Decreased Gene Expression of Fibrillin-1 in Stress Urinary Incontinence
(2010) In Neurourology and Urodynamics 29(3). p.476-481- Abstract
- Aims: Studies to show impairments in the pelvic floor extracellular matrix (ECM) associated with stress urinary incontinence (SUI) has earlier been performed, but the results are contradictory. Collagen I and III, the elastin associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Their gene signals and tissue localizations were investigated. Methods: Para-urethral punch biopsies were obtained from 24 women, 12 pre- and 12 postmenopausals, during surgery for SUI. As controls, biopsies were collected from 14 women, 8 pre- and 6 postmenopausals, undergoing surgery for other benign conditions. The mRNA... (More)
- Aims: Studies to show impairments in the pelvic floor extracellular matrix (ECM) associated with stress urinary incontinence (SUI) has earlier been performed, but the results are contradictory. Collagen I and III, the elastin associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Their gene signals and tissue localizations were investigated. Methods: Para-urethral punch biopsies were obtained from 24 women, 12 pre- and 12 postmenopausals, during surgery for SUI. As controls, biopsies were collected from 14 women, 8 pre- and 6 postmenopausals, undergoing surgery for other benign conditions. The mRNA expression by real-time RTPCR and protein localization by immunohistochemistry were analyzed concerning collagen I and III, the small leucine rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin and the elastic fiber associated proteins fibulin-5 and fibrillin-1. Statistical comparisons controlled for age changes in gene expressions. Results: A significant decrease in mRNA expression of fibrillin-1 was discovered in all SUI women compared to all controls, P = 0.03. All molecules were down-regulated by age, but no other differences between SUI and controls reached significance. All proteins were adequately expressed by immunohistochemistry. A weaker staining for fibrillin-1 was seen in the pre-menopausal SUI group compared to the pre-menopausal controls. Conclusions: A decreased gene signal and weaker immunoreactivity for fibrillin-1, important for the elastic fiber assembly, was discovered in women with SUI. Loss of tissue elasticity could lead to increased urethra hypermobility and SUI. Neurourol. Urodynam. 29:476-481, 2010. (C) 2009 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1586948
- author
- Soderberg, Marie Westergren ; Bystrom, Birgitta ; Hammarstrom, Margareta ; Malmström, Anders LU and Ekman-Ordeberg, Gunvor
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- proteoglycan, incontinence, extracellular matrix, collagen, elastin
- in
- Neurourology and Urodynamics
- volume
- 29
- issue
- 3
- pages
- 476 - 481
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000276333400027
- scopus:77953552222
- ISSN
- 0733-2467
- DOI
- 10.1002/nau.20735
- language
- English
- LU publication?
- yes
- id
- 566b9dcc-2899-4c4d-8ce2-87ce55a8e755 (old id 1586948)
- date added to LUP
- 2016-04-01 10:02:38
- date last changed
- 2022-04-12 01:20:36
@article{566b9dcc-2899-4c4d-8ce2-87ce55a8e755, abstract = {{Aims: Studies to show impairments in the pelvic floor extracellular matrix (ECM) associated with stress urinary incontinence (SUI) has earlier been performed, but the results are contradictory. Collagen I and III, the elastin associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Their gene signals and tissue localizations were investigated. Methods: Para-urethral punch biopsies were obtained from 24 women, 12 pre- and 12 postmenopausals, during surgery for SUI. As controls, biopsies were collected from 14 women, 8 pre- and 6 postmenopausals, undergoing surgery for other benign conditions. The mRNA expression by real-time RTPCR and protein localization by immunohistochemistry were analyzed concerning collagen I and III, the small leucine rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin and the elastic fiber associated proteins fibulin-5 and fibrillin-1. Statistical comparisons controlled for age changes in gene expressions. Results: A significant decrease in mRNA expression of fibrillin-1 was discovered in all SUI women compared to all controls, P = 0.03. All molecules were down-regulated by age, but no other differences between SUI and controls reached significance. All proteins were adequately expressed by immunohistochemistry. A weaker staining for fibrillin-1 was seen in the pre-menopausal SUI group compared to the pre-menopausal controls. Conclusions: A decreased gene signal and weaker immunoreactivity for fibrillin-1, important for the elastic fiber assembly, was discovered in women with SUI. Loss of tissue elasticity could lead to increased urethra hypermobility and SUI. Neurourol. Urodynam. 29:476-481, 2010. (C) 2009 Wiley-Liss, Inc.}}, author = {{Soderberg, Marie Westergren and Bystrom, Birgitta and Hammarstrom, Margareta and Malmström, Anders and Ekman-Ordeberg, Gunvor}}, issn = {{0733-2467}}, keywords = {{proteoglycan; incontinence; extracellular matrix; collagen; elastin}}, language = {{eng}}, number = {{3}}, pages = {{476--481}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Neurourology and Urodynamics}}, title = {{Decreased Gene Expression of Fibrillin-1 in Stress Urinary Incontinence}}, url = {{http://dx.doi.org/10.1002/nau.20735}}, doi = {{10.1002/nau.20735}}, volume = {{29}}, year = {{2010}}, }