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Three systems used for biocatalysis in organic solvents a comparative study

Larsson, Karin M. ; Janssen, Anja ; Adlercreutz, Patrick LU and Mattiasson, Bo LU (1990) In Biocatalysis and Biotransformation 4(2-3). p.163-175
Abstract

The activity and operational stability of horse liver alcohol dehydrogenase (HLADH) and αchymotrypsin were investigated in three systems commonly used for biocatalysis in organic solvents: 1. enzyme adsorbed on a solid support (celite) and added to the organic solvent (isooctane) 2. enzyme powder directly added to the organic solvent (isooctane). 3. enzyme dissolved in a microemulsion (AOT/isooctane). The activity and the operational stability in all systems were strongly dependent on the water content. The initial reaction rate was high in both the microemulsion and the celite system, but was much lower when adding the enzymes directly to the organic solvent. HLADH was observed to be more stable when added directly to the organic... (More)

The activity and operational stability of horse liver alcohol dehydrogenase (HLADH) and αchymotrypsin were investigated in three systems commonly used for biocatalysis in organic solvents: 1. enzyme adsorbed on a solid support (celite) and added to the organic solvent (isooctane) 2. enzyme powder directly added to the organic solvent (isooctane). 3. enzyme dissolved in a microemulsion (AOT/isooctane). The activity and the operational stability in all systems were strongly dependent on the water content. The initial reaction rate was high in both the microemulsion and the celite system, but was much lower when adding the enzymes directly to the organic solvent. HLADH was observed to be more stable when added directly to the organic solvent or dissolved in the microemulsion than when adsorbed on celite, whereas for αchymotrypsin stability was higher when adsorbed on celite or added directly to the organic solvent. For a hydrolytic reaction, a microemulsion was preferred due to the high water content. When adding the enzymes directly to the organic solvent both HLADH and chymotrypsin were adsorbed strongly to the glass walls of the reaction vessel. None of the systems were superior in all respects for the two enzymes studied.

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Contribution to journal
publication status
published
subject
keywords
Bioorganic synthesis, Directly added to organic solvent, Horse liver alcohol dehydrogenase, Microemulsion, Solid support, αchymotrypsin
in
Biocatalysis and Biotransformation
volume
4
issue
2-3
pages
13 pages
publisher
Taylor & Francis
external identifiers
  • scopus:26444455654
ISSN
1024-2422
DOI
10.3109/10242429008992088
language
English
LU publication?
yes
id
152b882d-e63b-41ce-8446-1e6dd757e887
date added to LUP
2019-06-22 18:48:44
date last changed
2020-01-13 02:05:18
@article{152b882d-e63b-41ce-8446-1e6dd757e887,
  abstract     = {<p>The activity and operational stability of horse liver alcohol dehydrogenase (HLADH) and αchymotrypsin were investigated in three systems commonly used for biocatalysis in organic solvents: 1. enzyme adsorbed on a solid support (celite) and added to the organic solvent (isooctane) 2. enzyme powder directly added to the organic solvent (isooctane). 3. enzyme dissolved in a microemulsion (AOT/isooctane). The activity and the operational stability in all systems were strongly dependent on the water content. The initial reaction rate was high in both the microemulsion and the celite system, but was much lower when adding the enzymes directly to the organic solvent. HLADH was observed to be more stable when added directly to the organic solvent or dissolved in the microemulsion than when adsorbed on celite, whereas for αchymotrypsin stability was higher when adsorbed on celite or added directly to the organic solvent. For a hydrolytic reaction, a microemulsion was preferred due to the high water content. When adding the enzymes directly to the organic solvent both HLADH and chymotrypsin were adsorbed strongly to the glass walls of the reaction vessel. None of the systems were superior in all respects for the two enzymes studied.</p>},
  author       = {Larsson, Karin M. and Janssen, Anja and Adlercreutz, Patrick and Mattiasson, Bo},
  issn         = {1024-2422},
  language     = {eng},
  month        = {01},
  number       = {2-3},
  pages        = {163--175},
  publisher    = {Taylor & Francis},
  series       = {Biocatalysis and Biotransformation},
  title        = {Three systems used for biocatalysis in organic solvents a comparative study},
  url          = {http://dx.doi.org/10.3109/10242429008992088},
  doi          = {10.3109/10242429008992088},
  volume       = {4},
  year         = {1990},
}