Peripheral nerve reconstruction. Studies on cellular and acellular iso-, allo- and xenografts
(2010) In Lund University Faculty of Medicine Doctoral Dissertation Series 2010:12.- Abstract
- The best results after a nerve injury with a defect are achieved by a nerve reconstruction procedure
using autologous nerve grafts. However, limitations include insufficient amount of suitable donor
material and donor site morbidity. The aim of the present thesis was to study alternative nerve grafts,
i.e. allo- and xenografts, in rats and mice. Cellular grafts will evoke a rejection in the host aiming at
destroying the graft. Thus, different strategies to suppress or avoid rejection were studied.
Treatment with the immunosuppressant FK506 increased axonal outgrowth after a nerve crush lesion,
but did not improve axonal outgrowth in autologous nerve grafts in rats.
... (More) - The best results after a nerve injury with a defect are achieved by a nerve reconstruction procedure
using autologous nerve grafts. However, limitations include insufficient amount of suitable donor
material and donor site morbidity. The aim of the present thesis was to study alternative nerve grafts,
i.e. allo- and xenografts, in rats and mice. Cellular grafts will evoke a rejection in the host aiming at
destroying the graft. Thus, different strategies to suppress or avoid rejection were studied.
Treatment with the immunosuppressant FK506 increased axonal outgrowth after a nerve crush lesion,
but did not improve axonal outgrowth in autologous nerve grafts in rats.
The new strategy of costimulation blockade that inhibits T-cell activation prohibited rejection of nerve
allografts in mice in the short term. Rejection of grafts in placebo-treated mice impeded axonal out-
growth. In the long-term, costimulation blockade inhibited rejection and resulted in an improved
myelination of regenerated axons. A higher target muscle weight indicated less denervation atrophy
and thus improved nerve regeneration after costimulation blockade.
To avoid immunosuppression altogether acellular nerve allo- and xenografts were studied. The best
axonal outgrowth was observed in allografts. The axonal outgrowth in xenografts was influenced by
the combination of host/donor species.
In conclusion, costimulation blockade inhibits rejection of nerve allografts and allows axonal outgrowth.
However, for reconstruction of an isolated severe nerve injury with a defect, the use of acellular nerve
allografts is a promising strategy. It addresses many of the limitations of autologous nerve grafts without
necessitating immunosuppression. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1544285
- author
- Kvist, Martin LU
- supervisor
-
- Lars Dahlin LU
- Martin Kanje LU
- opponent
-
- Professor Terenghi, Giorgio, Manchester, Storbritannien
- organization
- publishing date
- 2010
- type
- Thesis
- publication status
- published
- subject
- keywords
- acellular nerve graft, costimulation blockade, FK506, immunosuppression, nerve graft, nerve defect, nerve regeneration, nerve injury, nerve extraction, allograft, xenograft
- in
- Lund University Faculty of Medicine Doctoral Dissertation Series
- volume
- 2010:12
- pages
- 116 pages
- publisher
- Lund University, Dept of Hand Surgery
- defense location
- Lilla aulan, Medicinskt Forskningscentrum, Ingång 59, Skånes Universitetssjukhus, Malmö,
- defense date
- 2010-03-05 13:00:00
- ISSN
- 1652-8220
- ISBN
- 978-91-86443-26-9
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hand Surgery Research Group (013241910), Reconstructive Surgery (013240300)
- id
- 837dc867-a04a-43cf-9a7c-46c91377579e (old id 1544285)
- date added to LUP
- 2016-04-01 13:26:30
- date last changed
- 2019-05-21 23:07:48
@phdthesis{837dc867-a04a-43cf-9a7c-46c91377579e,
abstract = {{The best results after a nerve injury with a defect are achieved by a nerve reconstruction procedure <br/><br>
using autologous nerve grafts. However, limitations include insufficient amount of suitable donor <br/><br>
material and donor site morbidity. The aim of the present thesis was to study alternative nerve grafts, <br/><br>
i.e. allo- and xenografts, in rats and mice. Cellular grafts will evoke a rejection in the host aiming at <br/><br>
destroying the graft. Thus, different strategies to suppress or avoid rejection were studied.<br/><br>
Treatment with the immunosuppressant FK506 increased axonal outgrowth after a nerve crush lesion, <br/><br>
but did not improve axonal outgrowth in autologous nerve grafts in rats.<br/><br>
The new strategy of costimulation blockade that inhibits T-cell activation prohibited rejection of nerve <br/><br>
allografts in mice in the short term. Rejection of grafts in placebo-treated mice impeded axonal out-<br/><br>
growth. In the long-term, costimulation blockade inhibited rejection and resulted in an improved <br/><br>
myelination of regenerated axons. A higher target muscle weight indicated less denervation atrophy <br/><br>
and thus improved nerve regeneration after costimulation blockade.<br/><br>
To avoid immunosuppression altogether acellular nerve allo- and xenografts were studied. The best <br/><br>
axonal outgrowth was observed in allografts. The axonal outgrowth in xenografts was influenced by <br/><br>
the combination of host/donor species.<br/><br>
<br/><br>
In conclusion, costimulation blockade inhibits rejection of nerve allografts and allows axonal outgrowth. <br/><br>
However, for reconstruction of an isolated severe nerve injury with a defect, the use of acellular nerve <br/><br>
allografts is a promising strategy. It addresses many of the limitations of autologous nerve grafts without <br/><br>
necessitating immunosuppression.}},
author = {{Kvist, Martin}},
isbn = {{978-91-86443-26-9}},
issn = {{1652-8220}},
keywords = {{acellular nerve graft; costimulation blockade; FK506; immunosuppression; nerve graft; nerve defect; nerve regeneration; nerve injury; nerve extraction; allograft; xenograft}},
language = {{eng}},
publisher = {{Lund University, Dept of Hand Surgery}},
school = {{Lund University}},
series = {{Lund University Faculty of Medicine Doctoral Dissertation Series}},
title = {{Peripheral nerve reconstruction. Studies on cellular and acellular iso-, allo- and xenografts}},
volume = {{2010:12}},
year = {{2010}},
}