Advanced

A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor

de Maré, Lena LU ; Velut, Stéphane LU ; Ledung, E; Cimander, C; Norrman, B; Nordberg Karlsson, Eva LU ; Holst, Olle LU and Hagander, Per LU (2005) In Biotechnology Letters 27(14). p.983-990
Abstract
A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Letters
volume
27
issue
14
pages
983 - 990
publisher
Springer
external identifiers
  • pmid:16132841
  • wos:000231471000004
  • scopus:24044490195
ISSN
1573-6776
DOI
10.1007/s10529-005-7844-6
language
English
LU publication?
yes
id
670db968-2adb-4dd6-9e8e-e8b0d0685c54 (old id 155069)
date added to LUP
2007-07-03 09:42:01
date last changed
2017-10-01 04:42:31
@article{670db968-2adb-4dd6-9e8e-e8b0d0685c54,
  abstract     = {A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O-2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.},
  author       = {de Maré, Lena and Velut, Stéphane and Ledung, E and Cimander, C and Norrman, B and Nordberg Karlsson, Eva and Holst, Olle and Hagander, Per},
  issn         = {1573-6776},
  language     = {eng},
  number       = {14},
  pages        = {983--990},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {A cultivation technique for E-coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor},
  url          = {http://dx.doi.org/10.1007/s10529-005-7844-6},
  volume       = {27},
  year         = {2005},
}