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Optical Touch Pointer for Fluorescence Guided Glioblastoma Resection Using 5-Aminolevulinic Acid

Haj-Hosseini, Neda; Richter, Johan; Andersson-Engels, Stefan LU and Wardell, Karin (2010) In Lasers in Surgery and Medicine 42(1). p.9-14
Abstract
Background and Objective: Total tumor resection in patients with glioblastoma multiforme (GBM) is difficult to achieve due to the tumor's infiltrative way of growing and morphological similarity to the surrounding functioning brain tissue. The diagnosis is usually subjectively performed using a surgical microscope. The objective of this study was to develop and evaluate a hand-held optical touch pointer using a fluorescence spectroscopy system to quantitatively distinguish healthy from malignant brain tissue intraoperatively. Study Design/Materials and Methods: A fluorescence spectroscopy system with pulsed modulation was designed considering optimum energy delivery to the tissue, minimal photobleaching of PpIX and omission of the ambient... (More)
Background and Objective: Total tumor resection in patients with glioblastoma multiforme (GBM) is difficult to achieve due to the tumor's infiltrative way of growing and morphological similarity to the surrounding functioning brain tissue. The diagnosis is usually subjectively performed using a surgical microscope. The objective of this study was to develop and evaluate a hand-held optical touch pointer using a fluorescence spectroscopy system to quantitatively distinguish healthy from malignant brain tissue intraoperatively. Study Design/Materials and Methods: A fluorescence spectroscopy system with pulsed modulation was designed considering optimum energy delivery to the tissue, minimal photobleaching of PpIX and omission of the ambient light background in the operating room (OR). 5-Aminolevulinic acid (5-ALA) of 5 mg/kg body weight was given to the patients with a presumed GBM prior to surgery. During the surgery a laser pulse at 405 nm was delivered to the tissue. PpIX in glioblastoma tumor cells assigned with peaks at 635 and 704 nm was detected using a fiber optical probe. Results/Conclusion: By using the pulsed fluorescence spectroscopy, PpIX fluorescence is quantitatively detected in the GBM. An effective suppression of low power lamp background from the recorded spectra in addition to a significant reduction of high power surgical lights is achieved. Lasers Surg. Med. 42:9-14, 2010. (C) 2010 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
intraoperative, multiforme, glioblastoma, background light suppression, fluorescence spectroscopy
in
Lasers in Surgery and Medicine
volume
42
issue
1
pages
9 - 14
publisher
John Wiley & Sons
external identifiers
  • wos:000274437900002
  • scopus:75749105516
ISSN
0196-8092
DOI
10.1002/lsm.20868
language
English
LU publication?
yes
id
ac0cd5fa-8817-4475-905e-16fc28ef1462 (old id 1568873)
date added to LUP
2010-03-17 13:21:42
date last changed
2018-07-01 04:02:09
@article{ac0cd5fa-8817-4475-905e-16fc28ef1462,
  abstract     = {Background and Objective: Total tumor resection in patients with glioblastoma multiforme (GBM) is difficult to achieve due to the tumor's infiltrative way of growing and morphological similarity to the surrounding functioning brain tissue. The diagnosis is usually subjectively performed using a surgical microscope. The objective of this study was to develop and evaluate a hand-held optical touch pointer using a fluorescence spectroscopy system to quantitatively distinguish healthy from malignant brain tissue intraoperatively. Study Design/Materials and Methods: A fluorescence spectroscopy system with pulsed modulation was designed considering optimum energy delivery to the tissue, minimal photobleaching of PpIX and omission of the ambient light background in the operating room (OR). 5-Aminolevulinic acid (5-ALA) of 5 mg/kg body weight was given to the patients with a presumed GBM prior to surgery. During the surgery a laser pulse at 405 nm was delivered to the tissue. PpIX in glioblastoma tumor cells assigned with peaks at 635 and 704 nm was detected using a fiber optical probe. Results/Conclusion: By using the pulsed fluorescence spectroscopy, PpIX fluorescence is quantitatively detected in the GBM. An effective suppression of low power lamp background from the recorded spectra in addition to a significant reduction of high power surgical lights is achieved. Lasers Surg. Med. 42:9-14, 2010. (C) 2010 Wiley-Liss, Inc.},
  author       = {Haj-Hosseini, Neda and Richter, Johan and Andersson-Engels, Stefan and Wardell, Karin},
  issn         = {0196-8092},
  keyword      = {intraoperative,multiforme,glioblastoma,background light suppression,fluorescence spectroscopy},
  language     = {eng},
  number       = {1},
  pages        = {9--14},
  publisher    = {John Wiley & Sons},
  series       = {Lasers in Surgery and Medicine},
  title        = {Optical Touch Pointer for Fluorescence Guided Glioblastoma Resection Using 5-Aminolevulinic Acid},
  url          = {http://dx.doi.org/10.1002/lsm.20868},
  volume       = {42},
  year         = {2010},
}