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Decreased Gene Expression of Fibrillin-1 in Stress Urinary Incontinence

Soderberg, Marie Westergren; Bystrom, Birgitta; Hammarstrom, Margareta; Malmström, Anders LU and Ekman-Ordeberg, Gunvor (2010) In Neurourology and Urodynamics 29(3). p.476-481
Abstract
Aims: Studies to show impairments in the pelvic floor extracellular matrix (ECM) associated with stress urinary incontinence (SUI) has earlier been performed, but the results are contradictory. Collagen I and III, the elastin associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Their gene signals and tissue localizations were investigated. Methods: Para-urethral punch biopsies were obtained from 24 women, 12 pre- and 12 postmenopausals, during surgery for SUI. As controls, biopsies were collected from 14 women, 8 pre- and 6 postmenopausals, undergoing surgery for other benign conditions. The mRNA... (More)
Aims: Studies to show impairments in the pelvic floor extracellular matrix (ECM) associated with stress urinary incontinence (SUI) has earlier been performed, but the results are contradictory. Collagen I and III, the elastin associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Their gene signals and tissue localizations were investigated. Methods: Para-urethral punch biopsies were obtained from 24 women, 12 pre- and 12 postmenopausals, during surgery for SUI. As controls, biopsies were collected from 14 women, 8 pre- and 6 postmenopausals, undergoing surgery for other benign conditions. The mRNA expression by real-time RTPCR and protein localization by immunohistochemistry were analyzed concerning collagen I and III, the small leucine rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin and the elastic fiber associated proteins fibulin-5 and fibrillin-1. Statistical comparisons controlled for age changes in gene expressions. Results: A significant decrease in mRNA expression of fibrillin-1 was discovered in all SUI women compared to all controls, P = 0.03. All molecules were down-regulated by age, but no other differences between SUI and controls reached significance. All proteins were adequately expressed by immunohistochemistry. A weaker staining for fibrillin-1 was seen in the pre-menopausal SUI group compared to the pre-menopausal controls. Conclusions: A decreased gene signal and weaker immunoreactivity for fibrillin-1, important for the elastic fiber assembly, was discovered in women with SUI. Loss of tissue elasticity could lead to increased urethra hypermobility and SUI. Neurourol. Urodynam. 29:476-481, 2010. (C) 2009 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proteoglycan, incontinence, extracellular matrix, collagen, elastin
in
Neurourology and Urodynamics
volume
29
issue
3
pages
476 - 481
publisher
John Wiley & Sons
external identifiers
  • wos:000276333400027
  • scopus:77953552222
ISSN
0733-2467
DOI
10.1002/nau.20735
language
English
LU publication?
yes
id
566b9dcc-2899-4c4d-8ce2-87ce55a8e755 (old id 1586948)
date added to LUP
2010-04-27 12:32:20
date last changed
2018-05-29 10:53:12
@article{566b9dcc-2899-4c4d-8ce2-87ce55a8e755,
  abstract     = {Aims: Studies to show impairments in the pelvic floor extracellular matrix (ECM) associated with stress urinary incontinence (SUI) has earlier been performed, but the results are contradictory. Collagen I and III, the elastin associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Their gene signals and tissue localizations were investigated. Methods: Para-urethral punch biopsies were obtained from 24 women, 12 pre- and 12 postmenopausals, during surgery for SUI. As controls, biopsies were collected from 14 women, 8 pre- and 6 postmenopausals, undergoing surgery for other benign conditions. The mRNA expression by real-time RTPCR and protein localization by immunohistochemistry were analyzed concerning collagen I and III, the small leucine rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin and the elastic fiber associated proteins fibulin-5 and fibrillin-1. Statistical comparisons controlled for age changes in gene expressions. Results: A significant decrease in mRNA expression of fibrillin-1 was discovered in all SUI women compared to all controls, P = 0.03. All molecules were down-regulated by age, but no other differences between SUI and controls reached significance. All proteins were adequately expressed by immunohistochemistry. A weaker staining for fibrillin-1 was seen in the pre-menopausal SUI group compared to the pre-menopausal controls. Conclusions: A decreased gene signal and weaker immunoreactivity for fibrillin-1, important for the elastic fiber assembly, was discovered in women with SUI. Loss of tissue elasticity could lead to increased urethra hypermobility and SUI. Neurourol. Urodynam. 29:476-481, 2010. (C) 2009 Wiley-Liss, Inc.},
  author       = {Soderberg, Marie Westergren and Bystrom, Birgitta and Hammarstrom, Margareta and Malmström, Anders and Ekman-Ordeberg, Gunvor},
  issn         = {0733-2467},
  keyword      = {proteoglycan,incontinence,extracellular matrix,collagen,elastin},
  language     = {eng},
  number       = {3},
  pages        = {476--481},
  publisher    = {John Wiley & Sons},
  series       = {Neurourology and Urodynamics},
  title        = {Decreased Gene Expression of Fibrillin-1 in Stress Urinary Incontinence},
  url          = {http://dx.doi.org/10.1002/nau.20735},
  volume       = {29},
  year         = {2010},
}