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Essential tactics of tissue preparation and matrix nano-spotting for successful compound imaging mass spectrometry

Végvári, Ákos LU ; Fehniger, Thomas E.; Gustavsson, Lena; Nilsson, Anna; Andrén, Per E.; Kenne, Kerstin; Nilsson, Johan LU ; Laurell, Thomas LU and Marko-Varga, György LU (2010) In Journal of Proteomics2008-01-01+01:00 73(6). p.1270-1278
Abstract
The ultimate goal of MALDI-Imaging Mass Spectrometry (MALDI-IMS) is to achieve spatial localization of analytes in tissue sections down to individual tissue compartments or even at the level of a few cells. With compound tissue imaging, it is possible to track the transportation of an unlabelled, inhaled reference compound within lung tissue, through the application of MALDI-IMS. The procedure for isolation and preparation of lung tissues is found to be crucial in order to preserve the anatomy and structure of the pulmonary compartments.

To avoid delocalization of analytes within lung tissue compartments we have applied an in-house designed nano-spotter, based on a microdispenser mounted on XY-table, of which movement and... (More)
The ultimate goal of MALDI-Imaging Mass Spectrometry (MALDI-IMS) is to achieve spatial localization of analytes in tissue sections down to individual tissue compartments or even at the level of a few cells. With compound tissue imaging, it is possible to track the transportation of an unlabelled, inhaled reference compound within lung tissue, through the application of MALDI-IMS. The procedure for isolation and preparation of lung tissues is found to be crucial in order to preserve the anatomy and structure of the pulmonary compartments.

To avoid delocalization of analytes within lung tissue compartments we have applied an in-house designed nano-spotter, based on a microdispenser mounted on XY-table, of which movement and spotting functionality were fully computer controlled. We demonstrate the usefulness of this platform in lung tissue sections isolated from rodent in vivo model, applied to compound tissue imaging as exemplified with the determination of the spatial distribution of (1α,2β,4β,7β)-7-[(hydroxidi-2-thienylacetyl)oxy]-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.02,4]nonane, also known as tiotropium. We provide details on tissue preparation protocols and sample spotting technology for successful identification of drug in mouse lung tissue by using MALDI-Orbitrap instrumentation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
microdispenser, rat lung, MALDI Orbitrap, tiotropium, matrix spotting, tissue imaging MALDI-MS
in
Journal of Proteomics2008-01-01+01:00
volume
73
issue
6
pages
1270 - 1278
publisher
Elsevier
external identifiers
  • wos:000277763800023
  • pmid:20193786
  • scopus:77950518892
ISSN
1874-3919
DOI
10.1016/j.jprot.2010.02.021
language
English
LU publication?
yes
id
7d3192f5-dce3-4149-aac6-8deeb859f4e5 (old id 1551021)
date added to LUP
2010-03-04 12:26:30
date last changed
2018-06-24 03:38:30
@article{7d3192f5-dce3-4149-aac6-8deeb859f4e5,
  abstract     = {The ultimate goal of MALDI-Imaging Mass Spectrometry (MALDI-IMS) is to achieve spatial localization of analytes in tissue sections down to individual tissue compartments or even at the level of a few cells. With compound tissue imaging, it is possible to track the transportation of an unlabelled, inhaled reference compound within lung tissue, through the application of MALDI-IMS. The procedure for isolation and preparation of lung tissues is found to be crucial in order to preserve the anatomy and structure of the pulmonary compartments. <br/><br>
To avoid delocalization of analytes within lung tissue compartments we have applied an in-house designed nano-spotter, based on a microdispenser mounted on XY-table, of which movement and spotting functionality were fully computer controlled. We demonstrate the usefulness of this platform in lung tissue sections isolated from rodent in vivo model, applied to compound tissue imaging as exemplified with the determination of the spatial distribution of (1α,2β,4β,7β)-7-[(hydroxidi-2-thienylacetyl)oxy]-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.02,4]nonane, also known as tiotropium. We provide details on tissue preparation protocols and sample spotting technology for successful identification of drug in mouse lung tissue by using MALDI-Orbitrap instrumentation.},
  author       = {Végvári, Ákos and Fehniger, Thomas E. and Gustavsson, Lena and Nilsson, Anna and Andrén, Per E. and Kenne, Kerstin and Nilsson, Johan and Laurell, Thomas and Marko-Varga, György},
  issn         = {1874-3919},
  keyword      = {microdispenser,rat lung,MALDI Orbitrap,tiotropium,matrix spotting,tissue imaging MALDI-MS},
  language     = {eng},
  number       = {6},
  pages        = {1270--1278},
  publisher    = {Elsevier},
  series       = {Journal of Proteomics2008-01-01+01:00},
  title        = {Essential tactics of tissue preparation and matrix nano-spotting for successful compound imaging mass spectrometry},
  url          = {http://dx.doi.org/10.1016/j.jprot.2010.02.021},
  volume       = {73},
  year         = {2010},
}