Advanced

Interactions of cyclic AMP and its dibutyryl analogue with a lipid layer in the aqueous mixtures of monoolein preparation and dioleoyl phosphatidylcholine as probed by X-ray diffraction and Raman spectroscopy

Talaikyte, Z; Barauskas, Justas LU ; Niaura, G; Svedaite, I; Butkus, E; Razumas, V and Nylander, Tommy LU (2004) In Journal of Biological Physics 30(1). p.83-96
Abstract
Interactions of adenosine 3':5'-cyclic monophosphate (cAMP) and N-6,2'-O-dibutyryl-adenosine 3':5'-cyclic monophosphate (dbcAMP) with a lipid layer composed of monoolein-based preparation and dioleoyl phosphatidylcholine ( DOPC) were investigated by small-angle X-ray diffraction (SAXD) and Raman spectroscopy. The reversed hexagonal (H-II) MO/DOPC/H2O phase of 65:15:20 wt.% composition was selected as a reference system. SAXD revealed that entrapment (at the expense of water) of 3 wt.% cAMP into the reference system did not change the polymorphic form and structural parameters of the phase. The same content of dbcAMP induced the transition from the HII phase to the reversed bicontinuous cubic phase of space group Ia3d. This transition is... (More)
Interactions of adenosine 3':5'-cyclic monophosphate (cAMP) and N-6,2'-O-dibutyryl-adenosine 3':5'-cyclic monophosphate (dbcAMP) with a lipid layer composed of monoolein-based preparation and dioleoyl phosphatidylcholine ( DOPC) were investigated by small-angle X-ray diffraction (SAXD) and Raman spectroscopy. The reversed hexagonal (H-II) MO/DOPC/H2O phase of 65:15:20 wt.% composition was selected as a reference system. SAXD revealed that entrapment (at the expense of water) of 3 wt.% cAMP into the reference system did not change the polymorphic form and structural parameters of the phase. The same content of dbcAMP induced the transition from the HII phase to the reversed bicontinuous cubic phase of space group Ia3d. This transition is explained by the increase of lipid head-group area due to the penetration of the acylated adenine group of dbcAMP into the polar/apolar region of lipid layer. The conclusion is supported by Raman spectroscopy, showing the disruption/weakening of hydrogen bonding in the MO/DOPC-based matrix at the N1- and N3-sites of the dbcAMP adenine ring. As distinct from dbcAMP, cAMP remains mostly in the water channels of the HII phase, although the phosphate residue of nucleotide interacts with the quaternary ammonium group of DOPC. Both nucleotides increase the population of gauche isomers in the DOPC choline group. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
diffraction, X-ray, Raman spectroscopy, monoolein, liquid-crystalline phases, dibutyryl cyclic AMP, dioleoyl phosphatidylcholine, cyclic AMP
in
Journal of Biological Physics
volume
30
issue
1
pages
83 - 96
publisher
Kluwer
external identifiers
  • wos:000189013900003
  • scopus:1542410498
ISSN
0092-0606
DOI
10.1023/B:JOBP.0000016569.40786.2e
language
English
LU publication?
yes
id
93bac66c-701c-447a-a77d-d3af958cc6f7 (old id 154045)
date added to LUP
2007-07-12 12:18:15
date last changed
2017-07-23 04:42:49
@article{93bac66c-701c-447a-a77d-d3af958cc6f7,
  abstract     = {Interactions of adenosine 3':5'-cyclic monophosphate (cAMP) and N-6,2'-O-dibutyryl-adenosine 3':5'-cyclic monophosphate (dbcAMP) with a lipid layer composed of monoolein-based preparation and dioleoyl phosphatidylcholine ( DOPC) were investigated by small-angle X-ray diffraction (SAXD) and Raman spectroscopy. The reversed hexagonal (H-II) MO/DOPC/H2O phase of 65:15:20 wt.% composition was selected as a reference system. SAXD revealed that entrapment (at the expense of water) of 3 wt.% cAMP into the reference system did not change the polymorphic form and structural parameters of the phase. The same content of dbcAMP induced the transition from the HII phase to the reversed bicontinuous cubic phase of space group Ia3d. This transition is explained by the increase of lipid head-group area due to the penetration of the acylated adenine group of dbcAMP into the polar/apolar region of lipid layer. The conclusion is supported by Raman spectroscopy, showing the disruption/weakening of hydrogen bonding in the MO/DOPC-based matrix at the N1- and N3-sites of the dbcAMP adenine ring. As distinct from dbcAMP, cAMP remains mostly in the water channels of the HII phase, although the phosphate residue of nucleotide interacts with the quaternary ammonium group of DOPC. Both nucleotides increase the population of gauche isomers in the DOPC choline group.},
  author       = {Talaikyte, Z and Barauskas, Justas and Niaura, G and Svedaite, I and Butkus, E and Razumas, V and Nylander, Tommy},
  issn         = {0092-0606},
  keyword      = {diffraction,X-ray,Raman spectroscopy,monoolein,liquid-crystalline phases,dibutyryl cyclic AMP,dioleoyl phosphatidylcholine,cyclic AMP},
  language     = {eng},
  number       = {1},
  pages        = {83--96},
  publisher    = {Kluwer},
  series       = {Journal of Biological Physics},
  title        = {Interactions of cyclic AMP and its dibutyryl analogue with a lipid layer in the aqueous mixtures of monoolein preparation and dioleoyl phosphatidylcholine as probed by X-ray diffraction and Raman spectroscopy},
  url          = {http://dx.doi.org/10.1023/B:JOBP.0000016569.40786.2e},
  volume       = {30},
  year         = {2004},
}