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Evidence for disease-regulated transgene expression in the brain with use of lentiviral vectors.

Jakobsson, Johan LU ; Rosenqvist, Nina LU ; Marild, Karl ; V Agoston, Denes and Lundberg, Cecilia LU (2006) In Journal of Neuroscience Research 84(1). p.58-67
Abstract
In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined... (More)
In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined regulation and targeting. This disease-regulated approach allows control of transgene expression in the brain without the use of inducer molecules and without overexpression of transactivator proteins (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
disease-regulated transgene expression, brain, lentiviral vectors
in
Journal of Neuroscience Research
volume
84
issue
1
pages
58 - 67
publisher
John Wiley and Sons Inc.
external identifiers
  • pmid:16683227
  • wos:000238897400006
  • scopus:33745785622
ISSN
1097-4547
DOI
10.1002/jnr.20872
language
English
LU publication?
yes
id
1f6b729b-b841-41e0-901c-54e0af801511 (old id 156910)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16683227&dopt=Abstract
date added to LUP
2016-04-01 17:15:29
date last changed
2020-03-24 04:43:54
@article{1f6b729b-b841-41e0-901c-54e0af801511,
  abstract     = {In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined regulation and targeting. This disease-regulated approach allows control of transgene expression in the brain without the use of inducer molecules and without overexpression of transactivator proteins},
  author       = {Jakobsson, Johan and Rosenqvist, Nina and Marild, Karl and V Agoston, Denes and Lundberg, Cecilia},
  issn         = {1097-4547},
  language     = {eng},
  number       = {1},
  pages        = {58--67},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Journal of Neuroscience Research},
  title        = {Evidence for disease-regulated transgene expression in the brain with use of lentiviral vectors.},
  url          = {http://dx.doi.org/10.1002/jnr.20872},
  doi          = {10.1002/jnr.20872},
  volume       = {84},
  year         = {2006},
}