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Whole gene family expression and drought stress regulation of aquaporins

Alexandersson, Erik LU ; Fraysse, Laure LU ; Sjövall Larsen, Sara LU ; Gustavsson, Sofia LU ; Agemark, Maria LU ; Karlsson, Maria LU ; Johanson, Urban LU and Kjellbom, Per LU (2005) In Plant Molecular Biology 59(3). p.469-484
Abstract
Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs)... (More)
Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs) have a high level of expression, while NOD26-like proteins (NIPs) are present at a much lower level. In addition, we show that PIP transcripts are generally down-regulated upon gradual drought stress in leaves, with the exception of AtPIP1;4 and AtPIP2;5, which are up-regulated. AtPIP2;6 and AtSIP1;1 are constitutively expressed and not significantly affected by the drought stress. The transcriptional down-regulation of PIP genes upon drought stress could also be observed on the protein level. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Molecular Biology
volume
59
issue
3
pages
469 - 484
publisher
Kluwer
external identifiers
  • wos:000232694400006
  • pmid:16235111
  • scopus:26844456576
ISSN
1573-5028
DOI
10.1007/s11103-005-0352-1
language
English
LU publication?
yes
id
57df30ae-4e6d-4ac1-aaf0-4d657e40f327 (old id 152447)
date added to LUP
2007-06-26 11:05:44
date last changed
2017-11-12 03:19:39
@article{57df30ae-4e6d-4ac1-aaf0-4d657e40f327,
  abstract     = {Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs) have a high level of expression, while NOD26-like proteins (NIPs) are present at a much lower level. In addition, we show that PIP transcripts are generally down-regulated upon gradual drought stress in leaves, with the exception of AtPIP1;4 and AtPIP2;5, which are up-regulated. AtPIP2;6 and AtSIP1;1 are constitutively expressed and not significantly affected by the drought stress. The transcriptional down-regulation of PIP genes upon drought stress could also be observed on the protein level.},
  author       = {Alexandersson, Erik and Fraysse, Laure and Sjövall Larsen, Sara and Gustavsson, Sofia and Agemark, Maria and Karlsson, Maria and Johanson, Urban and Kjellbom, Per},
  issn         = {1573-5028},
  language     = {eng},
  number       = {3},
  pages        = {469--484},
  publisher    = {Kluwer},
  series       = {Plant Molecular Biology},
  title        = {Whole gene family expression and drought stress regulation of aquaporins},
  url          = {http://dx.doi.org/10.1007/s11103-005-0352-1},
  volume       = {59},
  year         = {2005},
}