volume       = {58},
  year         = {2005},
}