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An easy-to-use method for detecting fetal hemoglobin-A test to identify bleeding from vasa previa.

Lindqvist, Pelle and Gren, Peter (2006) In European Journal of Obstetrics, Gynecology, and Reproductive Biology 131(2). p.151-153
Abstract
Background: Vasa previa is a rare but potentially dangerous fetal condition that may occur during pregnancy. Ideally, all cases such cases are detected antenatally, but many present as late vaginal hemorrhaging. At the current time, there is no test for fetal hemoglobin (HbF) in general use. Methods: A modified method of identifying HbF is presented. Five milliliters of 0.14 M NaOH was combined with 50 mu l of a mixture of fetal and maternal blood. After 2 min, it was judged if the solution still had a red tone or not. The sensitivity of this method for detecting HbF was assessed. Results: All 15 clinical personnel could identify both 69% and 34% HbF mixed with adult hemoglobin (100% sensitivity), 14 out of 15 could identify 17% HbF (93%... (More)
Background: Vasa previa is a rare but potentially dangerous fetal condition that may occur during pregnancy. Ideally, all cases such cases are detected antenatally, but many present as late vaginal hemorrhaging. At the current time, there is no test for fetal hemoglobin (HbF) in general use. Methods: A modified method of identifying HbF is presented. Five milliliters of 0.14 M NaOH was combined with 50 mu l of a mixture of fetal and maternal blood. After 2 min, it was judged if the solution still had a red tone or not. The sensitivity of this method for detecting HbF was assessed. Results: All 15 clinical personnel could identify both 69% and 34% HbF mixed with adult hemoglobin (100% sensitivity), 14 out of 15 could identify 17% HbF (93% sensitivity), and 12 out of 15 could identify a mixture containing 8% HbF (80% sensitivity). Conclusion: Our rapid. simple test for HbF was at least as sensitive as slower, more cumbersome alkali denaturation tests in common use. It could prove to be a lifesaving toot in ruling out vasa previa bleeding in cases of unclear late pregnancy hemorrhages. (Less)
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type
Contribution to journal
publication status
published
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in
European Journal of Obstetrics, Gynecology, and Reproductive Biology
volume
131
issue
2
pages
151 - 153
publisher
Elsevier
external identifiers
  • scopus:33947397193
  • pmid:16797824
ISSN
0301-2115
DOI
10.1016/j.ejogrb.2006.05.011
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pediatrics/Urology/Gynecology/Endocrinology (013240400)
id
4c4df69b-f402-40af-a2cc-f4df3ae8d4e2 (old id 158132)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16797824&dopt=Abstract
date added to LUP
2016-04-01 12:14:12
date last changed
2021-06-02 01:43:49
@article{4c4df69b-f402-40af-a2cc-f4df3ae8d4e2,
  abstract     = {Background: Vasa previa is a rare but potentially dangerous fetal condition that may occur during pregnancy. Ideally, all cases such cases are detected antenatally, but many present as late vaginal hemorrhaging. At the current time, there is no test for fetal hemoglobin (HbF) in general use. Methods: A modified method of identifying HbF is presented. Five milliliters of 0.14 M NaOH was combined with 50 mu l of a mixture of fetal and maternal blood. After 2 min, it was judged if the solution still had a red tone or not. The sensitivity of this method for detecting HbF was assessed. Results: All 15 clinical personnel could identify both 69% and 34% HbF mixed with adult hemoglobin (100% sensitivity), 14 out of 15 could identify 17% HbF (93% sensitivity), and 12 out of 15 could identify a mixture containing 8% HbF (80% sensitivity). Conclusion: Our rapid. simple test for HbF was at least as sensitive as slower, more cumbersome alkali denaturation tests in common use. It could prove to be a lifesaving toot in ruling out vasa previa bleeding in cases of unclear late pregnancy hemorrhages.},
  author       = {Lindqvist, Pelle and Gren, Peter},
  issn         = {0301-2115},
  language     = {eng},
  number       = {2},
  pages        = {151--153},
  publisher    = {Elsevier},
  series       = {European Journal of Obstetrics, Gynecology, and Reproductive Biology},
  title        = {An easy-to-use method for detecting fetal hemoglobin-A test to identify bleeding from vasa previa.},
  url          = {https://lup.lub.lu.se/search/ws/files/2839568/1059988.pdf},
  doi          = {10.1016/j.ejogrb.2006.05.011},
  volume       = {131},
  year         = {2006},
}