Advanced

Binding of complement regulators to invasive nontypeable Haemophilus influenzae is not increased compared to nasopharyngeal isolates, but serum resistance is linked to disease severity.

Hallström, Teresia LU ; Resman, Fredrik LU ; Ristovski, Mikael and Riesbeck, Kristian LU (2010) In Journal of Clinical Microbiology 48(3). p.921-927
Abstract
The aim of the present study was to analyse the importance for non-typeable Haemophilus influenzae (NTHi) isolated from patients with sepsis (invasive isolates) compared to nasopharyngeal isolates from patients with upper respiratory tract infection to resist the complement-mediated attack in human serum and to correlate this to disease severity. We in detail studied and characterized cases of invasive NTHi disease. All patients with invasive NTHi isolates were adults and 35 % had a clinical presentation of severe sepsis according to the ACCP/SCCM classification of sepsis grading. Moreover, 41 % of the cases had evidence of immune deficiency. The different isolates were analyzed for survival in human serum, for binding of [(125)I]-labeled... (More)
The aim of the present study was to analyse the importance for non-typeable Haemophilus influenzae (NTHi) isolated from patients with sepsis (invasive isolates) compared to nasopharyngeal isolates from patients with upper respiratory tract infection to resist the complement-mediated attack in human serum and to correlate this to disease severity. We in detail studied and characterized cases of invasive NTHi disease. All patients with invasive NTHi isolates were adults and 35 % had a clinical presentation of severe sepsis according to the ACCP/SCCM classification of sepsis grading. Moreover, 41 % of the cases had evidence of immune deficiency. The different isolates were analyzed for survival in human serum, for binding of [(125)I]-labeled purified human complement inhibitors C4b-binding protein (C4BP), Factor H and vitronectin in addition to binding of regulators directly from serum. No significant differences were found when blood and nasopharyngeal isolates were compared, suggesting that interactions with the complement system are equally important for NTHi strains irrespectively of isolation site. Interestingly, a correlation between serum resistance and invasive disease severity was found. The ability to resist the attack of the complement system seems to be important for NTHi strains infecting the respiratory tract as well as the blood stream. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
48
issue
3
pages
921 - 927
publisher
American Society for Microbiology
external identifiers
  • wos:000274996200038
  • pmid:20089757
  • scopus:77749340477
ISSN
1098-660X
DOI
10.1128/JCM.01654-09
language
English
LU publication?
yes
id
33c89ede-7f59-420a-9ee8-240746e2b4a1 (old id 1540740)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20089757?dopt=Abstract
date added to LUP
2010-02-04 15:58:05
date last changed
2018-05-29 10:55:15
@article{33c89ede-7f59-420a-9ee8-240746e2b4a1,
  abstract     = {The aim of the present study was to analyse the importance for non-typeable Haemophilus influenzae (NTHi) isolated from patients with sepsis (invasive isolates) compared to nasopharyngeal isolates from patients with upper respiratory tract infection to resist the complement-mediated attack in human serum and to correlate this to disease severity. We in detail studied and characterized cases of invasive NTHi disease. All patients with invasive NTHi isolates were adults and 35 % had a clinical presentation of severe sepsis according to the ACCP/SCCM classification of sepsis grading. Moreover, 41 % of the cases had evidence of immune deficiency. The different isolates were analyzed for survival in human serum, for binding of [(125)I]-labeled purified human complement inhibitors C4b-binding protein (C4BP), Factor H and vitronectin in addition to binding of regulators directly from serum. No significant differences were found when blood and nasopharyngeal isolates were compared, suggesting that interactions with the complement system are equally important for NTHi strains irrespectively of isolation site. Interestingly, a correlation between serum resistance and invasive disease severity was found. The ability to resist the attack of the complement system seems to be important for NTHi strains infecting the respiratory tract as well as the blood stream.},
  author       = {Hallström, Teresia and Resman, Fredrik and Ristovski, Mikael and Riesbeck, Kristian},
  issn         = {1098-660X},
  language     = {eng},
  number       = {3},
  pages        = {921--927},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {Binding of complement regulators to invasive nontypeable Haemophilus influenzae is not increased compared to nasopharyngeal isolates, but serum resistance is linked to disease severity.},
  url          = {http://dx.doi.org/10.1128/JCM.01654-09},
  volume       = {48},
  year         = {2010},
}