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Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae

Forsgren, Eva; Olofsson, Tobias LU ; Vasquez, Alejandra LU and Fries, Ingemar (2010) In Apidologie 41(1). p.99-108
Abstract
We evaluated the antagonistic effects of newly identified lactic acid bacteria (LAB) in the genera Lactobacillus and Bifidobacterium, originating from the honey stomach, on the honey bee pathogen, Paenibacillus larvae. We used inhibition assays on agar plates and honey bee larval bioassays to investigate the effects of honey bee LAB on P. larvae growth in vitro and on AFB infection in vivo. The individual LAB phylotypes showed different inhibition properties against P. larvae growth on agar plates, whereas a combination of all eleven LAB phylotypes resulted in a total inhibition (no visible growth) of P. larvae. Adding the LAB mixture to the larval food significantly reduced the number of AFB infected larvae in exposure bioassays. The... (More)
We evaluated the antagonistic effects of newly identified lactic acid bacteria (LAB) in the genera Lactobacillus and Bifidobacterium, originating from the honey stomach, on the honey bee pathogen, Paenibacillus larvae. We used inhibition assays on agar plates and honey bee larval bioassays to investigate the effects of honey bee LAB on P. larvae growth in vitro and on AFB infection in vivo. The individual LAB phylotypes showed different inhibition properties against P. larvae growth on agar plates, whereas a combination of all eleven LAB phylotypes resulted in a total inhibition (no visible growth) of P. larvae. Adding the LAB mixture to the larval food significantly reduced the number of AFB infected larvae in exposure bioassays. The results demonstrate that honey bee specific LAB possess beneficial properties for honey bee health. Possible benefits to honey bee health by enhancing growth of LAB or by applying LAB to honey bee colonies should be further investigated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
inhibition, Bifidobacterium, Lactobacillus, Lactic Acid Bacteria, American foulbrood, Paenibacillus larvae
in
Apidologie
volume
41
issue
1
pages
99 - 108
publisher
Springer
external identifiers
  • wos:000273050600013
  • scopus:73449086690
ISSN
0044-8435
DOI
10.1051/apido/2009065
language
English
LU publication?
yes
id
7f5140e7-bb57-495a-9f87-098ba05fa2f9 (old id 1535532)
date added to LUP
2010-01-27 09:10:56
date last changed
2018-07-01 03:46:56
@article{7f5140e7-bb57-495a-9f87-098ba05fa2f9,
  abstract     = {We evaluated the antagonistic effects of newly identified lactic acid bacteria (LAB) in the genera Lactobacillus and Bifidobacterium, originating from the honey stomach, on the honey bee pathogen, Paenibacillus larvae. We used inhibition assays on agar plates and honey bee larval bioassays to investigate the effects of honey bee LAB on P. larvae growth in vitro and on AFB infection in vivo. The individual LAB phylotypes showed different inhibition properties against P. larvae growth on agar plates, whereas a combination of all eleven LAB phylotypes resulted in a total inhibition (no visible growth) of P. larvae. Adding the LAB mixture to the larval food significantly reduced the number of AFB infected larvae in exposure bioassays. The results demonstrate that honey bee specific LAB possess beneficial properties for honey bee health. Possible benefits to honey bee health by enhancing growth of LAB or by applying LAB to honey bee colonies should be further investigated.},
  author       = {Forsgren, Eva and Olofsson, Tobias and Vasquez, Alejandra and Fries, Ingemar},
  issn         = {0044-8435},
  keyword      = {inhibition,Bifidobacterium,Lactobacillus,Lactic Acid Bacteria,American foulbrood,Paenibacillus larvae},
  language     = {eng},
  number       = {1},
  pages        = {99--108},
  publisher    = {Springer},
  series       = {Apidologie},
  title        = {Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae},
  url          = {http://dx.doi.org/10.1051/apido/2009065},
  volume       = {41},
  year         = {2010},
}