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Enzymatic degradation of model cellulose films

Eriksson, Jonny; Malmsten, M; Tiberg, Fredrik LU ; Callisen, T H; Damhus, T and Johansen, K S (2005) In Journal of Colloid and Interface Science 284(1). p.99-106
Abstract
Enzymatic degradation of model cellulose films prepared by a spin-coating technique was investigated by ellipsometry. The cellulose films were prior to degradation characterized by ellipsometry, contact angle measurements, ESCA (electron spectroscopy for chemical analysis) and AFM (atomic force microscopy). At enzyme addition to preformed cellulose films an initial adsorption was observed, which was followed by a total interfacial mass decrease due to enzymatic degradation of the cellulose films. The degradation rate was found to be constant during an extended time of hours, whereafter the degradation leveled off. In parallel to the decreased interfacial mass, the cellulose degradation resulted in a thinner and more dilute interfacial... (More)
Enzymatic degradation of model cellulose films prepared by a spin-coating technique was investigated by ellipsometry. The cellulose films were prior to degradation characterized by ellipsometry, contact angle measurements, ESCA (electron spectroscopy for chemical analysis) and AFM (atomic force microscopy). At enzyme addition to preformed cellulose films an initial adsorption was observed, which was followed by a total interfacial mass decrease due to enzymatic degradation of the cellulose films. The degradation rate was found to be constant during an extended time of hours, whereafter the degradation leveled off. In parallel to the decreased interfacial mass, the cellulose degradation resulted in a thinner and more dilute interfacial film. At long degradation times, however, there was an expansion of the cellulose film. The enzyme concentration affected the degradation rate significantly, with a faster degradation at a higher enzyme concentration. The effects of pH, temperature, ionic strength and stirring rate in the cuvette were also investigated. (c) 2004 Elsevier Inc. All rights reserved. (Less)
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type
Contribution to journal
publication status
published
subject
in
Journal of Colloid and Interface Science
volume
284
issue
1
pages
99 - 106
publisher
Elsevier
external identifiers
  • pmid:15752790
  • wos:000227819700014
  • scopus:14844303745
ISSN
1095-7103
DOI
10.1016/j.jcis.2004.10.041
language
English
LU publication?
yes
id
d9101812-33fa-40c4-becf-13f1ae8290a6 (old id 157364)
date added to LUP
2007-07-11 11:21:54
date last changed
2017-11-05 03:30:54
@article{d9101812-33fa-40c4-becf-13f1ae8290a6,
  abstract     = {Enzymatic degradation of model cellulose films prepared by a spin-coating technique was investigated by ellipsometry. The cellulose films were prior to degradation characterized by ellipsometry, contact angle measurements, ESCA (electron spectroscopy for chemical analysis) and AFM (atomic force microscopy). At enzyme addition to preformed cellulose films an initial adsorption was observed, which was followed by a total interfacial mass decrease due to enzymatic degradation of the cellulose films. The degradation rate was found to be constant during an extended time of hours, whereafter the degradation leveled off. In parallel to the decreased interfacial mass, the cellulose degradation resulted in a thinner and more dilute interfacial film. At long degradation times, however, there was an expansion of the cellulose film. The enzyme concentration affected the degradation rate significantly, with a faster degradation at a higher enzyme concentration. The effects of pH, temperature, ionic strength and stirring rate in the cuvette were also investigated. (c) 2004 Elsevier Inc. All rights reserved.},
  author       = {Eriksson, Jonny and Malmsten, M and Tiberg, Fredrik and Callisen, T H and Damhus, T and Johansen, K S},
  issn         = {1095-7103},
  language     = {eng},
  number       = {1},
  pages        = {99--106},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Enzymatic degradation of model cellulose films},
  url          = {http://dx.doi.org/10.1016/j.jcis.2004.10.041},
  volume       = {284},
  year         = {2005},
}