A food grade approach for the isolation of major alkylresorcinols (ARs) from rye bran applying tailored supercritical carbon dioxide(scCO(2)) extraction combined with HPLC
(2009) In Journal of Supercritical Fluids 51(2). p.167-173- Abstract
- Tailored supercritical carbon dioxide (scCO(2)) extraction of alkyhesorcinols (ARs) from rye bran resulting in pre-purification of ARs already during extraction process. The pre-purification was achieved by splitting the extraction process in 2 steps.. In both steps, the CO2 pressure was set at 25 MPa with a flow rate of 10 g/min. The first step was carried out at 70 degrees C. 0.06% of ethanolic co-solvent for 2 h, followed by the second step at 45,C using 10% of ethanolic co-solvent for 4 h, resulting in a pre-purified ARs extract. From the pre-purified scCO(2) extract, chromatographed on C8 column, were obtained pure ARs homologues of C17, C19 and C21 (68% of total) eluting separately in a linear gradient of ethanol. Additionally, a... (More)
- Tailored supercritical carbon dioxide (scCO(2)) extraction of alkyhesorcinols (ARs) from rye bran resulting in pre-purification of ARs already during extraction process. The pre-purification was achieved by splitting the extraction process in 2 steps.. In both steps, the CO2 pressure was set at 25 MPa with a flow rate of 10 g/min. The first step was carried out at 70 degrees C. 0.06% of ethanolic co-solvent for 2 h, followed by the second step at 45,C using 10% of ethanolic co-solvent for 4 h, resulting in a pre-purified ARs extract. From the pre-purified scCO(2) extract, chromatographed on C8 column, were obtained pure ARs homologues of C17, C19 and C21 (68% of total) eluting separately in a linear gradient of ethanol. Additionally, a rapid method for the isolation of high purified pools of ARs homologues using a disposable solid phase extraction SPE-C18 column and a step gradient of ethanol was developed. (C) 2009 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1567857
- author
- Dey, Estera LU and Mikhailopulo, Konstantin
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Solid phase extraction, dioxide (scCO(2)) HPLC, Supercritical carbon, Rye bran, Purification of alkylresorcinols, Evaporative light, scattering detector
- in
- Journal of Supercritical Fluids
- volume
- 51
- issue
- 2
- pages
- 167 - 173
- publisher
- Elsevier
- external identifiers
-
- wos:000274350800011
- scopus:72049088246
- ISSN
- 0896-8446
- DOI
- 10.1016/j.supflu.2009.09.005
- project
- ANTIDIABETIC FOOD CENTRE
- language
- English
- LU publication?
- yes
- id
- 81c0a1a0-3caf-4f1b-8f6e-e6864d136067 (old id 1567857)
- date added to LUP
- 2016-04-01 12:00:46
- date last changed
- 2022-01-26 21:33:59
@article{81c0a1a0-3caf-4f1b-8f6e-e6864d136067, abstract = {{Tailored supercritical carbon dioxide (scCO(2)) extraction of alkyhesorcinols (ARs) from rye bran resulting in pre-purification of ARs already during extraction process. The pre-purification was achieved by splitting the extraction process in 2 steps.. In both steps, the CO2 pressure was set at 25 MPa with a flow rate of 10 g/min. The first step was carried out at 70 degrees C. 0.06% of ethanolic co-solvent for 2 h, followed by the second step at 45,C using 10% of ethanolic co-solvent for 4 h, resulting in a pre-purified ARs extract. From the pre-purified scCO(2) extract, chromatographed on C8 column, were obtained pure ARs homologues of C17, C19 and C21 (68% of total) eluting separately in a linear gradient of ethanol. Additionally, a rapid method for the isolation of high purified pools of ARs homologues using a disposable solid phase extraction SPE-C18 column and a step gradient of ethanol was developed. (C) 2009 Elsevier B.V. All rights reserved.}}, author = {{Dey, Estera and Mikhailopulo, Konstantin}}, issn = {{0896-8446}}, keywords = {{Solid phase extraction; dioxide (scCO(2)) HPLC; Supercritical carbon; Rye bran; Purification of alkylresorcinols; Evaporative light; scattering detector}}, language = {{eng}}, number = {{2}}, pages = {{167--173}}, publisher = {{Elsevier}}, series = {{Journal of Supercritical Fluids}}, title = {{A food grade approach for the isolation of major alkylresorcinols (ARs) from rye bran applying tailored supercritical carbon dioxide(scCO(2)) extraction combined with HPLC}}, url = {{http://dx.doi.org/10.1016/j.supflu.2009.09.005}}, doi = {{10.1016/j.supflu.2009.09.005}}, volume = {{51}}, year = {{2009}}, }