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The interaction between DNA and cationic lipid films at the air-water interface

Cárdenas, Marité LU ; Nylander, Tommy LU ; Jönsson, Bengt LU and Lindman, Björn LU (2005) In Journal of Colloid and Interface Science 286(1). p.166-175
Abstract
The interaction between DNA and positively charged dioctadecyldimethylammonium bromide (DODAB) and DODAB/disteroylphosphatidylcholine (DSPC) monolayers at the air-aqueous interface was studied by a combination of the surface film balance and Brewster angle microscopy. In presence of DNA, the &UPi;-A isotherm of the cationic monolayer shifts to larger mean molecular areas due to the electrostatic interaction with DNA while the typical liquid expanded-liquid condensed phase transition for DODAB monolayers disappear and the monolayer remains to be in the liquid expanded phase. Furthermore, the morphology of the film dramatically changes, where the large dendritic-like condensed aggregates observed for DODAB monolayers vanish. The charge... (More)
The interaction between DNA and positively charged dioctadecyldimethylammonium bromide (DODAB) and DODAB/disteroylphosphatidylcholine (DSPC) monolayers at the air-aqueous interface was studied by a combination of the surface film balance and Brewster angle microscopy. In presence of DNA, the &UPi;-A isotherm of the cationic monolayer shifts to larger mean molecular areas due to the electrostatic interaction with DNA while the typical liquid expanded-liquid condensed phase transition for DODAB monolayers disappear and the monolayer remains to be in the liquid expanded phase. Furthermore, the morphology of the film dramatically changes, where the large dendritic-like condensed aggregates observed for DODAB monolayers vanish. The charge density of the monolayer was varied by using mixed monolayers with the zwitterionic DSPC and no large effect was observed on the interaction with DNA. By modeling the electrostatic interactions with the linearized Poisson-Boltzmann equation using the finite-element method and taking into account the assumption in the dielectric constants of the system, it was possible to corroborate the expansion of the cationic monolayer upon interaction with DNA as well as the fact that DNA does not seem to penetrate into the monolayer. © 2005 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Colloid and Interface Science
volume
286
issue
1
pages
166 - 175
publisher
Elsevier
external identifiers
  • wos:000228935700019
  • scopus:17644380292
ISSN
1095-7103
DOI
10.1016/j.jcis.2005.01.008
language
English
LU publication?
yes
id
ee448148-5cbc-4145-be06-23c95870d0fe (old id 157248)
date added to LUP
2007-07-11 08:39:41
date last changed
2017-07-09 03:47:42
@article{ee448148-5cbc-4145-be06-23c95870d0fe,
  abstract     = {The interaction between DNA and positively charged dioctadecyldimethylammonium bromide (DODAB) and DODAB/disteroylphosphatidylcholine (DSPC) monolayers at the air-aqueous interface was studied by a combination of the surface film balance and Brewster angle microscopy. In presence of DNA, the &UPi;-A isotherm of the cationic monolayer shifts to larger mean molecular areas due to the electrostatic interaction with DNA while the typical liquid expanded-liquid condensed phase transition for DODAB monolayers disappear and the monolayer remains to be in the liquid expanded phase. Furthermore, the morphology of the film dramatically changes, where the large dendritic-like condensed aggregates observed for DODAB monolayers vanish. The charge density of the monolayer was varied by using mixed monolayers with the zwitterionic DSPC and no large effect was observed on the interaction with DNA. By modeling the electrostatic interactions with the linearized Poisson-Boltzmann equation using the finite-element method and taking into account the assumption in the dielectric constants of the system, it was possible to corroborate the expansion of the cationic monolayer upon interaction with DNA as well as the fact that DNA does not seem to penetrate into the monolayer. © 2005 Elsevier Inc. All rights reserved.},
  author       = {Cárdenas, Marité and Nylander, Tommy and Jönsson, Bengt and Lindman, Björn},
  issn         = {1095-7103},
  language     = {eng},
  number       = {1},
  pages        = {166--175},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {The interaction between DNA and cationic lipid films at the air-water interface},
  url          = {http://dx.doi.org/10.1016/j.jcis.2005.01.008},
  volume       = {286},
  year         = {2005},
}