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Interaction between DNA and charged colloids could be hydrophobically driven

Cárdenas, Marité LU ; Schillén, Karin LU ; Pebalk, Dmitri LU ; Nylander, Tommy LU and Lindman, Björn LU (2005) In Biomacromolecules 6(2). p.832-837
Abstract
The interaction of DNA with amino-functionalized polystyrene particles has been studied by using a dynamic light scattering (DLS) technique. In 10 mM NaBr solution the particles have a hydrodynamic radius of 76 nm and the DNA macromolecule investigated (double stranded) has a hydrodynamic radius of 107 nm. At very low DNA concentrations, DNA adopts a flat conformation on the particle surface. If the DNA concentration is increased above 0.1 mu g/mL, the thickness of the DNA layer increases, suggesting the presence of large loops and tails. Although the particles contain primary amino groups, they have a negative net charge under the conditions used in this work. Thus, the driving force for DNA adsorption is not of electrostatic origin but... (More)
The interaction of DNA with amino-functionalized polystyrene particles has been studied by using a dynamic light scattering (DLS) technique. In 10 mM NaBr solution the particles have a hydrodynamic radius of 76 nm and the DNA macromolecule investigated (double stranded) has a hydrodynamic radius of 107 nm. At very low DNA concentrations, DNA adopts a flat conformation on the particle surface. If the DNA concentration is increased above 0.1 mu g/mL, the thickness of the DNA layer increases, suggesting the presence of large loops and tails. Although the particles contain primary amino groups, they have a negative net charge under the conditions used in this work. Thus, the driving force for DNA adsorption is not of electrostatic origin but rather due to a hydrophobic effect. Addition of cationic surfactant to the DNA-precoated amino functionalized particles induces changes in the adsorbed layer conformation, in agreement with the coadsorption of cationic surfactant. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
6
issue
2
pages
832 - 837
publisher
The American Chemical Society
external identifiers
  • wos:000227687800041
  • scopus:16344372105
ISSN
1526-4602
DOI
10.1021/bm049422h
language
English
LU publication?
yes
id
c52727d9-d60d-45ec-9a05-58441eabb8b1 (old id 157265)
date added to LUP
2007-07-11 08:47:28
date last changed
2017-01-01 04:29:22
@article{c52727d9-d60d-45ec-9a05-58441eabb8b1,
  abstract     = {The interaction of DNA with amino-functionalized polystyrene particles has been studied by using a dynamic light scattering (DLS) technique. In 10 mM NaBr solution the particles have a hydrodynamic radius of 76 nm and the DNA macromolecule investigated (double stranded) has a hydrodynamic radius of 107 nm. At very low DNA concentrations, DNA adopts a flat conformation on the particle surface. If the DNA concentration is increased above 0.1 mu g/mL, the thickness of the DNA layer increases, suggesting the presence of large loops and tails. Although the particles contain primary amino groups, they have a negative net charge under the conditions used in this work. Thus, the driving force for DNA adsorption is not of electrostatic origin but rather due to a hydrophobic effect. Addition of cationic surfactant to the DNA-precoated amino functionalized particles induces changes in the adsorbed layer conformation, in agreement with the coadsorption of cationic surfactant.},
  author       = {Cárdenas, Marité and Schillén, Karin and Pebalk, Dmitri and Nylander, Tommy and Lindman, Björn},
  issn         = {1526-4602},
  language     = {eng},
  number       = {2},
  pages        = {832--837},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Interaction between DNA and charged colloids could be hydrophobically driven},
  url          = {http://dx.doi.org/10.1021/bm049422h},
  volume       = {6},
  year         = {2005},
}