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Retrovirally delivered Islet-1 increases recruitment of Ng2 expressing cells from the postnatal SVZ into the striatum.

Rogelius, Nina LU ; Hebsgaard, Josephine LU ; Lundberg, Cecilia LU and Parmar, Malin LU (2006) In Experimental Neurology 201(Jun 23). p.388-398
Abstract
Neural stem and progenitor cells hold the promise to be used in cell-based therapies to treat both acute and degenerative neurological diseases. To date, most research has been focused on the use of in vitro propagated stem cells used as a source of cells in cell replacement therapies. However, mobilization of endogenous neural stem cells to generate a specific differentiated cell type offers an attractive alternative. In this study, we investigate the possibility to direct the formation of specific cells from the endogenous stem and progenitor cells residing in the subventricular region of the postnatal brain. With the aim to induce postnatal generation of striatal neurons, we ectopically expressed Islet-1, a LIM homeodomain transcription... (More)
Neural stem and progenitor cells hold the promise to be used in cell-based therapies to treat both acute and degenerative neurological diseases. To date, most research has been focused on the use of in vitro propagated stem cells used as a source of cells in cell replacement therapies. However, mobilization of endogenous neural stem cells to generate a specific differentiated cell type offers an attractive alternative. In this study, we investigate the possibility to direct the formation of specific cells from the endogenous stem and progenitor cells residing in the subventricular region of the postnatal brain. With the aim to induce postnatal generation of striatal neurons, we ectopically expressed Islet-1, a LIM homeodomain transcription factor expressed by striatal progenitors during development, in cells of the subventricular zone (SVZ) of neonatal and adult rats. Ectopic expression of Islet-1 in the neonatal, but not adult, SVZ resulted in the appearance of a population of cells in the striatum. These cells were primarily located in the ventrolateral area of the striatum where they differentiate into Ng2 expressing cells. However, no neurogenesis was observed in the striatum, nor was ectopic striatal differentiation observed in any other area of the brain after retroviral expression of Islet-1 in the SVZ. Thus, although ectopic expression of Islet-1 is sufficient to direct the migration of cells into the striatum in neonatal animals, it does not specify a striatal projection neuron phenotype in cells generated from the SVZ after birth. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
endogenous stem cells, GFP, subventricular zone, rat, striatum
in
Experimental Neurology
volume
201
issue
Jun 23
pages
388 - 398
publisher
Academic Press
external identifiers
  • wos:000241393900013
  • scopus:33748430181
  • pmid:16806175
ISSN
0014-4886
DOI
10.1016/j.expneurol.2006.04.022
language
English
LU publication?
yes
id
a9e75e7a-36cc-4bed-ba53-acb27f9843d6 (old id 158054)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16806175&dopt=Abstract
date added to LUP
2016-04-01 11:52:44
date last changed
2020-01-12 08:47:09
@article{a9e75e7a-36cc-4bed-ba53-acb27f9843d6,
  abstract     = {Neural stem and progenitor cells hold the promise to be used in cell-based therapies to treat both acute and degenerative neurological diseases. To date, most research has been focused on the use of in vitro propagated stem cells used as a source of cells in cell replacement therapies. However, mobilization of endogenous neural stem cells to generate a specific differentiated cell type offers an attractive alternative. In this study, we investigate the possibility to direct the formation of specific cells from the endogenous stem and progenitor cells residing in the subventricular region of the postnatal brain. With the aim to induce postnatal generation of striatal neurons, we ectopically expressed Islet-1, a LIM homeodomain transcription factor expressed by striatal progenitors during development, in cells of the subventricular zone (SVZ) of neonatal and adult rats. Ectopic expression of Islet-1 in the neonatal, but not adult, SVZ resulted in the appearance of a population of cells in the striatum. These cells were primarily located in the ventrolateral area of the striatum where they differentiate into Ng2 expressing cells. However, no neurogenesis was observed in the striatum, nor was ectopic striatal differentiation observed in any other area of the brain after retroviral expression of Islet-1 in the SVZ. Thus, although ectopic expression of Islet-1 is sufficient to direct the migration of cells into the striatum in neonatal animals, it does not specify a striatal projection neuron phenotype in cells generated from the SVZ after birth. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Rogelius, Nina and Hebsgaard, Josephine and Lundberg, Cecilia and Parmar, Malin},
  issn         = {0014-4886},
  language     = {eng},
  number       = {Jun 23},
  pages        = {388--398},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Retrovirally delivered Islet-1 increases recruitment of Ng2 expressing cells from the postnatal SVZ into the striatum.},
  url          = {http://dx.doi.org/10.1016/j.expneurol.2006.04.022},
  doi          = {10.1016/j.expneurol.2006.04.022},
  volume       = {201},
  year         = {2006},
}