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Isolation and generation of neurosphere cultures from embryonic and adult mouse brain.

Ahlenius, Henrik LU and Kokaia, Zaal LU (2010) In Methods in Molecular Biology 633. p.241-252
Abstract
Neural stem cells are defined as cells that either gives rise to or derives from the cells of the central nervous system and have the unique properties of stem cells, i.e. self-renewal and multipotentiality. One of the widely used methods of expanding neural stem cells under culture conditions is based on the capacity of these cells to divide continuously when cultured in serum-free medium supplemented with various growth factors. One common method used is to grow neural stem cells as free-floating aggregates of cells called neurospheres. Neurospheres can be generated from several structures of the embryonic and adult mammalian brain. Although viable lines can be generated from crude extracts of brain, a precise dissection is crucial to... (More)
Neural stem cells are defined as cells that either gives rise to or derives from the cells of the central nervous system and have the unique properties of stem cells, i.e. self-renewal and multipotentiality. One of the widely used methods of expanding neural stem cells under culture conditions is based on the capacity of these cells to divide continuously when cultured in serum-free medium supplemented with various growth factors. One common method used is to grow neural stem cells as free-floating aggregates of cells called neurospheres. Neurospheres can be generated from several structures of the embryonic and adult mammalian brain. Although viable lines can be generated from crude extracts of brain, a precise dissection is crucial to get a pure population of cells. Here we describe methods for dissection, isolation and generation of neurospheres from embryonic ganglionic eminences and adult subventricular zone of mice and rats. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
633
pages
241 - 252
publisher
Springer
external identifiers
  • pmid:20204633
  • scopus:77953013554
ISSN
1940-6029
DOI
10.1007/978-1-59745-019-5_18
language
English
LU publication?
yes
id
53b24b4c-bee0-414a-bc7f-b849a5bd64b6 (old id 1582540)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20204633?dopt=Abstract
date added to LUP
2010-04-07 13:52:13
date last changed
2018-06-17 04:57:17
@article{53b24b4c-bee0-414a-bc7f-b849a5bd64b6,
  abstract     = {Neural stem cells are defined as cells that either gives rise to or derives from the cells of the central nervous system and have the unique properties of stem cells, i.e. self-renewal and multipotentiality. One of the widely used methods of expanding neural stem cells under culture conditions is based on the capacity of these cells to divide continuously when cultured in serum-free medium supplemented with various growth factors. One common method used is to grow neural stem cells as free-floating aggregates of cells called neurospheres. Neurospheres can be generated from several structures of the embryonic and adult mammalian brain. Although viable lines can be generated from crude extracts of brain, a precise dissection is crucial to get a pure population of cells. Here we describe methods for dissection, isolation and generation of neurospheres from embryonic ganglionic eminences and adult subventricular zone of mice and rats.},
  author       = {Ahlenius, Henrik and Kokaia, Zaal},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {241--252},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Isolation and generation of neurosphere cultures from embryonic and adult mouse brain.},
  url          = {http://dx.doi.org/10.1007/978-1-59745-019-5_18},
  volume       = {633},
  year         = {2010},
}