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A food grade approach for the isolation of major alkylresorcinols (ARs) from rye bran applying tailored supercritical carbon dioxide(scCO(2)) extraction combined with HPLC

Dey, Estera LU and Mikhailopulo, Konstantin (2009) In Journal of Supercritical Fluids 51(2). p.167-173
Abstract
Tailored supercritical carbon dioxide (scCO(2)) extraction of alkyhesorcinols (ARs) from rye bran resulting in pre-purification of ARs already during extraction process. The pre-purification was achieved by splitting the extraction process in 2 steps.. In both steps, the CO2 pressure was set at 25 MPa with a flow rate of 10 g/min. The first step was carried out at 70 degrees C. 0.06% of ethanolic co-solvent for 2 h, followed by the second step at 45,C using 10% of ethanolic co-solvent for 4 h, resulting in a pre-purified ARs extract. From the pre-purified scCO(2) extract, chromatographed on C8 column, were obtained pure ARs homologues of C17, C19 and C21 (68% of total) eluting separately in a linear gradient of ethanol. Additionally, a... (More)
Tailored supercritical carbon dioxide (scCO(2)) extraction of alkyhesorcinols (ARs) from rye bran resulting in pre-purification of ARs already during extraction process. The pre-purification was achieved by splitting the extraction process in 2 steps.. In both steps, the CO2 pressure was set at 25 MPa with a flow rate of 10 g/min. The first step was carried out at 70 degrees C. 0.06% of ethanolic co-solvent for 2 h, followed by the second step at 45,C using 10% of ethanolic co-solvent for 4 h, resulting in a pre-purified ARs extract. From the pre-purified scCO(2) extract, chromatographed on C8 column, were obtained pure ARs homologues of C17, C19 and C21 (68% of total) eluting separately in a linear gradient of ethanol. Additionally, a rapid method for the isolation of high purified pools of ARs homologues using a disposable solid phase extraction SPE-C18 column and a step gradient of ethanol was developed. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
Solid phase extraction, dioxide (scCO(2)) HPLC, Supercritical carbon, Rye bran, Purification of alkylresorcinols, Evaporative light, scattering detector
in
Journal of Supercritical Fluids
volume
51
issue
2
pages
167 - 173
publisher
Elsevier
external identifiers
  • wos:000274350800011
  • scopus:72049088246
ISSN
0896-8446
DOI
10.1016/j.supflu.2009.09.005
language
English
LU publication?
yes
id
81c0a1a0-3caf-4f1b-8f6e-e6864d136067 (old id 1567857)
date added to LUP
2010-03-24 16:26:07
date last changed
2017-07-02 03:36:05
@article{81c0a1a0-3caf-4f1b-8f6e-e6864d136067,
  abstract     = {Tailored supercritical carbon dioxide (scCO(2)) extraction of alkyhesorcinols (ARs) from rye bran resulting in pre-purification of ARs already during extraction process. The pre-purification was achieved by splitting the extraction process in 2 steps.. In both steps, the CO2 pressure was set at 25 MPa with a flow rate of 10 g/min. The first step was carried out at 70 degrees C. 0.06% of ethanolic co-solvent for 2 h, followed by the second step at 45,C using 10% of ethanolic co-solvent for 4 h, resulting in a pre-purified ARs extract. From the pre-purified scCO(2) extract, chromatographed on C8 column, were obtained pure ARs homologues of C17, C19 and C21 (68% of total) eluting separately in a linear gradient of ethanol. Additionally, a rapid method for the isolation of high purified pools of ARs homologues using a disposable solid phase extraction SPE-C18 column and a step gradient of ethanol was developed. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Dey, Estera and Mikhailopulo, Konstantin},
  issn         = {0896-8446},
  keyword      = {Solid phase extraction,dioxide (scCO(2)) HPLC,Supercritical carbon,Rye bran,Purification of alkylresorcinols,Evaporative light,scattering detector},
  language     = {eng},
  number       = {2},
  pages        = {167--173},
  publisher    = {Elsevier},
  series       = {Journal of Supercritical Fluids},
  title        = {A food grade approach for the isolation of major alkylresorcinols (ARs) from rye bran applying tailored supercritical carbon dioxide(scCO(2)) extraction combined with HPLC},
  url          = {http://dx.doi.org/10.1016/j.supflu.2009.09.005},
  volume       = {51},
  year         = {2009},
}