Evidence for disease-regulated transgene expression in the brain with use of lentiviral vectors.
Jakobsson, Johan LU
; Rosenqvist, Nina
LU
; Marild, Karl
; V Agoston, Denes
and Lundberg, Cecilia
LU
(2006)
In Journal of Neuroscience Research
84(1).
p.58-67
- Abstract
- In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined... (More)
- In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined regulation and targeting. This disease-regulated approach allows control of transgene expression in the brain without the use of inducer molecules and without overexpression of transactivator proteins (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/156910
- author
- Jakobsson, Johan
LU
; Rosenqvist, Nina
LU
; Marild, Karl
; V Agoston, Denes
and Lundberg, Cecilia
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- disease-regulated transgene expression, brain, lentiviral vectors
- in
- Journal of Neuroscience Research
- volume
- 84
- issue
- 1
- pages
- 58 - 67
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:16683227
- wos:000238897400006
- scopus:33745785622
- ISSN
- 1097-4547
- DOI
- 10.1002/jnr.20872
- language
- English
- LU publication?
- yes
- id
- 1f6b729b-b841-41e0-901c-54e0af801511 (old id 156910)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16683227&dopt=Abstract
- date added to LUP
- 2016-04-01 17:15:29
- date last changed
- 2022-03-23 00:27:29
@article{1f6b729b-b841-41e0-901c-54e0af801511,
abstract = {{In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined regulation and targeting. This disease-regulated approach allows control of transgene expression in the brain without the use of inducer molecules and without overexpression of transactivator proteins}},
author = {{Jakobsson, Johan and Rosenqvist, Nina and Marild, Karl and V Agoston, Denes and Lundberg, Cecilia}},
issn = {{1097-4547}},
keywords = {{disease-regulated transgene expression; brain; lentiviral vectors}},
language = {{eng}},
number = {{1}},
pages = {{58--67}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Neuroscience Research}},
title = {{Evidence for disease-regulated transgene expression in the brain with use of lentiviral vectors.}},
url = {{http://dx.doi.org/10.1002/jnr.20872}},
doi = {{10.1002/jnr.20872}},
volume = {{84}},
year = {{2006}},
}