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Bioelectrochemical studies of azurin and laccase confined in three-dimensional chips based on gold-modified nano-/microstructured silicon

Ressine, Anton; Vaz-Dominguez, Cristina; Fernandez, Victor M.; De Lacey, Antonio L.; Laurell, Thomas LU ; Ruzgas, Tautgirdas and Shleev, Sergey (2010) In Biosensors & Bioelectronics 25(5). p.1001-1007
Abstract
Double-sided three-dimensional porous silicon chips, 6 mm x 6 mm, covered with a 40 nm gold (nano)layer, were fabricated from a porous silicon wafer. Scanning electron microscopy along with electrochemical characterisation showed sample conductivity, mechanical stability, and high surface area of the thus fabricated devices, viz. 10 times higher electrochemically active surface area compared to the geometric area. The three-dimensional gold coated silicon chips were further modified with thiol layers, followed by immobilisation of a simple copper-containing redox protein, azurin, or a complex multicopper redox enzyme, laccase. The bioelectrochemical studies showed very high surface concentrations of azurin and laccase, i.e. close to the... (More)
Double-sided three-dimensional porous silicon chips, 6 mm x 6 mm, covered with a 40 nm gold (nano)layer, were fabricated from a porous silicon wafer. Scanning electron microscopy along with electrochemical characterisation showed sample conductivity, mechanical stability, and high surface area of the thus fabricated devices, viz. 10 times higher electrochemically active surface area compared to the geometric area. The three-dimensional gold coated silicon chips were further modified with thiol layers, followed by immobilisation of a simple copper-containing redox protein, azurin, or a complex multicopper redox enzyme, laccase. The bioelectrochemical studies showed very high surface concentrations of azurin and laccase, i.e. close to the theoretical monolayer coverage. However, direct electron transfer reactions between the biomolecules and gold surfaces were observed only for a small percentage of the immobilised redox protein and enzyme, respectively. Thus, highly efficient oxygen-bioelectroreduction on laccase-modified 3D thiol-gold-porous silicon chips (as compared to planar laccase-modified gold electrodes, 42 mu A/cm(2) vs. 7 mu A/cm(2), respectively) was obtained only in the presence of an efficient soluble redox mediator. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biosensor, Porous silicon, Three-dimensional biochip, Biocathode, Azurin, Laccase
in
Biosensors & Bioelectronics
volume
25
issue
5
pages
1001 - 1007
publisher
Elsevier
external identifiers
  • wos:000273945800008
  • scopus:71849111847
ISSN
1873-4235
DOI
10.1016/j.bios.2009.09.014
language
English
LU publication?
yes
id
a0d1a1e9-c306-4974-ade6-e2c57e0ad6a7 (old id 1547039)
date added to LUP
2010-02-25 10:02:00
date last changed
2018-05-29 10:29:57
@article{a0d1a1e9-c306-4974-ade6-e2c57e0ad6a7,
  abstract     = {Double-sided three-dimensional porous silicon chips, 6 mm x 6 mm, covered with a 40 nm gold (nano)layer, were fabricated from a porous silicon wafer. Scanning electron microscopy along with electrochemical characterisation showed sample conductivity, mechanical stability, and high surface area of the thus fabricated devices, viz. 10 times higher electrochemically active surface area compared to the geometric area. The three-dimensional gold coated silicon chips were further modified with thiol layers, followed by immobilisation of a simple copper-containing redox protein, azurin, or a complex multicopper redox enzyme, laccase. The bioelectrochemical studies showed very high surface concentrations of azurin and laccase, i.e. close to the theoretical monolayer coverage. However, direct electron transfer reactions between the biomolecules and gold surfaces were observed only for a small percentage of the immobilised redox protein and enzyme, respectively. Thus, highly efficient oxygen-bioelectroreduction on laccase-modified 3D thiol-gold-porous silicon chips (as compared to planar laccase-modified gold electrodes, 42 mu A/cm(2) vs. 7 mu A/cm(2), respectively) was obtained only in the presence of an efficient soluble redox mediator. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Ressine, Anton and Vaz-Dominguez, Cristina and Fernandez, Victor M. and De Lacey, Antonio L. and Laurell, Thomas and Ruzgas, Tautgirdas and Shleev, Sergey},
  issn         = {1873-4235},
  keyword      = {Biosensor,Porous silicon,Three-dimensional biochip,Biocathode,Azurin,Laccase},
  language     = {eng},
  number       = {5},
  pages        = {1001--1007},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Bioelectrochemical studies of azurin and laccase confined in three-dimensional chips based on gold-modified nano-/microstructured silicon},
  url          = {http://dx.doi.org/10.1016/j.bios.2009.09.014},
  volume       = {25},
  year         = {2010},
}