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Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions

McLoughlin, D; Dias, R; Lindman, Björn LU ; Cardenas, M; Nylander, Tommy LU ; Dawson, K; Miguel, M and Langevin, D (2005) In Langmuir 21(5). p.1900-1907
Abstract
DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded-liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA... (More)
DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded-liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
21
issue
5
pages
1900 - 1907
publisher
The American Chemical Society
external identifiers
  • pmid:15723487
  • wos:000227193500037
  • scopus:14844336901
ISSN
0743-7463
DOI
10.1021/la047700s
language
English
LU publication?
yes
id
c5faf7a4-29ef-4574-bb89-6708c52d0ad5 (old id 157477)
date added to LUP
2007-07-11 16:23:35
date last changed
2017-10-01 03:58:53
@article{c5faf7a4-29ef-4574-bb89-6708c52d0ad5,
  abstract     = {DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded-liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies.},
  author       = {McLoughlin, D and Dias, R and Lindman, Björn and Cardenas, M and Nylander, Tommy and Dawson, K and Miguel, M and Langevin, D},
  issn         = {0743-7463},
  language     = {eng},
  number       = {5},
  pages        = {1900--1907},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions},
  url          = {http://dx.doi.org/10.1021/la047700s},
  volume       = {21},
  year         = {2005},
}