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Elevated neutrophil, macrophage and dendritic cell numbers characterize immune cell populations in mice chronically infected with Salmonella.

Johansson, Cecilia LU ; Ingman, Mikael LU and Wick, Mary Jo LU (2006) In Microbial Pathogenesis 41(2-3). p.49-58
Abstract
The present study characterizes immune cell populations in mice chronically infected with Salmonella. Mice were characterized as chronically infected based on persistently high titers of Salmonella-reactive immunoglobulins in the serum > 6 months after a single oral dose of S. enterica serovar Typhimurium. These mice had a visibly enlarged spleen but not liver, while both organs harbored bacteria and had increased total cellularity up to 11 months post-infection. Flow cytometry analysis revealed significantly elevated numbers of neutrophils, dendritic cells (DC) and macrophages in the spleen of chronically infected mice. In contrast, no significant increase in the absolute number of T and B cells was apparent in the spleen and DX5(+)... (More)
The present study characterizes immune cell populations in mice chronically infected with Salmonella. Mice were characterized as chronically infected based on persistently high titers of Salmonella-reactive immunoglobulins in the serum > 6 months after a single oral dose of S. enterica serovar Typhimurium. These mice had a visibly enlarged spleen but not liver, while both organs harbored bacteria and had increased total cellularity up to 11 months post-infection. Flow cytometry analysis revealed significantly elevated numbers of neutrophils, dendritic cells (DC) and macrophages in the spleen of chronically infected mice. In contrast, no significant increase in the absolute number of T and B cells was apparent in the spleen and DX5(+) cells, which includes NK cells, some NK T cells and possibly some activated T cells, appears to correlate with chronic Salmonella infection in the liver but not the spleen. In situ analyses revealed that CD8(alpha)(+) DC and Gr-1(+) cells (neutrophils) increased in the splenic red pulp of chronically infected mice. In addition,,Gr-1(+) cells, CD68(+) cells and CD11c(+) cells (DC), the latter lacking detectable staining for CD8 alpha and CD4, accumulated around hepatic blood vessels and in the hepatic network in the liver of mice chronically harboring bacteria. These data provide insight into changes that occur within immune cell populations, most notably within splenic and hepatic phagocytic cell populations, that accompany chronic infection with the intracellular bacterium Salmonella. (c) 2006 Elsevier Ltd. All rights reserved. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
dendritic cell, infection, salmonella, macrophage, lymphocytes
in
Microbial Pathogenesis
volume
41
issue
2-3
pages
49 - 58
publisher
Academic Press
external identifiers
  • wos:000240369800001
  • scopus:33747129192
  • pmid:16782300
ISSN
1096-1208
DOI
10.1016/j.micpath.2006.03.004
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division III (013230700), Immunology (013212020), Division of Infection Medicine (SUS) (013008000)
id
e2777240-0158-4d0d-bced-9fe56e2cf874 (old id 158268)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16782300&dopt=Abstract
date added to LUP
2016-04-01 12:21:22
date last changed
2021-08-25 04:20:14
@article{e2777240-0158-4d0d-bced-9fe56e2cf874,
  abstract     = {The present study characterizes immune cell populations in mice chronically infected with Salmonella. Mice were characterized as chronically infected based on persistently high titers of Salmonella-reactive immunoglobulins in the serum > 6 months after a single oral dose of S. enterica serovar Typhimurium. These mice had a visibly enlarged spleen but not liver, while both organs harbored bacteria and had increased total cellularity up to 11 months post-infection. Flow cytometry analysis revealed significantly elevated numbers of neutrophils, dendritic cells (DC) and macrophages in the spleen of chronically infected mice. In contrast, no significant increase in the absolute number of T and B cells was apparent in the spleen and DX5(+) cells, which includes NK cells, some NK T cells and possibly some activated T cells, appears to correlate with chronic Salmonella infection in the liver but not the spleen. In situ analyses revealed that CD8(alpha)(+) DC and Gr-1(+) cells (neutrophils) increased in the splenic red pulp of chronically infected mice. In addition,,Gr-1(+) cells, CD68(+) cells and CD11c(+) cells (DC), the latter lacking detectable staining for CD8 alpha and CD4, accumulated around hepatic blood vessels and in the hepatic network in the liver of mice chronically harboring bacteria. These data provide insight into changes that occur within immune cell populations, most notably within splenic and hepatic phagocytic cell populations, that accompany chronic infection with the intracellular bacterium Salmonella. (c) 2006 Elsevier Ltd. All rights reserved.},
  author       = {Johansson, Cecilia and Ingman, Mikael and Wick, Mary Jo},
  issn         = {1096-1208},
  language     = {eng},
  number       = {2-3},
  pages        = {49--58},
  publisher    = {Academic Press},
  series       = {Microbial Pathogenesis},
  title        = {Elevated neutrophil, macrophage and dendritic cell numbers characterize immune cell populations in mice chronically infected with Salmonella.},
  url          = {http://dx.doi.org/10.1016/j.micpath.2006.03.004},
  doi          = {10.1016/j.micpath.2006.03.004},
  volume       = {41},
  year         = {2006},
}