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A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by PamCSK, R-837 and CpG-2006 stimulation.

Månsson, Anne LU ; Adner, Mikael LU ; Höckerfelt, Ulf LU and Cardell, Lars-Olaf LU (2006) In Immunology 118(Jun 16). p.539-548
Abstract
Toll-like receptors (TLRs) recognize specific pathogen-associated molecular patterns (PAMPs), which subsequently trigger innate immunity. Recent data also suggest a role for TLRs in the direct activation of adaptive immune cells. In the present study, the expression and function of TLR1-TLR10 were characterized in purified human tonsillar B cells, focusing on differences among CD19(+) CD38(-) CD27(-) (naive B cells), CD19(+) IgD(-) CD27- [germinal centre (GC) B cells] and CD19(+) CD38(-)CD27(+) (memory B cells) cells. The study was also designed to compare the TLR expression in B cells obtained from infected and hyperplastic tonsils that served as controls. The results demonstrated a distinct repertoire of TLRs, in which TLR1, TLR2, TLR7,... (More)
Toll-like receptors (TLRs) recognize specific pathogen-associated molecular patterns (PAMPs), which subsequently trigger innate immunity. Recent data also suggest a role for TLRs in the direct activation of adaptive immune cells. In the present study, the expression and function of TLR1-TLR10 were characterized in purified human tonsillar B cells, focusing on differences among CD19(+) CD38(-) CD27(-) (naive B cells), CD19(+) IgD(-) CD27- [germinal centre (GC) B cells] and CD19(+) CD38(-)CD27(+) (memory B cells) cells. The study was also designed to compare the TLR expression in B cells obtained from infected and hyperplastic tonsils that served as controls. The results demonstrated a distinct repertoire of TLRs, in which TLR1, TLR2, TLR7, TLR9 and TLR10 predominated. No differences were found among naive, GC and memory B cells. Tonsillar infection did not substantially alter the TLR expression profile in ex vivo-isolated B-cell subsets. Purified CD19+ B cells stimulated with Pam(3)CSK(4), R-837 and CpG oligodeoxynucleotide (ODN) 2006, via TLR1/TLR2, TLR7 and TLR9, respectively, showed an induction of interleukin-6 secretion and an up-regulated expression of human leucocyte antigen (HLA)-DR. Collectively, the present study demonstrates that B cells exhibit constitutively high levels of specific TLRs, which are not developmentally regulated during the B-cell differentiation process. Ongoing microbial infections, such as chronic tonsillitis, do not appear to affect the TLR profile in B cells. Furthermore, the distinct expression of TLRs allows B cells to.respond directly to the cognate PAMPs. This further emphasizes the role of TLRs in directly activating adaptive immune cells. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tonsillar B cells, infection, PAMPs, Toll-like receptors
in
Immunology
volume
118
issue
Jun 16
pages
539 - 548
publisher
Wiley-Blackwell
external identifiers
  • wos:000239961600011
  • pmid:16780564
  • scopus:33746053199
ISSN
0019-2805
DOI
10.1111/j.1365-2567.2006.02392.x
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Clinical and Experimental Allergy Research (013243510), Reconstructive Surgery (013240300)
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d0476981-e2f1-4e7a-a362-472fd3ca13c2 (old id 158282)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16780564&dopt=Abstract
date added to LUP
2016-04-01 12:14:42
date last changed
2021-06-08 02:58:00
@article{d0476981-e2f1-4e7a-a362-472fd3ca13c2,
  abstract     = {Toll-like receptors (TLRs) recognize specific pathogen-associated molecular patterns (PAMPs), which subsequently trigger innate immunity. Recent data also suggest a role for TLRs in the direct activation of adaptive immune cells. In the present study, the expression and function of TLR1-TLR10 were characterized in purified human tonsillar B cells, focusing on differences among CD19(+) CD38(-) CD27(-) (naive B cells), CD19(+) IgD(-) CD27- [germinal centre (GC) B cells] and CD19(+) CD38(-)CD27(+) (memory B cells) cells. The study was also designed to compare the TLR expression in B cells obtained from infected and hyperplastic tonsils that served as controls. The results demonstrated a distinct repertoire of TLRs, in which TLR1, TLR2, TLR7, TLR9 and TLR10 predominated. No differences were found among naive, GC and memory B cells. Tonsillar infection did not substantially alter the TLR expression profile in ex vivo-isolated B-cell subsets. Purified CD19+ B cells stimulated with Pam(3)CSK(4), R-837 and CpG oligodeoxynucleotide (ODN) 2006, via TLR1/TLR2, TLR7 and TLR9, respectively, showed an induction of interleukin-6 secretion and an up-regulated expression of human leucocyte antigen (HLA)-DR. Collectively, the present study demonstrates that B cells exhibit constitutively high levels of specific TLRs, which are not developmentally regulated during the B-cell differentiation process. Ongoing microbial infections, such as chronic tonsillitis, do not appear to affect the TLR profile in B cells. Furthermore, the distinct expression of TLRs allows B cells to.respond directly to the cognate PAMPs. This further emphasizes the role of TLRs in directly activating adaptive immune cells.},
  author       = {Månsson, Anne and Adner, Mikael and Höckerfelt, Ulf and Cardell, Lars-Olaf},
  issn         = {0019-2805},
  language     = {eng},
  number       = {Jun 16},
  pages        = {539--548},
  publisher    = {Wiley-Blackwell},
  series       = {Immunology},
  title        = {A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by PamCSK, R-837 and CpG-2006 stimulation.},
  url          = {https://lup.lub.lu.se/search/files/2843086/625500.pdf},
  doi          = {10.1111/j.1365-2567.2006.02392.x},
  volume       = {118},
  year         = {2006},
}