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Enhanced protein adsorption due to charge regulation

Lund, Mikael LU ; Åkesson, Torbjörn LU and Jönsson, Bo LU (2005) In Langmuir 21(18). p.8385-8388
Abstract
When a protein molecule approaches a charged surface, its protonation state can undergo dramatic changes due to the imposed electric potential. This has a large impact on adsorption strengths that may be enhanced by several kT. Using mesoscopic simulation techniques as well as analytical theories, we have investigated this regulation mechanism and demonstrate how it is influenced by salt concentration and solution pH. Using hisactophilin as a test case, we show how the binding to a lipid membrane is governed by small changes in pH and that this is intimately coupled to the charge regulation mechanism.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
21
issue
18
pages
8385 - 8388
publisher
The American Chemical Society
external identifiers
  • wos:000231550000044
  • scopus:24944550836
ISSN
0743-7463
DOI
10.1021/la050607z
language
English
LU publication?
yes
id
b9508064-e415-49da-bcdc-7e04a5be6620 (old id 152706)
date added to LUP
2007-07-17 11:59:39
date last changed
2017-11-05 03:33:34
@article{b9508064-e415-49da-bcdc-7e04a5be6620,
  abstract     = {When a protein molecule approaches a charged surface, its protonation state can undergo dramatic changes due to the imposed electric potential. This has a large impact on adsorption strengths that may be enhanced by several kT. Using mesoscopic simulation techniques as well as analytical theories, we have investigated this regulation mechanism and demonstrate how it is influenced by salt concentration and solution pH. Using hisactophilin as a test case, we show how the binding to a lipid membrane is governed by small changes in pH and that this is intimately coupled to the charge regulation mechanism.},
  author       = {Lund, Mikael and Åkesson, Torbjörn and Jönsson, Bo},
  issn         = {0743-7463},
  language     = {eng},
  number       = {18},
  pages        = {8385--8388},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Enhanced protein adsorption due to charge regulation},
  url          = {http://dx.doi.org/10.1021/la050607z},
  volume       = {21},
  year         = {2005},
}