Immobilisation and application of lipases in organic media.
Adlercreutz, Patrick LU
(2013)
In Chemical Society Reviews
42(15).
p.6406-6436
- Abstract
- Different methods of preparing lipases for use in organic media are critically reviewed. Solid lipase preparations can be made by typical immobilisation methods such as adsorption, entrapment, covalent coupling or cross-linking. Immobilisation is especially attractive for lipases because, in addition to the normal benefits of enzyme immobilisation, it can also lead to a considerable increase in catalytic activity, probably caused by conformational changes in the lipase molecules. Activation can be achieved, for example, using hydrophobic support materials or surfactants during the immobilisation procedure. Surfactants can also be used to solubilise lipases in organic media via the formation of hydrophobic ion pairs, surfactant-coated... (More)
- Different methods of preparing lipases for use in organic media are critically reviewed. Solid lipase preparations can be made by typical immobilisation methods such as adsorption, entrapment, covalent coupling or cross-linking. Immobilisation is especially attractive for lipases because, in addition to the normal benefits of enzyme immobilisation, it can also lead to a considerable increase in catalytic activity, probably caused by conformational changes in the lipase molecules. Activation can be achieved, for example, using hydrophobic support materials or surfactants during the immobilisation procedure. Surfactants can also be used to solubilise lipases in organic media via the formation of hydrophobic ion pairs, surfactant-coated lipase or reversed micelles. Lipase preparation methods are discussed with regard to potential lipase inactivation and activation effects, mass transfer limitations, lipase stability and other features important for applications. The practical applications of lipases in organic media reviewed include ester synthesis, modification of triacylglycerols and phospholipids, fatty acid enrichment, enantiomer resolution, biodiesel production and acylation of carbohydrates and bioactive compounds. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3559862
- author
- Adlercreutz, Patrick
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Chemical Society Reviews
- volume
- 42
- issue
- 15
- pages
- 6406 - 6436
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000321570200013
- pmid:23403895
- scopus:84880105945
- pmid:23403895
- ISSN
- 0306-0012
- DOI
- 10.1039/c3cs35446f
- language
- English
- LU publication?
- yes
- id
- 156ef714-61ac-4d10-9cf8-659787e840a5 (old id 3559862)
- date added to LUP
- 2016-04-01 10:01:45
- date last changed
- 2022-04-27 17:34:43
@article{156ef714-61ac-4d10-9cf8-659787e840a5,
abstract = {{Different methods of preparing lipases for use in organic media are critically reviewed. Solid lipase preparations can be made by typical immobilisation methods such as adsorption, entrapment, covalent coupling or cross-linking. Immobilisation is especially attractive for lipases because, in addition to the normal benefits of enzyme immobilisation, it can also lead to a considerable increase in catalytic activity, probably caused by conformational changes in the lipase molecules. Activation can be achieved, for example, using hydrophobic support materials or surfactants during the immobilisation procedure. Surfactants can also be used to solubilise lipases in organic media via the formation of hydrophobic ion pairs, surfactant-coated lipase or reversed micelles. Lipase preparation methods are discussed with regard to potential lipase inactivation and activation effects, mass transfer limitations, lipase stability and other features important for applications. The practical applications of lipases in organic media reviewed include ester synthesis, modification of triacylglycerols and phospholipids, fatty acid enrichment, enantiomer resolution, biodiesel production and acylation of carbohydrates and bioactive compounds.}},
author = {{Adlercreutz, Patrick}},
issn = {{0306-0012}},
language = {{eng}},
number = {{15}},
pages = {{6406--6436}},
publisher = {{Royal Society of Chemistry}},
series = {{Chemical Society Reviews}},
title = {{Immobilisation and application of lipases in organic media.}},
url = {{http://dx.doi.org/10.1039/c3cs35446f}},
doi = {{10.1039/c3cs35446f}},
volume = {{42}},
year = {{2013}},
}