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Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genes

Wang, Mei LU ; Ahrné, Siv LU ; Jeppsson, Bengt LU and Molin, Göran LU (2005) In FEMS Microbiology Ecology 54(2). p.219-231
Abstract
Bacterial diversity of the mucosal biopsies from human jejunum, distal ileum, ascending colon and rectum were compared by analysis of PCR-amplified 16S rDNA clone libraries. A total of 347 clones from the mucosal biopsies were partially sequenced and assigned to six phylogenctic phyla of the domain Bacteria: Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Verrucomicrobia, and Actinobacteria. The jejunum sample had least microbial diversity compared to the other samples and a trend towards highest diversity in ascending colon was observed. The clone libraries of distal ileum, ascending colon and rectum were not significantly different from each other (P > 0.0043), but they differed significantly from the jejunum library (P =... (More)
Bacterial diversity of the mucosal biopsies from human jejunum, distal ileum, ascending colon and rectum were compared by analysis of PCR-amplified 16S rDNA clone libraries. A total of 347 clones from the mucosal biopsies were partially sequenced and assigned to six phylogenctic phyla of the domain Bacteria: Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Verrucomicrobia, and Actinobacteria. The jejunum sample had least microbial diversity compared to the other samples and a trend towards highest diversity in ascending colon was observed. The clone libraries of distal ileum, ascending colon and rectum were not significantly different from each other (P > 0.0043), but they differed significantly from the jejunum library (P = 0.001). The population of sequences retrieved from jejunal biopsies was dominated by sequences closely related to Streptococcus (67%), while the population of sequences derived from distal ileum, ascending colon and rectum were dominated by sequences affiliated with Bacteroidetes (27-49%), and Clostridium clusters XlVa (20-34%) and IV (7-13%). The results indicate that the microbial community in jejunum is different from those in distal ileum, ascending colon and rectum, and that the major phylogenetic groups are similar from distal ileum to rectum. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Human mucosal biopsy, 16S rDNA cloning and sequencing, Bacterial diversity
in
FEMS Microbiology Ecology
volume
54
issue
2
pages
219 - 231
publisher
Oxford University Press
external identifiers
  • wos:000232428100006
  • pmid:16332321
  • scopus:24944540682
  • pmid:16332321
ISSN
1574-6941
DOI
10.1016/j.femsec.2005.03.012
language
English
LU publication?
yes
id
2decf292-e9aa-4dd8-a0a1-5786fc1eacee (old id 152840)
date added to LUP
2016-04-01 15:52:16
date last changed
2020-12-08 02:12:19
@article{2decf292-e9aa-4dd8-a0a1-5786fc1eacee,
  abstract     = {Bacterial diversity of the mucosal biopsies from human jejunum, distal ileum, ascending colon and rectum were compared by analysis of PCR-amplified 16S rDNA clone libraries. A total of 347 clones from the mucosal biopsies were partially sequenced and assigned to six phylogenctic phyla of the domain Bacteria: Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Verrucomicrobia, and Actinobacteria. The jejunum sample had least microbial diversity compared to the other samples and a trend towards highest diversity in ascending colon was observed. The clone libraries of distal ileum, ascending colon and rectum were not significantly different from each other (P > 0.0043), but they differed significantly from the jejunum library (P = 0.001). The population of sequences retrieved from jejunal biopsies was dominated by sequences closely related to Streptococcus (67%), while the population of sequences derived from distal ileum, ascending colon and rectum were dominated by sequences affiliated with Bacteroidetes (27-49%), and Clostridium clusters XlVa (20-34%) and IV (7-13%). The results indicate that the microbial community in jejunum is different from those in distal ileum, ascending colon and rectum, and that the major phylogenetic groups are similar from distal ileum to rectum. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.},
  author       = {Wang, Mei and Ahrné, Siv and Jeppsson, Bengt and Molin, Göran},
  issn         = {1574-6941},
  language     = {eng},
  number       = {2},
  pages        = {219--231},
  publisher    = {Oxford University Press},
  series       = {FEMS Microbiology Ecology},
  title        = {Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genes},
  url          = {http://dx.doi.org/10.1016/j.femsec.2005.03.012},
  doi          = {10.1016/j.femsec.2005.03.012},
  volume       = {54},
  year         = {2005},
}