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Light-Induced Anticancer Activity of [RuCl2(DMSO)4] Complexes

Brindell, Malgorzata LU ; Kulis, E; Elmroth, Sofi LU ; Urbanska, K and Stochel, G (2005) In Journal of Medicinal Chemistry 48(23). p.7298-7304
Abstract
The cytotoxicity and photocytotoxicity of trans-[RuCl2(DMSO)4] and cis-[RuCl2(DMSO)4] complexes was tested in two melanoma cell lines, human (SK-MEL 188) and mouse (S91). The trans isomer was found to be more effective for cell growth inhibition than its cis analogue both in the presence and in the absence of illumination. However, the antiproliferative activity of both isomers was significantly enhanced after irradiation with UVA light in comparison with their activity observed in the dark. The influence of light on the reaction of both ruthenium(II) isomers with the single-stranded hexanucleotide d(T2GGT2), chosen as a model system for DNA, was also studied using chromatography and mass spectrometry techniques. The photochemical reaction... (More)
The cytotoxicity and photocytotoxicity of trans-[RuCl2(DMSO)4] and cis-[RuCl2(DMSO)4] complexes was tested in two melanoma cell lines, human (SK-MEL 188) and mouse (S91). The trans isomer was found to be more effective for cell growth inhibition than its cis analogue both in the presence and in the absence of illumination. However, the antiproliferative activity of both isomers was significantly enhanced after irradiation with UVA light in comparison with their activity observed in the dark. The influence of light on the reaction of both ruthenium(II) isomers with the single-stranded hexanucleotide d(T2GGT2), chosen as a model system for DNA, was also studied using chromatography and mass spectrometry techniques. The photochemical reaction of the ruthenium(II) complexes with the oligonucleotide d(T2GGT2) resulted in the formation of Ru(G-N7)2 adducts, which was not observed in the same time scale in thermal reactions. The initial short irradiation of the inert cis isomer was found to facilitate the covalent adduct formation with d(T2GGT2) in the secondary thermal reactions and with a rate comparable to that found for the trans isomer, which is ca. 5-10 times more reactive in the dark. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Medicinal Chemistry
volume
48
issue
23
pages
7298 - 7304
publisher
American Chemical Society (ACS)
external identifiers
  • wos:000233399500026
  • pmid:16279789
  • scopus:28544448983
ISSN
1520-4804
DOI
10.1021/jm0502992
language
English
LU publication?
yes
id
5695d8de-06b9-4cc1-9879-7bb4d3975493 (old id 158589)
date added to LUP
2007-07-04 14:09:04
date last changed
2017-10-22 03:57:59
@article{5695d8de-06b9-4cc1-9879-7bb4d3975493,
  abstract     = {The cytotoxicity and photocytotoxicity of trans-[RuCl2(DMSO)4] and cis-[RuCl2(DMSO)4] complexes was tested in two melanoma cell lines, human (SK-MEL 188) and mouse (S91). The trans isomer was found to be more effective for cell growth inhibition than its cis analogue both in the presence and in the absence of illumination. However, the antiproliferative activity of both isomers was significantly enhanced after irradiation with UVA light in comparison with their activity observed in the dark. The influence of light on the reaction of both ruthenium(II) isomers with the single-stranded hexanucleotide d(T2GGT2), chosen as a model system for DNA, was also studied using chromatography and mass spectrometry techniques. The photochemical reaction of the ruthenium(II) complexes with the oligonucleotide d(T2GGT2) resulted in the formation of Ru(G-N7)2 adducts, which was not observed in the same time scale in thermal reactions. The initial short irradiation of the inert cis isomer was found to facilitate the covalent adduct formation with d(T2GGT2) in the secondary thermal reactions and with a rate comparable to that found for the trans isomer, which is ca. 5-10 times more reactive in the dark.},
  author       = {Brindell, Malgorzata and Kulis, E and Elmroth, Sofi and Urbanska, K and Stochel, G},
  issn         = {1520-4804},
  language     = {eng},
  number       = {23},
  pages        = {7298--7304},
  publisher    = {American Chemical Society (ACS)},
  series       = {Journal of Medicinal Chemistry},
  title        = {Light-Induced Anticancer Activity of [RuCl2(DMSO)4] Complexes},
  url          = {http://dx.doi.org/10.1021/jm0502992},
  volume       = {48},
  year         = {2005},
}