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Efficient transduction of neurons using Ross River glycoprotein-pseudotyped lentiviral vectors.

Jakobsson, Johan LU orcid ; Tolstrup Nielsen, Troels LU ; Staflin, Karin LU ; Georgievska, Biljana LU and Lundberg, Cecilia LU orcid (2006) In Gene Therapy 13(12). p.966-973
Abstract
Lentiviral vectors are promising tools for CNS gene transfer since they efficiently transduce the cells of the nervous system in vivo. In this study, we have investigated the transduction efficiency of lentiviral vectors pseudotyped with Ross River virus glycoprotein (RRV-G) (RRV-G-pseudotyped lentiviral vectors (RRV-LV)). The RRV is an alphavirus with an extremely broad host range, including the cells of the central nervous system. Previous studies have shown that lentiviral vectors can be efficiently pseudotyped with this envelope protein and have demonstrated promising features of such vectors, including the possibility to establish stable producer cell lines. After injection of RRV-LV expressing green fluorescent protein into different... (More)
Lentiviral vectors are promising tools for CNS gene transfer since they efficiently transduce the cells of the nervous system in vivo. In this study, we have investigated the transduction efficiency of lentiviral vectors pseudotyped with Ross River virus glycoprotein (RRV-G) (RRV-G-pseudotyped lentiviral vectors (RRV-LV)). The RRV is an alphavirus with an extremely broad host range, including the cells of the central nervous system. Previous studies have shown that lentiviral vectors can be efficiently pseudotyped with this envelope protein and have demonstrated promising features of such vectors, including the possibility to establish stable producer cell lines. After injection of RRV-LV expressing green fluorescent protein into different structures in the rat brain we found efficient transduction of both neurons and glial cells. By using two cell-type-specific promoters, neuron-specific enolase and human glial fibrillary acidic protein, we demonstrated cell-specific transgene expression in the desired cell type. Ross River virus glycoprotein-pseudotyped lentiviral vectors also transduced human neural progenitor cells in vitro, showing that receptors for the RRV-G are present on human neural cells. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
green fluorescent protein, alphavirus, astrocyte, gene transfer
in
Gene Therapy
volume
13
issue
12
pages
966 - 973
publisher
Nature Publishing Group
external identifiers
  • pmid:16511527
  • wos:000238041000004
  • scopus:33744820829
  • pmid:16511527
ISSN
0969-7128
DOI
10.1038/sj.gt.3302701
language
English
LU publication?
yes
id
cd164568-bebd-4f09-9556-3fb825f57e46 (old id 154854)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16511527&dopt=Abstract
date added to LUP
2016-04-01 11:46:19
date last changed
2021-08-11 01:37:10
@article{cd164568-bebd-4f09-9556-3fb825f57e46,
  abstract     = {Lentiviral vectors are promising tools for CNS gene transfer since they efficiently transduce the cells of the nervous system in vivo. In this study, we have investigated the transduction efficiency of lentiviral vectors pseudotyped with Ross River virus glycoprotein (RRV-G) (RRV-G-pseudotyped lentiviral vectors (RRV-LV)). The RRV is an alphavirus with an extremely broad host range, including the cells of the central nervous system. Previous studies have shown that lentiviral vectors can be efficiently pseudotyped with this envelope protein and have demonstrated promising features of such vectors, including the possibility to establish stable producer cell lines. After injection of RRV-LV expressing green fluorescent protein into different structures in the rat brain we found efficient transduction of both neurons and glial cells. By using two cell-type-specific promoters, neuron-specific enolase and human glial fibrillary acidic protein, we demonstrated cell-specific transgene expression in the desired cell type. Ross River virus glycoprotein-pseudotyped lentiviral vectors also transduced human neural progenitor cells in vitro, showing that receptors for the RRV-G are present on human neural cells.},
  author       = {Jakobsson, Johan and Tolstrup Nielsen, Troels and Staflin, Karin and Georgievska, Biljana and Lundberg, Cecilia},
  issn         = {0969-7128},
  language     = {eng},
  number       = {12},
  pages        = {966--973},
  publisher    = {Nature Publishing Group},
  series       = {Gene Therapy},
  title        = {Efficient transduction of neurons using Ross River glycoprotein-pseudotyped lentiviral vectors.},
  url          = {http://dx.doi.org/10.1038/sj.gt.3302701},
  doi          = {10.1038/sj.gt.3302701},
  volume       = {13},
  year         = {2006},
}