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Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells

Hebrard, Claire; Cros-Perrial, Emeline; Clausen, Anders Ranegaard LU ; Dumontet, Charles; Piskur, Jure LU and Jordheim, Lars Petter (2009) In Nucleosides, Nucleotides & Nucleic Acids 28(11-12). p.1068-1075
Abstract
Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bacteria, suicide gene, gene therapy, Deoxyribonucleoside kinases, gemcitabine, cancer, resistance
in
Nucleosides, Nucleotides & Nucleic Acids
volume
28
issue
11-12
pages
1068 - 1075
publisher
Taylor & Francis
external identifiers
  • wos:000273107100008
  • scopus:77949633741
ISSN
1525-7770
DOI
10.1080/15257770903368393
language
English
LU publication?
yes
id
0b124203-7d16-46ab-bf05-47f9a4a6d254 (old id 1531546)
date added to LUP
2010-01-28 11:48:12
date last changed
2017-01-01 04:48:49
@article{0b124203-7d16-46ab-bf05-47f9a4a6d254,
  abstract     = {Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.},
  author       = {Hebrard, Claire and Cros-Perrial, Emeline and Clausen, Anders Ranegaard and Dumontet, Charles and Piskur, Jure and Jordheim, Lars Petter},
  issn         = {1525-7770},
  keyword      = {bacteria,suicide gene,gene therapy,Deoxyribonucleoside kinases,gemcitabine,cancer,resistance},
  language     = {eng},
  number       = {11-12},
  pages        = {1068--1075},
  publisher    = {Taylor & Francis},
  series       = {Nucleosides, Nucleotides & Nucleic Acids},
  title        = {Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells},
  url          = {http://dx.doi.org/10.1080/15257770903368393},
  volume       = {28},
  year         = {2009},
}