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Pore structure in supermacroporous polyacrylamide based cryogels

Plieva, Fatima LU ; Sjöö, Malin LU ; Aguilar, Maria-Rosa; Gomez, David; Mikhalovsky, Sergey and Galaev, Igor LU (2005) In Soft Matter 1(4). p.303-309
Abstract
Pore size and thickness of pore walls in macroporous polyacrylamide gels, so-called cryogels (pAAm-cryogels), were controlled by varying the content of monomers in the initial reaction mixture and the cross-linker used. The thickness of pore walls in pAAm-cryogels increased with increasing concentration of monomers in the initial reaction mixture. Pore volume in the supermacroporous pAAm-cryogels was in the range of 70-93% and decreased with increasing concentration of monomers in the reaction feed. The porous structure of the pAAm-cryogels was visualized using environmental scanning electron microscopy (ESEM) that allowed monitoring of the dehydration process in pAAm-cryogels. The accessibility of ligands covalently coupled to the polymer... (More)
Pore size and thickness of pore walls in macroporous polyacrylamide gels, so-called cryogels (pAAm-cryogels), were controlled by varying the content of monomers in the initial reaction mixture and the cross-linker used. The thickness of pore walls in pAAm-cryogels increased with increasing concentration of monomers in the initial reaction mixture. Pore volume in the supermacroporous pAAm-cryogels was in the range of 70-93% and decreased with increasing concentration of monomers in the reaction feed. The porous structure of the pAAm-cryogels was visualized using environmental scanning electron microscopy (ESEM) that allowed monitoring of the dehydration process in pAAm-cryogels. The accessibility of ligands covalently coupled to the polymer backbone for low molecular weight target, Cu(II) ions, and high molecular weight target, the protein lysozyme, was assessed for pAAm-cryogels produced from feeds with different monomer concentration. The amount of bound Cu(II) ions increased linearly with increasing monomer concentration in the reaction feed, suggesting that all ligands are equally accessible for small targets. On the contrary, lysozyme binding demonstrated a clear maximum at monomer concentration about 18% suggesting that only ligrands present at the surface of pore walls are accessible for high molecular weight targets. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
1
issue
4
pages
303 - 309
publisher
Royal Society of Chemistry
external identifiers
  • wos:000232720200008
  • scopus:26944436252
ISSN
1744-6848
DOI
10.1039/b510010k
language
English
LU publication?
yes
id
b8df470d-216f-4101-8fc9-117bf45382e6 (old id 155026)
date added to LUP
2007-07-02 16:38:06
date last changed
2017-10-01 04:46:53
@article{b8df470d-216f-4101-8fc9-117bf45382e6,
  abstract     = {Pore size and thickness of pore walls in macroporous polyacrylamide gels, so-called cryogels (pAAm-cryogels), were controlled by varying the content of monomers in the initial reaction mixture and the cross-linker used. The thickness of pore walls in pAAm-cryogels increased with increasing concentration of monomers in the initial reaction mixture. Pore volume in the supermacroporous pAAm-cryogels was in the range of 70-93% and decreased with increasing concentration of monomers in the reaction feed. The porous structure of the pAAm-cryogels was visualized using environmental scanning electron microscopy (ESEM) that allowed monitoring of the dehydration process in pAAm-cryogels. The accessibility of ligands covalently coupled to the polymer backbone for low molecular weight target, Cu(II) ions, and high molecular weight target, the protein lysozyme, was assessed for pAAm-cryogels produced from feeds with different monomer concentration. The amount of bound Cu(II) ions increased linearly with increasing monomer concentration in the reaction feed, suggesting that all ligands are equally accessible for small targets. On the contrary, lysozyme binding demonstrated a clear maximum at monomer concentration about 18% suggesting that only ligrands present at the surface of pore walls are accessible for high molecular weight targets.},
  author       = {Plieva, Fatima and Sjöö, Malin and Aguilar, Maria-Rosa and Gomez, David and Mikhalovsky, Sergey and Galaev, Igor},
  issn         = {1744-6848},
  language     = {eng},
  number       = {4},
  pages        = {303--309},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {Pore structure in supermacroporous polyacrylamide based cryogels},
  url          = {http://dx.doi.org/10.1039/b510010k},
  volume       = {1},
  year         = {2005},
}