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DNA and cationic surfactant complexes at hydrophilic surfaces. An ellipsometry and surface force study

Cárdenas, Marité LU ; Campos, José LU ; Nylander, Tommy LU and Lindman, Björn LU (2004) In Langmuir 20(20). p.8597-8603
Abstract
The adsorption and formation of DNA and cationic surfactant complexes at the silica-aqueous interface have been studied by ellipsometry. The interaction between the DNA-surfactant complexes at the mica-aqueous interface has been determined by the interferometric surface force apparatus. Adsorption was as expected not observed on negatively charged hydrophilic surfaces for DNA and when DNA-cationic surfactant complexes were negatively charged. However, adsorption was observed when there is an excess of cationic surfactant, just below the point of phase separation. The adsorption process requires hours to reach steady state. The adsorbed layer thickness is large at low surface coverage but becomes more compact and thinner at high coverage. A... (More)
The adsorption and formation of DNA and cationic surfactant complexes at the silica-aqueous interface have been studied by ellipsometry. The interaction between the DNA-surfactant complexes at the mica-aqueous interface has been determined by the interferometric surface force apparatus. Adsorption was as expected not observed on negatively charged hydrophilic surfaces for DNA and when DNA-cationic surfactant complexes were negatively charged. However, adsorption was observed when there is an excess of cationic surfactant, just below the point of phase separation. The adsorption process requires hours to reach steady state. The adsorbed layer thickness is large at low surface coverage but becomes more compact and thinner at high coverage. A long-range repulsive force was observed between adsorbed layers of DNA-cationic surfactant complexes, which was suggested to be of both electrostatic and steric origin. The forces were found to be dependent on the equilibration time and the experimental pathway. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
20
issue
20
pages
8597 - 8603
publisher
The American Chemical Society
external identifiers
  • wos:000224039000029
  • pmid:15379480
  • scopus:5444248003
ISSN
0743-7463
DOI
10.1021/la0363581
language
English
LU publication?
yes
id
2b90935e-1254-4e7c-ac0a-a261c3e7d596 (old id 153866)
date added to LUP
2007-07-11 09:21:32
date last changed
2017-08-27 04:22:27
@article{2b90935e-1254-4e7c-ac0a-a261c3e7d596,
  abstract     = {The adsorption and formation of DNA and cationic surfactant complexes at the silica-aqueous interface have been studied by ellipsometry. The interaction between the DNA-surfactant complexes at the mica-aqueous interface has been determined by the interferometric surface force apparatus. Adsorption was as expected not observed on negatively charged hydrophilic surfaces for DNA and when DNA-cationic surfactant complexes were negatively charged. However, adsorption was observed when there is an excess of cationic surfactant, just below the point of phase separation. The adsorption process requires hours to reach steady state. The adsorbed layer thickness is large at low surface coverage but becomes more compact and thinner at high coverage. A long-range repulsive force was observed between adsorbed layers of DNA-cationic surfactant complexes, which was suggested to be of both electrostatic and steric origin. The forces were found to be dependent on the equilibration time and the experimental pathway.},
  author       = {Cárdenas, Marité and Campos, José and Nylander, Tommy and Lindman, Björn},
  issn         = {0743-7463},
  language     = {eng},
  number       = {20},
  pages        = {8597--8603},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {DNA and cationic surfactant complexes at hydrophilic surfaces. An ellipsometry and surface force study},
  url          = {http://dx.doi.org/10.1021/la0363581},
  volume       = {20},
  year         = {2004},
}